🔬 134 - How to GROW CELLS for microscopy at home | Amateur Science
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- เผยแพร่เมื่อ 20 มี.ค. 2020
- In this amateur microscopy video I will show you how you can make an enrichment culture for microscopy. This way you have a good source of paramecia and other ciliates for microscopic observation.
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I could watch these paramecium all day.
Please add the magnification used on screen, would be good to know, thanks
Yesssss
I seen on the one comment that he replied to was 40x - 200x
What a great channel. I love your video's and great enthusiasm. It's really helping me as a beginner and firing up my ever growing interest. Thank you for these video's, I love them. I'm especially impressed with how you advise on safety, economic value, tech..........everything. Great job.
About 6 months ago i took a patch of moss from a nearby forest, put it in a glass jar and added a bit of water. Now, the original rotifers, tardigrades, nematodes etc. have all died and have been replaced by other micro organisms. The moss is still growing. I took a few drops of the water on the bottom of the jar and put that into another jar, filled it with regular tapwater and added a few slices of apple. Now, about 3 weeks later a lot of Colpodea and smaller organisms have been growing in the 2nd jar. Really fun to keep myself busy. Thank you for making these videos. Stay safe.
That’s so cool
Love you’re videos man . Keep up the work 👍
After watching a good handful of your videos, i have to subscribe! You're so informative and I love your video content.
Love the instructional videos. Good narration cam.
+Nathaniel Voth...Microscopy and astronomy are my favorite hobbies...I have a microscope, and a telescope!...Hobbies like collecting stamps and coins are dull, and boring!
Thank u soo much for teaching this method;this method will be useful alot
Another great video. I believe both cultures are suggested in my copy of Culture Methods for Invertebrate Animals by Lutz, Welch, Galtsoff, & Needham. A wealth of info there, but you make it most accessible.
Like easy food source what all can make in home I can say is the medium for bacterial petri dish. Just make one infuzion of little sugar,worm water,salt,vegeta or little soup cube and put in your water sample. For that dont use Agar or gelatine . Most of this kind of food source are used to big jars of pound water collections but works in small ones too. Potatos bits or bannanasare good too. Great videos and put more info Oliver. Dont stay in safe mode whit our microworld around us. ;)
Thank you once again.👍👍😀
Cool! Thank you.
Very helpful video, also new facecam is really good
I am using a green screen in the backgound. This new system makes narration a bit more interesting.
Hay infusion is also good for culturing microbes!
I loved this video,I have a microscope and I’m now a new sub thanks.
Well i have read somewhere about "Hay infusion" which is also a type of culturing method...can you please make a video on it....it would be great....thank you very much Oliver.....
Is there a wall chart or something that shows all the different micro organisms to help identify what we see in the microscope? If so, can you send a link to that?
I did it with boiled milk. But first took sample with paramecium on clean glass and using needle split it into droplets. Then selected ones only with paramecium and moved to jar. After week or so I had hundrets of them.
Now I can have friends :>
Haha nice one!
But what if they die on the slide :)
@@harsha6937 D:
@@harsha6937 no NO NO NO I’m screamING
Thanks! Now i have a pot full of them
Hi, I live in an area close to a brackish body of water. Do You know some microbes live in brackish?
Do ciliates live in dirt? I've collected some dirt and mixed jt with water, but once I put it under the microscope I didn't see any ciliates. I did see a lot of rotifers and nematodes
Thanks for great videos you make. What microscope magnification and camera did you use for this video?
I think it's ethier between 20x or 40x(objectives) 10x(eyepiece)
The camera is a DSLR camera from Nikon
The microscope is an Olympus CH40
I think the smaller ciliates in the video are called grenade worms
Hi Oliver
What are your recommendations on the safety measures regarding this kind of culturing method? I've heard hay infusions aren't completely safe given that the bacteria cultured are unknown. Thanks in advance
The safety measures are simple, keep your culture away from food areas and do not eat, smoke or rub your face when handling it, wash your hands well after handling the culture. After you finish using your slides you can put them on a container with a solution of bleach for a few minutes before cleaning the slides same for the tools you used to handle the sample, a glass dropper works well, if you use tweezers be aware they can corrode on the bleach so just leave them there for a short amount of time and that's mostly it. You can dispose the culture on the drain once you want to get rid of it, wash the container with bleach.
Edit. I should add, avoid as much as possible to handle the sample with sharp tools, one of the biggest reasons for accidental infections in labs are punctures with needles or blades used to handle the microorganisms. The bleach solution to disinfect your material should be at least 5% bleach (1 part bleach 19 parts water).
Generally infusions are indeed risky, if too concentrated. Do not overfeed, in this case the ciliates will eat them and keep bacterial count low.
I have a jar with water and it has been sitting in the balcony for nearly a week. I don't have wheat seeds. Can sesame seeds be used too?
I never tried it. Separate the water into different samples and then add sesame to one, rice to the other, milk to the third etc.
StarGazer45 if you want a really nice,
Booming population of paramecium follow this: get a sample of pond water (12-16oz) with a bit of algae (pluck it yourself, 4-6 tbsps is plenty). Then put in a couple small twigs, dead leafs and a decent amount of lettuce ripped (1-1.5 leafs). Leave the jar open and let sit for 3-5 days and boom. Instant thriving population (it will smell horrible, that’s how you know it’s the good stuff)
@@Microbehunter Oh, and I've seen videos that say it's a good idea to put some dirt. Should I try it with a fourth jar?
@@lliillIIIIIIIIiIiiilllllll is it health hazard?
because you said it was smelly
Im about to do experiment with water and seed leave water within 5 days and observe it.
Is it possible to grow a stentor?
🌱
How do you create your spectacular backgrounds with your microscope?
I film myself in front of a "green screen" and I also make a video with the microscope (separately). The video editing program will then remove the green background and replace it with the video from the microscope. Search for "green screen" for instructions on how to do this.
@@Microbehunter ....Thank You!
Hi how are you i wanted to know how to find microbes and i dont have pond water so where to find them
Watch this here: th-cam.com/video/h0PwPRhsjTk/w-d-xo.html
@@Microbehunter Thank you also i am sorry
can I use bread instead of wheat grain?
Yes, but the bread becomes very soggy and starts to fall apart, and therefore it is difficult to take out again to get to the microogranisms.
Which magnification level??
Between 40x and 200x. Anything more is already difficult because the field of view is so small and the cells quickly move out of the field of view and are difficult to chase.
@@Microbehunter so much thanks for your kind reply
Can u make video on agar
2:29 that's a flagellate not a ciliate.
That was another great video...but listen Oliver, you must stay safe from any disease(talking about Corona virus only) as you are very important person for us.....please for God's sake take care of yourself...please...Let Lord Jesus protect you from this disease.....
Jesus? I find that particularly offensive. It's the world's scientists working hard to protect us.
Thank you. I am fine and so far healthy!
can I use powdered milk?
I see no reason why not but I never tried it. Just a small amount (tip of a knife). Overfeeding is easy becasue it is concentrated.
What will happen if I over feed them 😲😲
@@annfambeck2132 Too much food will cause the number of bacteria to go up too quickly. This reduces the oxygen content and then the paramecia can also not grow.
Oh my god😢😢
I kinda love green screen effect 😂😂😂
I was struggling to grow paramecium when I realized I was using almond milk instead of regular milk
Try looking at bath water
I don't think so , cause bath water should have soap and microbes does not like soap
@@annfambeck2132 What about Belle Delphine's bath water while its without soap
🤔🤔
Why are u uploading videos soo late
the smaller ones are flagellates
I know how to grow any cells without help but i need a help how to grow bacteria cells...
Agar
Let's say I live in the Desert and we have water dishes that sit outside and grow algae in them would this be a sufficient water source for potential micro organisms if I can't travel far? The water dishes sit under a tree we grow and sometimes collect leaves in the summer which adds more plant matter in a way for micro organisms. Would it be possible if I scrapped up some algae from that old water dish during a warm day would I be able to find any micro organisms? Water can't really pond up around here in the desert :(
The easiest thing is to try it out! If algae are growing, then other microorganisms are most likely also growing.
@@Microbehunter Neat! I’ll definitely give it go when things are just right, thank you for your response! I haven’t acquired a microscope yet so I’ve been watching loads of videos on microscopy I’ll have my own soon, I’m excited for the adventure and really enjoy learning about microbes!
@@Microbehunter I just got a Microscope today and scrapped up some algae from the water dish! I gasped when I saw a Rotifer for the first time under the microscope!
*Do not ask me how to grow rotifiers.*
How do you grow rotifers? (sorry :P)
@@danielle7760 Uh, it is complicated.
@ I see, I see... Tell me more~ (òwó)
@@danielle7760 I can tell you ways how to grow Lepadella ovalis at home..
1. Pour fresh water into a cut plastic bottle cup
2. Put in leaves, peas, leek and let stay for 2 months (pour unchlorinated water to prevent drought every month or every 3 weeks cuz evaporation) and put the cut plastic bottle with fresh water and leaves between windows (historic palett windows are recommended, we have historic palett widnows at home.)
3. Put in after 2 months some types of moss and feed the microbes with leek, peas (might cause water greenish because algae grows and nutrients grow enough to feed Lepadella.
4. Repeat the same way in 2nd step every 3 weeks, to prevent evaporation.
5. After around 5 months, and repeating the same way in 2nd way and 3rd way you should observing sample of water. And Lepadella would be your best microfriend, you can move with movement of microscope.
6. Still feed the rotifiers with peas, leek and basil and repeat the same way in 2nd way and 3rd way, to prevent evaporation. But the water would be more greenish.
7. The procedure must not be disturbed
@ D:
We need to see Corona virus
th-cam.com/video/h7ThRlqXkts/w-d-xo.html
HOW TO START A NEW PANDEMIC .NICE
What XD
NO- DON'T- NOT YETTTTT *proceeds to dramatically scream*
And create a new superbacteria??? No thx
What XD
nothing new, always the same