Did you know that TRIS will form precipitate with Ag+ in conventional pH meter electrode? One does not simply measure TRIS buffer in pH meter with Ag/AgCl electrode with single junction reference electrode (mostly used in conventional pH meter now days) because it will poisons the reference electrode. using calomel electrode or double junction reference electrode is the correct way it seems that your using single junction electrode (i can see it clearly) and therefore your actual pH in not 7.8.
I have a question, if I want to prepare a solution at a concentration of 100 mM pH: 7.8 from this solution(0.5M, pH:7.8), I have to re-measure your pH? Thanks for you video.
Thank you for the Video, it's really helpful. What we put instead of HCl to increase our pH Buffer solution?, for example if my buffer pH is 8,5 but I want it pH at 9.0
When i add the tris my solution becomes cloudy. I have to adjust the pH with hcl. Only after it will be clear again. I'm using 1 g/l of tris(THAM). Is this normal?
good video ,,thanks alot,,, but can you help me about the best buffer that can be used in my experiment when my drug is unstable at phosphate buffer,,,, means that when i prepared the phosphate buffer the uv absorbance was not stable that changed with time but i need the same absorbance for 14 days !!!! so what the solution ?? plz help me :((
Hamburgo pH paper can be faster since all you have to do is insert it into your solution and look for color change. pH meters are quantitative so you can determine the exact pH of your solution.
Did you know that TRIS will form precipitate with Ag+ in conventional pH meter electrode?
One does not simply measure TRIS buffer in pH meter with Ag/AgCl electrode with single junction reference electrode (mostly used in conventional pH meter now days) because it will poisons the reference electrode.
using calomel electrode or double junction reference electrode is the correct way
it seems that your using single junction electrode (i can see it clearly) and therefore your actual pH in not 7.8.
Great video, very helpful, but I think your N on the NaOH bottle is backwards.
no calculations mentioned, how do we know we need to add 15.14g of powder? where does it come from
If you know basic chem, it is just stoichiometry
The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer
Why we only use HCl and not any other acid for stabilizing ph please answer
how can we know to add 15.4g of tris buffer in sample water
can you make a video about how to prepare lysis buffer
I had no idea that you could add HCl to your buffer to alter the PH. my mind is blown.
Yes you r rite..it should not be done so
this is the correct way, what do you suggest@@sasidharankr2344
I have a question, if I want to prepare a solution at a concentration of 100 mM pH: 7.8 from this solution(0.5M, pH:7.8), I have to re-measure your pH? Thanks for you video.
Thank you for the Video, it's really helpful. What we put instead of HCl to increase our pH Buffer solution?, for example if my buffer pH is 8,5 but I want it pH at 9.0
NaOH pellets
Why we didnt use boric acid?
When i add the tris my solution becomes cloudy. I have to adjust the pH with hcl. Only after it will be clear again. I'm using 1 g/l of tris(THAM). Is this normal?
Thank you it helped me a lot !
Good video
good video ,,thanks alot,,, but can you help me about the best buffer that can be used in my experiment when my drug is unstable at phosphate buffer,,,, means that when i prepared the phosphate buffer the uv absorbance was not stable that changed with time but i need the same absorbance for 14 days !!!! so what the solution ?? plz help me :((
So amazing 💗💗💗
Very helpful thank you
how to prepare 10mM tris buffer needed for DNA extraction??
can you do a video on CTAB buffer?
can you please upload some video on kreb henseleit buffer?
Which powder u have used?
does it work with amylase?
What is the temperature and time for autoclaving?
Great 👍
Olá...muito legal.
Aren't ph meter bars aren't as accurate as ph paper?
You switched it. pH paper is not as accurate as pH meters
joeriv5462 That's weird?!! In our biotech lab they prefer us using ph paper and said it was accurate. I think its because its faster...
Hamburgo pH paper can be faster since all you have to do is insert it into your solution and look for color change. pH meters are quantitative so you can determine the exact pH of your solution.
joeriv5462 Sweet! Really appreciate it :D
No. They cannot be.
what!? I dont even know if I should autoclave or filter!
me neither
tnks to video
great
Mmmmnn😊
I'm spiderman
0:58 do NOT stick your scoopula directly into a reagent bottle!