Lentivirus Transduction Protocol: Infecting your target cells

So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depending on whether you're infecting adherent or suspension cells or difficult-to-transduce cell types, we have different infection recommendations for you! In this video, we'll explain:
→ How to perform a lentiviral transduction experiment
→ An overview of the spinoculation and reverse transduction methods
→ How to confirm transgene expression
Lentiviruses are extremely useful gene delivery systems for delivering cDNA, shRNA, and sgRNA to stably express, knockdown, or knockout your gene of interest in your target cells. Once you learn how to use lentiviruses for your own experiment, you'll be able to target not only dividing cells but also post-mitotic, non-dividing cells like neurons.
→ For more information about lentiviruses, access our free learning resources: info.abmgood.c...
→ Access our full protocol for lentivirus transduction:
www.abmgood.co...
Request a free sample of our ViralEntry™ Transduction Enhancer:
→ www.abmgood.co...
Watch our video "Multiplicity of Infection (MOI): What is it and how do I calculate it?":
→ • Multiplicity of Infect...
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[@jeannguyen4333]

TYSM very interesting for all WW to understand mechanism. Best for new ARN messager WW.

[@abmgood]

Thanks for watching our content!

[@johncgibson4720]

so good

[@Hezekiah890]

Thanks for your video! Do you change the cluture medium into fresh medium without drugs after untransduced cells completely die?

[@cutlerwhitely2269]

Awesome video!

[@abmgood]

Thanks for watching! :)

[@seetheworld6656]

Overnight means how many hour??

[@teteteteta2548]

Cool!

[@saritasenorita7213]

Plz why exactly we need this condition why we care about the CO2?

[@abmgood]

Hello there!
Controlling the temperature and levels of CO2 and humidity in the incubator is critical to the health and growth of cultured cells.
CO2 is needed as part of the media bicarbonate buffering system to regulate its pH. Carbonic acid is formed when water reacts with CO2, which in turn will react with the bicarbonate ions in the media to modulate the pH. The dye Phenol red is commonly used in media to monitor the pH: red indicates a physiological pH, purple shows an alkaline pH and yellow indicates an acidic pH.

[@shakirhussain4096]

@@abmgood
please send me the link to the spinoculation method.

[@HamguyBacon]

What would happen if you Inject a human with a Lentivirus.