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- เผยแพร่เมื่อ 1 ต.ค. 2024
- Watch our new video on Masson's Trichrome Staining Protocol for Collagen Fibers. You will find the full staining procedure in detail, each stage and time for its implementation.
Masson's Trichrome Staining method is used for differential staining of collagen fibers and smooth muscular fibers in tissues such as skin, heart, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. As a result nuclei will be stained in black color, cytoplasm, keratin, muscles - red, collagen - blue, erythrocytes - yellow.
Masson's Trichrome Staining Protocol:
1. Weigerts hematoxylin - 10 min.
2. Dry slides without washing.
3. Alcoholic solution of picric acid - 4 min.
4. Wash the slides quickly in distilled water (3 - 4 sec).
5. Acid crimson fuchsin solution - 4 minutes.
6. Wash the slides in distilled water.
7. Phosphomolybdic acid - 10 min.
8. Dry slides without washing.
9. Massons anyline blue - 5 min.
10. Wash slides in distilled water.
11. Rehydrate, clear and coverslip.
- We recommend you to watch the video "Mason trichrome staining for connective tissue": • Masson trichrome stain...
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Can we use tap water instead of distilled water? Is there any critical issue by not using distilled water?
what about Bouin's solution?
That's the picric acid.
I want it's principles
Thank you for making this video! It was very informative. 😃
How can i get it? Where
how long it takes to do that and a waste of reagents
The staining protocol takes about 30 minutes. In this video we used a kit for 100 tests, also there are kits for bigger quantities of tests and they are usually used for automatic stainers.
Do we have to dewax first?
Please what stain can I use to demonstrate mitochondria?
For mitochondrial muscles patology u can use gomori tricrome, If I'm not wrong
How can I contact you
thank you