Composition and prepration of 50x TAE Buffer stock (Tris acetate EDTA)

TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. In molecular biology it is used in agarose electrophoresis typically for the seperation of nucleic acids such as DNA and RNA. It is made up acetate buffer, usually at pH 8.3, and EDTA, which sequesters divalent cations. Here we have made 50x TAE buffer and mentioned a step wise protocol for prepration of 1 litre stock, for 2 litre you have to double the measurments and quantity of chemicls used. You can use it for electrophoresis with 10x and 1x measures according to your requirements. For more details or related quries, comment down below and make sure to subscribe ‪@ampliconsofbiotech‬

[@Parbat5234]

Good job,,,very informative vedio

[@ampliconsofbiotech]

Thank you

[@sukhadkural8698]

The method is from sambrook. I am keen to know about how you measured EDTA as 0.5M and maintained pH of 8.0

[@MeghaDavis]

Can u pls tell What is the concentration of edta

[@Parbat5234]

Keep it up

[@ampliconsofbiotech]

👍

[@vishnudhakad4452]

Good👌👌

[@ampliconsofbiotech]

Thank you

[@beinghuman4457]

What is X in 50X??
I mean what does X denote here...plzz tell i have my exams today

[@ampliconsofbiotech]

X basically denotes the concentration of the solution, like (50x) means 50 times!

[@beinghuman4457]

@@ampliconsofbiotech ..thankyou sir

[@Dr.sakshisharma5453]

Mam how to maintain the Ph of Tae buffer

[@ampliconsofbiotech]

Just how you do it for other solutions, by naoh and hcl