A Guide to Next Generation Sequencing Basics and Terminologies | Bioinformatics 101

In this video, I delve into the intricacies of a standard workflow for next-generation sequencing (NGS). We'll explore essential terminologies and concepts, including sequencing library preparation, multiplexing, sample pooling, as well as the roles of adaptors and barcodes. Furthermore, I provide an overview of different generations of sequencing technologies, spanning from the traditional Sanger sequencing to the advancements seen in Illumina, Ion Torrent, PacBio, and Oxford Nanopore sequencing technologies. I hope you find this video helpful! Leave your thoughts in the comment section below!
▸ Bridge Amplification:
1. • Overview of Illumina S...
2. • Next Generation Sequen...
▸ Sequencing by Synthesis (Illumina):
1. • Intro to Sequencing by...
2. • Learn about Illumina's...
▸ What is sequencing depth/depth of coverage?
• What is sequencing dep...
▸ Cost to coverage calculator:
1. geneglobe.qiagen.com/us/tools...
2. support.illumina.com/download...
▸ What is a fastq file?
• Understanding Bioinfor...
Chapters:
0:00 Intro
0:51 What is Next Generation Sequencing?
1:29 Evolution of sequencing technologies
4:10 A typical NGS workflow
5:01 What is library preparation?
5:47 What is a Flow cell?
7:28 What is multiplexing?
8:59 Index vs barcode
9:17 How many samples to multiplex?
10:39 What is a sequencing library?
11:01 Sequencing run
11:35 Output from sequencing run - fastq
You can show your support and encouragement by buying me a coffee:
www.buymeacoffee.com/bioinfor...
To get in touch:
Website: bioinformagician.org/
Github: github.com/kpatel427
Email: [e-mail]
#bioinformagician #bioinformatics #illumina #bridgeamplification #sequencingbysynthesis #multiplex #alleles #10x #oxfordnanopore #pacbio #affymetrix #barcode #setseed #reproducibility #pseudorandom #singleR #singlecell #annotationdbi #reversestranded #directstranded #strandedness #survival #survminer #survivalanalysis #kaplanmeier #tcga #gdcportal #tcgaportal #nci #cran #bioconductor #funcotator #variantcalling #variants #gatk #vcf #gvcf #haplotype #alleles #geneticvariants #mutations #gff3 #gff #gtf #sam #bam #phred #fasta #fastq #singlecell #10X #ensembl #biomart #annotationdbi #annotables #affymetrix #microarray #affy #ncbi #genomics #beginners #tutorial #howto #omics #research #biology #GEO #rnaseq #ngs

[@gauravnjoshi]

Concise, precise and to the point. Thank you 🙏

[@traveller_of_lyf]

So informative!! And easily explained!! Perfect video for an in-depth overview of NGS. 👍

[@sebastianrodriguez1021]

Thanks for this! It was a very nice explanation. Kind regards from Ecuador.

[@sanjanaghosh7]

Can you make a video elaborating about the data analysis? It would be of great help. Thank you.

[@chatomics]

A nice overview! Thanks!

[@fosterjane6596]

I found it informative. Thank you. !!

[@dr.hanyghazal3003]

Please, increase your frequency of uploading these great videos.

[@KasraArbabiZaboli]

Many Thanks for your great comprehensive videos. they are really helpful. Could you please make another video for WES analysis pipeline on linux? or at least mention the major differences of WGS and WES analysis steps? looking forward your useful help. thanks

[@KasraArbabiZaboli]

thanks a lot for your comprehensive videos. could you please make another video for Whole Exome Sequencing analysis Pipeline or at least describe the differences between WGS and WES analysis piplines? many thanks

[@oumarsako8201]

Thank you a lot.

[@Ankityadav-gq8os]

Very informative video i have a one Question for you is there any difference between DNA fastq file and RNA fastq file? How can we define that without doing any analysis on that

[@mahachaccour6115]

thank you for your videos. can you please make a video about how to use scGPT.

[@Wuhan.Huazhong]

can you please talk about basics commands and pipeline for ATAC-seq, Cut and tag-seq

[@amritabhattacharjee4596]

can you plz upload the papers/provide the link of the papers that you cited in the video?

[@Bioinformagician]

Sure, here you go:
1. www.ncbi.nlm.nih.gov/pmc/articles/PMC4633438/
2. www.pacb.com/blog/the-evolution-of-dna-sequencing-tools/
3. www.thetech.org/ask-a-geneticist/articles/2019/sanger-vs-next-gen-sequencing/
4. thesequencingcenter.com/cost-savings-with-multiplex-samples/