Light Microscopy: Function and Utility
ฝัง
- เผยแพร่เมื่อ 24 ม.ค. 2021
- Now that we know a bit about the history of microscopy, let's dig into the first form that existed, light microscopy. This is the form that is familiar to most of us, as the microscopes we all looked through in high school biology class were light microscopes. How do these work, what can we do with them, and how does one operate a light microscope? Let's get some tips on this technique now!
Script by Kia Mackey
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Your videos are essentially my new college biology course due to online learning and I am completely fine with it. You are an excellent teacher and are able to break down complex topics and communicate them extremely effectively. Thank you for all your time and effort that you put into these videos.
This "history of" approach is really working well, Professor Dave. I enjoyed this discussion of light microscopy almost as much as I enjoyed using my light microscope as a kid. Looking forward to your future discussions on different and more powerful microscopes!
I’m really excited for the electron microscope videos... I’ve been obsessed with trying to figure out how they work really work fundamentally. And all explanations I’ve heard to date have been seriously weak. Dave usually explains deeper concepts so I’m hoping he can address the underlying assumptions of physics/optics
Thank you for your explanations and easy to follow descriptions ❤
Thanks for your information, it is well done and it will help me in my microscopy test in the next 2 hours.
Great explanation professor!
Great explanation sir!❤
Perfect timing! I recently bought a microscope, you are the man Professor Dave!!
You still use it?
Nope.@@nyxdausen4552
Well explained 💯
☺️You are the best Professor in the world👨🏻🏫
thank u for such a tutorial
that was very good explain about light microscope
yaaaay thanks prof dave~
My biology teacher played this great work!
Amazing.🎉❤
Best teacher
What oil(s) does one use for oil immersion light microscopy?
Please make a video about the types of light microscopy
Thanks
I have to use microscope next month for practical and it's great video 📸
Impress your TA and do Kohler illumination.
Please make a video on Confocal Microscopy and Two photon microscopy
You have saved me !😂 Thanks ❤
Thank you so much
From India 🇮🇳 ♥️
Sir we want full explanation on techniques please upload it sir
Hey Professor Dave! I'm 46 and learned a lot from your channel. I enjoy the flat earth videos. I know, I know, I think they're idiots too. But my Grandfather was on the aircraft carrier Saratoga (CV-3) in World War II. I've been reading letters he wrote to my grandmother. I thought, how could The Pacific War happen in the time frame allotted if Earth was flat? Would love to hear you use that.
The Tiny Guitar and Fluit music is haunting me in my dreams... In a Good Way.
Bruh, if only I had this during my Bio practical Exams
I know there's and organic compound they already use but I want to know if you can think of something better
Love from India
hey Dave in a Rankine cycle or a closed loop turbine boiler system, what would be a good low boiling point fluid to use for boosting efficiency?...... and wont explode at 300-900'c as that would be bad preferably easy to get ahold of when I rebuild my prototype.
Dude i never thought about using oil to bridge the gap between both pieces of glass to avoid unnecessary diffraction... But it absolutely makes sense 🤔
This only works if the objective is rated for oil immersion. Not all lenses are the same. Even different oils have different indexes of refraction, and certain oils are designed to work with certain lenses. So you might need to check the specs to see which oil is right for you.
Also not all 100x lenses are oil immersion and using oil on a non oil lens can mess it up pretty bad. Not using oil on an oil immersion lens will not ruin the lens, but give you a terrible hazy image.
is the magnification of the light microscope equal to 1500 ??
I suppose I could look through catalogs, but I wanted to ask if light microscopes with television outputs that catch everything as though through the eyepiece were cheap and available? I'd figure that'd be a good classroom instrument.
Are you going to do more math/physics videos?
planning more physics now! hoping to do more math too
THAAAAAAAAAAAAAAAAANK U
Wow
I notice a distinct lack of Kohler illumination...
Now which one should I buy?
Buy a confocal. Leica makes good ones. You can get one pretty cheap...less than $100,000 if you spec it out right.
@@davyc412 over my budget. I'm trying to get the biggest bang for my buck around 250 dollars.
@@UncleSarkis Okay the confocal thing was a joke. In all seriousness though, put serious consideration into getting a used scope from a good brand like Leica, Nikon, Olympus, or Zeiss (they usually have the best optics), and if you just want a serious good scope, avoid bells and whistles like external screens or displays. All of those bells and whistles end up on two types of microscopes. (1) the super cheap stuff people end up buying for their kids from Walmart that never get used and work like garbage, and (2) the $10,000 + scopes that end up getting used in research labs.
the best optical bang for your buck is usually from the simplest of scopes where your money goes into quality optics. If the scope also has removable and interchangeable objectives, that's usually an okay sign that those objectives are pretty good. If the objectives have 160/ , 170/ ,or ∞/ engraved on the side that's also a decent sign that you might be dealing with quality optics, if they also have Nikon, Olympus, Zeiss or Leica engraved on the side, that's also a really good sign you are probably dealing with quality optics.
If you have a local university with a biology department, email them and see if they are throwing anything away. A few years ago, in the building I worked in, they were throwing away like 20-30 old Zeiss microscopes from the teaching labs that were super old (they looked like they were from the 60s) that were just taking up space in the teaching labs because nobody knew what to do with them. They ended up on a cart in the hallway with a "for free" sign. The thing is they don't look fancy at all, they look something you might find at a garage sale, its basically a massive black chunk of iron with an eyepiece, condenser, and objective turret, but the optics will blow anything out of the water that you could find new in store today for less than 250 bucks. I ended up picking through them and taking around 10 of them, one for myself and donated the rest to a school in my area.
Also, if you want to take pictures, having a scope with camera mount or at the very least a removable eyepiece is a must. you can get adaptors for stuff like Canon and Nikon DSLRS that will fit a microscope. They are a little finicky to figure out what you need, and to get set up correctly with the focusing distance, but they work really well once they are set up. We have a massive Zeiss trinocular scope in the lab that we use for basic phase contrast and its got just a plain old Canon Rebel T3i mounted on the top to take pictures with.
@@davyc412 I really appreciate the information. I'm going to try the University route first to see if I can get a timeless tool like you got.
Tip: use enough, that is bright, light or you will see a lot of artifacts. Learn how to properly make a specimen.
You da man. 😅
where is prism???
The star of the zombie movie "The Flatearth Zombie slayer"
Hi all
You know what else is hard
You’re pretty serious about this science stuff huh?
He wouldn't know so much about the science stuff otherwise
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uh yeah. He's a scientist...?
Huh after 2 years
lol
About bio electromagnetic rezonance, molecular frecvence resonance, 5G and living organism. Some scientific data?
Não existe sagrado?
1st like .
No one cares
@@Leolikat
And no one cares about your comment too .
You sound like adult sheldon.
the intro is dog water
Wow
Love from Pakistan