Graphical Estimation of Kd from P:L Binding Plot

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  • เผยแพร่เมื่อ 15 ธ.ค. 2024

ความคิดเห็น • 22

  • @santanamendoza4279
    @santanamendoza4279 7 ปีที่แล้ว +23

    I'm very surprised that this video doesn't have more views! By far the best explanation of these graphs I have seen thus far. Thank you!!!

  • @Silent_Sounds
    @Silent_Sounds ปีที่แล้ว

    you are so much better at explaining these concepts than my tenured professor at a nationally recognized research institution. Props and thanks for helping me pass biochem.

  • @tendekaim
    @tendekaim 6 ปีที่แล้ว +3

    omg you save lives! you have no idea...shortest and best explanation ever thank you

  • @0LUKAZ067
    @0LUKAZ067 หลายเดือนก่อน

    Thanks so much for the video, saved me in the last day for my presentation

  • @OrthoMetaParanoid
    @OrthoMetaParanoid 7 ปีที่แล้ว +5

    Brilliant video, thanks!

  • @kal7708
    @kal7708 3 ปีที่แล้ว +1

    This is just amazing! Thanks :)

  • @AmitKSingh-pk3ll
    @AmitKSingh-pk3ll 6 ปีที่แล้ว +1

    very nice explanation

  • @thatboyss5576
    @thatboyss5576 3 ปีที่แล้ว +2

    Would this not give the initial concentration instead of Kd. We would then use this initial concentration to solve for Kd using the equilibrium equation.

  • @shushmagudla3920
    @shushmagudla3920 6 ปีที่แล้ว +1

    So are Km and Kd similar? Km also substrate concentration at 0.5 Vmax. How are the two different?

  • @muhammadkhattak6922
    @muhammadkhattak6922 7 ปีที่แล้ว

    Thanks a lot for the video, you are great at it.This helped me very much with my school stuff. Keep up the good work.

  • @ching-hung-yao
    @ching-hung-yao 7 ปีที่แล้ว +1

    very good video help me a lot

  • @emilyh6442
    @emilyh6442 7 ปีที่แล้ว +1

    So a higher Ka would also mean tighter binding/higher affinity? Thank you

  • @SohanurRahman09
    @SohanurRahman09 5 ปีที่แล้ว +1

    Nice video...Thank you very much.

  • @ummeamara5942
    @ummeamara5942 ปีที่แล้ว

    how to get y-axis value?

  • @curtis4994
    @curtis4994 ปีที่แล้ว

    you're fantastic

  • @atawahperry1852
    @atawahperry1852 6 ปีที่แล้ว

    thanks was useful

  • @stanbaldwin1999
    @stanbaldwin1999 2 หลายเดือนก่อน

    Why use 50%?

  • @busoladaramola9616
    @busoladaramola9616 7 ปีที่แล้ว

    why cant it be at 100% theta, completely saturated ?

    • @robertluxenhofer1579
      @robertluxenhofer1579 5 ปีที่แล้ว +3

      Not using 100% theta has a simple practical reason. As the curve inevitable slowly, asymptotically approaches 100% the 100% is hard to determine experimentally. The 50% value, however, is much more simple to determine.