How to Prepare a Slide for a Cytology Evaluation

How to take any sample containing cells, whether an aspirate from an organ or mass, or a fluid aspirate, and make a slide that can be evaluated in house or sent to a pathologist/cytologist.
-
Intellectual Property Statement
The content of this course, video and procedures are protected under copyright and other intellectual property laws. This video and/or education information is ONLY for non-commercial private viewing and individual private use only.
Any distribution, sharing, copying, teaching, transmission, public performance, alteration, or reverse engineering outside private individual use of purchaser ( unless expressly authorized by SonoPath) is strictly prohibited and may result in criminal and or civil liability.

[@daomarlluveres9324]

Straight to the point and specific. Thanks.

[@irishguy200007]

I don't understand. I thought that for cytology the simple has to be of a fixed area and volume to count the number of cells in that test quantity?

[@michaelfishman7174]

I would use a small spray bottle with a fine mist to wash off the excess stain on the slides since the cells can be washed off easily if not careful.

[@DeannaLBx]

Thank you! Great instruction

[@borreliahunter140]

From those 3 containers, without replenishing the dip/diff quick media, how many slides can one make before having to discard & replace it?

[@alioznur6399]

great video, thank you

[@focusonyourgoal85]

Thanks

[@bobbiebreitenbach3548]

Thanks so much it has been a very long time since I have done a slide and need to do one on my pup

[@hammudyalabbuda355]

Thank you so much my dear 💐💐💐💐

[@ew1493]

The moment she put the lead pack on the needle i wanted to tear my hair out!! dont do that plz guys, you can pinch yourself, take it from someone who has been throught it!
But great video! really helpful, thank you

[@katcoe1079]

They is a special too that you are supposed to hold the lid with. Or failing that a peg works great

[@jatnarivas8741]

There is actually a safe way to do it: put the cap on the table, with the opening pointing to the hand that has the syringe, stop holding the cap (put the hand that had the cap behind your back if needed), then drive the needle into the cap only with the hand holding the syringe, as if you were trying to park it. Once the needle is inside the cap, you can use the other hand to finish "locking" it in. Since the needle is at no point pointing at your body this way, you won't stab yourself.

[@GabyAR7575]

thank you so much. can I ask what type of staining do you used?

[@milkywayranchsc]

Quik diff

[@kiroshki]

I know it is an old video but do anybody knows what kind of microscope is enough to do cytology reading for breeding purpouse? To compare/ side to side it with progesterone tests.

[@calarzt3453]

How to prepare hematoxylin stain ???step by step please

[@maureenhighkin3116]

Thank you for the information.

[@JA-eq5um]

recapping a needle is a no no

[@kiroshki]

Why? Its safer to dispose it that way

[@atmiles88]

kiroshki After a needle is used, it should be discarded into a sharps container-NOT a trash bag or any other trash can not dedicated for sharps. Attempting to recap a needle is a safety hazard because you put yourself at high risk for sticking yourself. It’s best to just not try.

[@atmiles88]

J A Yeah, I was seeing red when I saw that.

[@user-5er102erarea9]

Nah. Tha is debatable. Uncapped needles are a hazard too. Not every country has means to deal with that.

[@charitygeisler1356]

​@atmiles88 That's your opinion. Doesn't mean it's the right way to do it.

[@allenafricana8617]

somebody help me the name of those stain reagents were used..

[@cajunscorpion]

I use for the following : 95% alcohol, 100% alcohol, hematoxylin, vintage bluing, OG-6, EA-50, DI water, and xylene

[@arunkadian4780]

Good

[@TheSlahaltaweel]

than you v.much

[@s.rsharinisureshbabucreati3181]

slide dip what chemical pl send

[@user-fz3gn2ud1v]

Urdu