Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer? This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)
thank you, your classes are amazing and extremely well explained.
@Annelise, your presentation is clear, concise and very well presented. Thank you!
Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer?
This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)
Does anyone have any idea about how to calulate the Mean Fluorescence Intensity? I am using a Sysmex Ploidy Analyser.
Great lecture! I feel better prepared now for tomorrow's in-course assessment!
well explained , well done, thanks
Thank you, very clear explanation
Hello is there a class I can take up for flow cytometry
thank you, you explain clearly
This is beautiful.
Thank you very much 😊
Thank you Dear Annelise, super clear!, thanks again!