Protoplast Fusion

This lecture explains about the protoplast fusion technique also known as somatic cell fusion which allows us to produce plant cell hybrids with increased drought tolerance and immunity.
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Thank you for watching

[@harishobla3492]

Nicely explained.
One suggestion.
could have added some points regarding fusionogen like poly ethylene glycol as questions are asked regarding fusionogens

[@ashfaqpeerzade166]

Pata hai poster presentation ke liye ye topic choose kiya tha aur TH-cam pe pehla video mere cutie pie ne banaya hai tqsm❤️

[@shomusbiologyofficial]

You're welcome

[@chiraggumbira]

thank you Mr.Shomu! your videos are a true tool in revising and understanding concepts!

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@muruliyadavn9742]

Sir thank you I got 65 marks in csir NET..next time I definitely clear the exam..because of u only tqsm😍

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@bmbijayanee8399]

Your video always best,but in this video if you explain the electro fusion method and chemical fusion method in detail then it should be more better.

[@thetrackx]

Nice Video!
Can you explain a bit more about the electro-fusion-process please? I want to experiment a bit with this technology.
Cheers.

[@nemo9396]

What tools and chemicals do you use for the fusion process? Also how do you select the successful hybrid, since I would assume you would do this with a whole mixture of cells, and you can have all sorts of fusion combinations.

[@khushirai1390]

@nemo9396
The chemical which is used to fuse the protoplast is actually a reagent called polyethylene gycol.
Pulsed electric field is also used to fuse them

[@swapnilnavghare1414]

thanks sir for better understanding than our teachers

[@shomusbiologyofficial]

You're welcome

[@최찬욱-k5k]

명쾌한 설명 고마워요 항상 유튜브 잘보고 있습니다 ^-^

[@MrGbhattacharyya]

Is it drag a cell wall out or removing the cell wall using the appropriate enzymes to be precise digesting the cell wall materials [initially it wasn't said]... Apart from using electricity there are chemical mediators , what are they called, (the fusogen isn't it, like PEG, sodium nitrate) such chemicals even fuse two different nuclei... Dr G Bhattacharyya

[@mfbvlog]

Your lecture always evergreen ❤️

[@shomusbiologyofficial]

Thank you

[@rameshthapa6530]

@@shomusbiologyofficial sir what kind of enzyme we can use for oyster mushroom(pleurotus ostreatus) to get high yeild protoplast...

[@basketballgawd18]

"right? obviously." :D thanks sir!!

[@officialsmaazzainiyyah]

is this process is part of conventional biotechnology?

[@anweshamohapatra9777]

Sir...
Please reply what is the difference between protoplast fusion and heterokaryon??

[@rudraseth5419]

really bhaiya aap jab kise chiz ko samhajte ho ...science ekdum. movie lgti h

[@TomsonGaming]

I study plant biotechnology this is very useful.

[@sangavibiochem1755]

Very use video thanks.... upload full video on somatic hybridisation in plant....

[@drujjawalrathore]

Sir,
How is the ploidy restored ...? Fusion of two diploid protoplasts will result in a tetraploid cell...

[@pokegamerz5405]

My exact question

[@Aaron-fe6le]

One method is obtaining the sexual organs from the tetraploid plant to develop a haploid culture through micropropagation.

[@AnkeetKumar]

What's the benefit of original ploidy.

[@mdarwin354]

Yes then how

[@swetasingh1331]

your explanation is the best

[@shomusbiologyofficial]

Thank you. Glad you liked it

[@babulaldhaka2989]

Sir u want to more videos on molecular biology for JRF and other compitativ exam....so so thanks for it...

[@bivinkrishna588]

Explained it very well......loved it 😍

[@krupalipatil6693]

Thank you

[@shomusbiologyofficial]

You're welcome

[@nikeetamishra2712]

Great teacher

[@shomusbiologyofficial]

Thank you

[@mohammadramjan2185]

Very good concept clearing deliver sir..

[@shomusbiologyofficial]

You're welcome

[@iraqi3612]

thanks so much doctor

[@shomusbiologyofficial]

You're welcome

[@sarmisthachanda3079]

Thank you sir🙏

[@shomusbiologyofficial]

You're welcome

[@ju7062]

You are AWESOME !!!!! Thank you !!!!! Microbiology student

[@aumvyas1214]

Perfect

[@shomusbiologyofficial]

Thank you

[@awakenedsouls3206]

How do the two nucleus know that they have to fuse ? Why don't they go mix with the cytoplasm of another cell?

[@sankarmati8638]

Ty so much sir plz explain about probes

[@shomusbiologyofficial]

You're welcome

[@sankarmati8638]

Plz sir explain about probe it's looks difficult to me

[@madeline_parks]

How do you prevent cells in the culture from fusing with their own species? Wouldn't you theoretically have two pure fused cell strains and one hybrid fused strain in the culture? Do you use a selective medium?

[@imseepingwithoneeye1217]

Thank you dada.
Apni onk help korlen amr

[@pihubabu5868]

Nice explanation sir

[@shomusbiologyofficial]

You're welcome

[@alaamohamed3211]

Thanks sir

[@krupalukikrupa2349]

Thank you so very very much sir🙏🙏🙏🙏

[@shomusbiologyofficial]

You're welcome

[@lipibhuyan5158]

Thanks a lot

[@shomusbiologyofficial]

You're welcome

[@okpararita2961]

Thank you Sir

[@shomusbiologyofficial]

You're welcome

[@helanmichael180]

Sir.. Plz make a playlist of mass transfer and o2, heat transfer coefficient

[@niks8924]

Sir what kinds of chemicals are required to perform this technique...?

[@hatredknight9964]

Bengalis are the best teachers

[@madeline_parks]

Do you use antibiotics to prevent bacterial contamination?

[@shrutimagotra4761]

Sir plz ..make a video on cybrids .

[@namratakhot2745]

which chemicals or reagents can be use to fuse the cells ...?

[@bhavanipattar446]

excellent teacher

[@nehadwivedi4959]

So nice sir

[@karanhatkar6560]

sir,in bitechnology we see much complexity about chromosomal and DNA mixing. Is it simple in somatic hybridisation????

[@akshitanayak9453]

Eukaryotic cells does not have cell wall , does it called protoplast?

[@arulkumar2958]

Nice

[@shomusbiologyofficial]

Thank you

[@bt-029satyakipahari5]

Sir ......in some place of the video your voice is inaudible.........

[@meghas3935]

cn u explain hw protoplast isolate and its culture

[@vibhadubey9079]

Thnk u so much sir.......

[@shomusbiologyofficial]

+Vibha Dubey you're welcome

[@thinkiestar]

Wow i hope i can do my exam well tomorrow

[@shomusbiologyofficial]

You're welcome

[@ritesh7713]

Thanks sir for this video

[@alizehkhan3809]

Thanku so much

[@mandipharan]

U r like my big of my colleg life # ager ap na hote to b pharm crack karna muskil tha bhai

[@babulaldhaka2989]

Sir so so thanks

[@dr.sarithapandit5224]

superb...

[@meenasuman1212]

Thank u sir

[@subodhkumarsharma8977]

Its too much difficult to understand 😢

[@saniyamulani9040]

Can we do protoplast fusion in bacteria?

[@shomusbiologyofficial]

No

[@tanu6048]

Sir plz mention limitations also

[@subodhkumarsharma8977]

Your voice and video playing not matching

[@remsangalianhna6086]

will they resemble the cell wall again

[@nitashasharma4510]

What's about the chromosome no. inside the cell????

[@kazisamiulislam9785]

if 2 protoplasts fused the chromosome number will be 4n?????

[@rockoutntopout]

Also following

[@JMSouchak]

I believe so. But plants can survive being more that 2n much better than animals and assuming that you are fusing two of the same species you will mostly have double copies of the same data... The exception being whatever unique trait or resistance you are aiming to copy.

[@LKVPAWAR_ycmou]

can there be the 4n in the up coming or dividing cell

[@rupertrankin489]

@@JMSouchak assuming hybridisation between sub-specific taxa as you say: a trial field, a keen undergraduate assistant and use of assisted sexual hybridisation still offers far superior odds on the progeny succeeding to maturity. Sexual method, one batch 100,000 successful seedlings, ready to observe in the field within a year and give immediate marketable clones. Time to acclimatisation and desired-trait testing is 1/3 that of expensive labrat plants. Using PF to generate 100,000 novel combinations? you'd spend the entire department's budget and several years on the project. Omg. PF is for getting a cross between impossibles. Who p-fuses clones of potato to transfer disease resistance?

[@patelvitixa656]

Callus cell meaning??

[@karanhatkar6560]

sir, potato+tomato=pomato. Is pomato is better than it's two parent????

[@hdkdjd2949]

karan hatkar Ya of course
Pomato has quality of both
Juicy like tomato & full of starch like potato

[@thilagavalli392]

Sir easily understand this topic super sir

[@badammanideep9924]

It is a weedic plant which doesnot have juicy fruit or neither starch filled fruits

[@nihala1234]

It's not much better as the parents, it's only an experiment,

[@samiaislam5513]

Super

[@ashishrout1947]

That's the way..maahii ve...🤣🤣

[@shomusbiologyofficial]

Thank you

[@ashishrout1947]

@@shomusbiologyofficial 👍✨

[@satyendramanhar862]

Hindi me hai kya sir apka video ya note hoga to please dene ki kripa kare

[@shomusbiologyofficial]

No. Note available only in english

[@sindhizone5834]

Voice was lagging first time got dis appointed

[@sarveshmaurya8912]

sir
hindi bhasha ka use kare

[@arvindkumarrajsus]

Please speak in hindi

[@rupertrankin489]

I quite agree, your english and your incoherent progress through the material was so hard to follow, maybe in Hindi you'd be in your element. Anyway Protoplast Fusion is still illegal in many English-speaking countries because it produces highly unreliable outcomes. Science is not safely held in the hands of big business without regulation by those outside of its circle of profitability. Perhaps you should speak in Hindi to protect what you are doing from outside scrutiny. It appears that the law in India is not much involved in keeping people safe, despite Bhopal etc. It also appears that due to your homey style, your appreciative audience hasn't realised that you are science-grooming a generation to accept an unacceptable practice - it will soon be 'oh no that's fine, we learned it in school' but it will have diverse applications some not regulated at all. People, look up and see past money for a few seconds to decide what world you want for your grandchildren! Ok that's it, back to your brilliant careers.

[@robinupadhyay5724]

Jayada angraji mat kate

[@shomusbiologyofficial]

Kuch nehi aata tujhe

[@G486-w6z]

Thank you sir..,

[@shomusbiologyofficial]

You're welcome

[@anjalinegi8543]

Thanxx sir