Hello! I am in Mexico, the video was very helpful, what applications are useful with Oxford Nanopore specifically in the human diagnostic setting or in any other area? Thanks! Thumbed up the video :D
Hi Alejandro, I'd recommend signing up for the Nanopore Community site, they have lots of really interesting information about how ONT technology can be applied: nanoporetech.com/community.
Hi Juliana, For each sample, you need to input 400 ng DNA, which is ~40 ng/ul once you account for the barcode you added. You then pool these and take 10 ul from that, which will still be 40 ng/ul. More than getting an exact concentration though, it's more important to get equal amounts of DNA across all the samples. This is why we've found that normalising it to fmol rather than ng and kb works best (as you are unlikely to get all of your extractions exactly the same size and this means you will have more or less DNA when you input).
@@pandora-id-netconsortium1846 This is very useful! thanks! another question: The protocol says that this kit is for gDNA with >30kb fragments. My gDNA is between 10 to 20kb. Can I still using it?
@@julianasoto5152 Hello thank you for the video. I have 3000kb fragment the HIV pol region can I still use it? is there a special primer adaptors I need to add on my nested step?
really helpful. THANK YOU VERY MUCH
You're welcome!
Quite helpful and informative. Thank you
Many thaks for sharing this! How can i get the prtocol from A-Z? Thnanks
Hello!
I am in Mexico, the video was very helpful, what applications are useful with Oxford Nanopore specifically in the human diagnostic setting or in any other area?
Thanks! Thumbed up the video :D
Hi Alejandro, I'd recommend signing up for the Nanopore Community site, they have lots of really interesting information about how ONT technology can be applied: nanoporetech.com/community.
What is the ideal concentration of the pooled library?
Hi Juliana,
For each sample, you need to input 400 ng DNA, which is ~40 ng/ul once you account for the barcode you added. You then pool these and take 10 ul from that, which will still be 40 ng/ul.
More than getting an exact concentration though, it's more important to get equal amounts of DNA across all the samples. This is why we've found that normalising it to fmol rather than ng and kb works best (as you are unlikely to get all of your extractions exactly the same size and this means you will have more or less DNA when you input).
@@pandora-id-netconsortium1846 This is very useful! thanks! another question: The protocol says that this kit is for gDNA with >30kb fragments. My gDNA is between 10 to 20kb. Can I still using it?
@@julianasoto5152 Hello thank you for the video. I have 3000kb fragment the HIV pol region can I still use it? is there a special primer adaptors I need to add on my nested step?