Protoplast culture method | Made easy | Bio science

[@raghavuluvagilu1962]

Now i am completely ready to perform protoplast culture in biotechnology lab exam, Thank you sir for your lecture with good explanation 🤗🤗

[@bioscience6853]

My pleasure

[@shiekhusman7721]

Thankyou sir

[@cheenabhogal9913]

You know what ur explain jst a day before exam ..i think you the best teacher then my college teacher ...so i say thnq soo much for letting us know soo much about a particular topic...❤❤❤i am dam sure no teacher specially my college teacher can tauhht me in this good way...keep growing and ese hi video banate rahiyee❤❤

[@gayatrip8540]

Your explanation is very well and easy to understand the topic thank you for your videos keep going all the best 🙂🙂🙂

[@abhijitmandal4181]

01:34 Protoplast culture is a method to obtain protoplasts by removing cell walls from plant, bacterial, or fungal cells.
03:08 Protoplast culture involves obtaining protoplasts from leaf explants through the process of sterilization.
04:42 Sterilization removes microorganisms from the leaf surface, making it pure.
06:16 Isolation of protoplasts from plant cells using cellulase enzyme
07:50 Protoplast fusion and cell division in plant tissue culture.
09:24 Callus differentiation leads to direct root and shoot formation.
10:58 Advantages of protoplastic culture: mixing of two genomes, used in crosses at interspecific, intergeneric, or intraspecific levels
12:31 Potato and tomato combine to form pomato, a new strain with desired properties.

[@jonathanmayers5355]

Thank you, this was an excellent video. It was very informative and thorough; I learned a lot and plan on watching more of your content.

[@bioscience6853]

Thanks

[@priyankagummula3720]

Sir pls explain all topics from botany 6th sem kakatiya University..ur explanation is very well .... Ur explaining briefly to us .....😍🙃🙂🙂🙂

[@Perryreads]

Thank you for teaching in a understanding language its topic was quite difficult to understand keep it up Man

[@bioscience6853]

My pleasure

[@shilpanerallaramulu4288]

Really very good explaination👌. I liked ur videos a lot and thanks for the information.

[@bioscience6853]

My pleasure

[@yogithayogi4311]

Just stunned for ur explanation ❤🎉

[@ggesdsdsdsd]

Please do this with pepper + tomato plant thank you.

[@IamLilibel]

Is there an alternative for N2NO3?? What is the purpose of it in your plate?

[@sseditzzz9831]

Excellent job sir....its so help full for me

[@bioscience6853]

Thank you and nice to hear this from you.

[@ShopperPlug]

This is really an excellent explanation.

[@bioscience6853]

Thank you

[@charithasomireddy2720]

Tqsm this is topicing is running in our class

[@bioscience6853]

☺☺

[@himaninegi9379]

So good
Explanation is veryyy nice❤️👍

[@bioscience6853]

Thank you

[@roshnibani8844]

Bro aap best ho✌️✌️

[@bioscience6853]

Thank you

[@Left-Lovers]

How can we isolate proplast single cells by our eye?

[@serwodevi1310]

The 2 and 3rd division occur in NM ? Plz tell

[@serwodevi1310]

Why put the diffrentiated callus put in test tube of NM .it is also in NM ?

[@RajdeepMohapatra]

very nicely explained

[@pendlithejaswini5305]

Sir can u give notes
Good explanation sir thanks for explaining in good way

[@bioscience6853]

drive.google.com/file/d/1-GrZtiyp8UWUQvcZlXtT0nyEI5EX-iuJ/view?usp=drivesdk
Click on this link and send a request for me. So that u can download. If it is opening directly then dont send any request

[@pendlithejaswini5305]

@@bioscience6853 it's not opening sir

[@aishwaryatonde5191]

Hello everyone, I create notes related to life science topics.
Here are the typed notes for Isolation of Protoplast
biologysafari.blogspot.com/2022/06/isolation-of-protoplast.html
FOR MORE NOTES PLEASE VISIT biologysafari.blogspot.com/?m=1

[@takini4918]

Thank you sir🙏

[@bioscience6853]

My pleasure

[@sumeshsudhakaran4823]

Sir one doubt...the protoplast fusing contains diploid nucleus right? Is it polyploid nucleus produced there?

[@markkrebs7474]

Yes it does but many plants are polyploid, so it really isn't a problem.
The correct term for this sort of polyploidy would be allopolyploidy.

[@Team_Prabhas89]

Tq

[@geetanjalisikdar2073]

Thank you sir!

[@bioscience6853]

My pleasure

[@serwodevi1310]

Plz tell sir tomarrow is my exam

[@afiyasana3099]

Thankyou sir🙏

[@bioscience6853]

My pleasure

[@VickyBhoy]

Thanks!😅

[@bioscience6853]

My pleasure

[@yallalarajeswari2455]

Technology protocol in sterilization wash running water leaf micro organic substances surface fusion callus stemouter cell bacterial tissue green colour temperature isolation nitrogen

[@chandinik8822]

I wanna take ur notes but ur what's up group is full,as it's not allowing🥺

[@aishwaryatonde5191]

Hello everyone, I create notes related to life science topics.
Here are the typed notes for Isolation of Protoplast
biologysafari.blogspot.com/2022/06/isolation-of-protoplast.html
FOR MORE NOTES PLEASE VISIT biologysafari.blogspot.com/?m=1

[@rajkumarlnj9278]

Bro plss start bsc biotechnology 1st year lectures plsss

[@bioscience6853]

Already uploaded half of the topics. Please checkout our channel

[@yaxir5]

👍

[@manikantaa2554]

Bro cover bsc agriculture.. Plant tissue culture syllabus topic s

[@bioscience6853]

Ok bro

[@cginfotach]

Hindi me bhi boliye sir Hindi medium walo ko thoda dikht jata

[@RajdeepMohapatra]

bro please cover lectures of bsc agriculture

[@lilibeldelapuente2163]

Can you do a video on fungal somatic fusion with chitinase? Do you use the fruiting body?

[@aishwaryaudhayakumar7339]

Bro upload vedio on terminator seed concept bro

[@bioscience6853]

Sure

[@vivanbhati1977]

Where is tge notes?

[@aishwaryatonde5191]

Hello everyone, I create notes related to life science topics.
Here are the typed notes for Isolation of Protoplast
biologysafari.blogspot.com/2022/06/isolation-of-protoplast.html
FOR MORE NOTES PLEASE VISIT biologysafari.blogspot.com/?m=1

[@curiousneko]

01:50

[@yallalarajeswari2455]

Sir

[@DeepakSaini-jb6cq]

😒😒🤔🤔