You are welcome. The whole point of defining threshold is to select the specific area of interest for analyzing. The image may contain debris or noise that needs to be excluded during thresholding. Performing a correct thresholding is necessary to maintain accuracy of quantification. There is a debate amongst the scientific community, whether to keep the thresholding value same or different across all the images during analysis for comparison. Here are links to some discussion that you may find it interesting forum.image.sc/t/how-to-set-threshold-for-image-j-and-then-do-image-calculations/3696 forum.image.sc/t/thresholding-methods-how-to-choose-wisely/25472
@@rahmakhan9740 I am delighted to hear that you enjoyed the IHC videos. Your support means a lot to me. If you have any recommendations for future topics, please let me know. I'm currently making a video on using ImageJ to quantify DAB staining. I will upload it shortly. Please stay tuned, and thank you again for watching.
Thanks for the explanation. One doubt... how do I define thereshold?
You are welcome. The whole point of defining threshold is to select the specific area of interest for analyzing. The image may contain debris or noise that needs to be excluded during thresholding. Performing a correct thresholding is necessary to maintain accuracy of quantification. There is a debate amongst the scientific community, whether to keep the thresholding value same or different across all the images during analysis for comparison. Here are links to some discussion that you may find it interesting forum.image.sc/t/how-to-set-threshold-for-image-j-and-then-do-image-calculations/3696 forum.image.sc/t/thresholding-methods-how-to-choose-wisely/25472
plz do more on ihc quantification.
Thank you. I have plans to include more in the upcoming videos. please stay tuned.
@@nrttaye4033 hello sir, any update on IHC
@@rahmakhan9740 I am delighted to hear that you enjoyed the IHC videos. Your support means a lot to me. If you have any recommendations for future topics, please let me know.
I'm currently making a video on using ImageJ to quantify DAB staining. I will upload it shortly. Please stay tuned, and thank you again for watching.
No problem! I personally find your videos quite helpful, so its great I could make you Happy!
@@nrttaye4033
@@rahmakhan9740 Hello, I am happy to share the IHC quantification for DAB staining. Here is the link th-cam.com/video/K1WKcbKUOMY/w-d-xo.html