Mam i have watched almost many of your videos with sir while DNA protocol and CTAB preparations. All of these are well. Kindly upload further videos regarding gene mapping and gene constructions. Namsty to you and whole team who did this well.
Wow very nice dna bands how did you get it please share protocol in detail.. because my plant dna has lot of smearing and bands are not clear and lot of contamination also please help me
Does smears in the result show that we have a lot of contamination in our sample? We take 2 microliters of loading dye and 3 microliters of DNA .. so we have to change the setting of pipette to 5 microliters everytime we load them?
You can go for Rnase treatment or do some changes in protocol. Concentration of dye and DNA does not affect the quality, smears shows that the problem lies in protocol itself. Either protein or RNA must not have been removed completely.
The size of the DNA molecules can be estimated by comparing the position of the bands on the gel to a standard ladder of known sizes. The smaller DNA fragments will travel further down the gel, while larger fragments will move more slowly and remain closer to the top. The intensity of the bands can provide information about the amount of DNA present in the sample. More intense bands indicate a higher concentration of DNA.
Mam i have watched almost many of your videos with sir while DNA protocol and CTAB preparations. All of these are well. Kindly upload further videos regarding gene mapping and gene constructions. Namsty to you and whole team who did this well.
Very very good and informative video 🙏❤️ Love from Nepal
Very very good video with nicely performed experiment and explaination of each reagents and dyes role😊
Thank you so much
Really nice😊keep uploading such videos which will help us further👍
Also make video on how to interpret the results before and after PCR.
Sure
बहोत हार्ड .......👌👌
Kya bat
Excellent Bhava.
Thanks a lot
Very good...
Thanks
Nice video so thank you sir😊
Helpful video 👌👌
Stay connected
Wow very nice dna bands how did you get it please share protocol in detail.. because my plant dna has lot of smearing and bands are not clear and lot of contamination also please help me
Share your results on +919834128616 we'll help you with the protocol.
@@ampliconsofbiotech ok thank you
Why smeared DNA ladder occur?
If agarose is not dissolved properly, or ladder may be older/contaminated.
Thank you
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Does smears in the result show that we have a lot of contamination in our sample?
We take 2 microliters of loading dye and 3 microliters of DNA .. so we have to change the setting of pipette to 5 microliters everytime we load them?
You can go for Rnase treatment or do some changes in protocol. Concentration of dye and DNA does not affect the quality, smears shows that the problem lies in protocol itself. Either protein or RNA must not have been removed completely.
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Could you please help me with removal the smear and contamination..even after purification...
Thank you...
Which protocol you used for isolation and for which plant sample?
White light wale band hai kya
Yes
What do bands tells us ??
The size of the DNA molecules can be estimated by comparing the position of the bands on the gel to a standard ladder of known sizes. The smaller DNA fragments will travel further down the gel, while larger fragments will move more slowly and remain closer to the top. The intensity of the bands can provide information about the amount of DNA present in the sample. More intense bands indicate a higher concentration of DNA.
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