DNA microinjection | Embryonic stem cell mediated gene transfer | Gene transfer techniques

[@ethanevansc]

Hey bro … in embryonic stem cell mediated gene transfer, at the final step after injecting the targeted embryonic stem cells into the blastocyst and placing it in a mouse 🐁… the mouse doesn’t give birth to two offsprings with different genetic make up but rather it gives birth to a chimeric mouse. A chimeric organism is composed of cells from two or more genetically distinct individuals.

[@sudhakerketta6578]

My seminar topic is "gene transfer to animal cells" I was so confused about methods... But after watching this... I feel relaxed lil bit🙂 thank you sir 🙏 I hope I can...

[@bioscience6853]

Thank you

[@Akashkumar-jo6mp]

Beautifully explained..!
Thanks hope your channel grow well ..

[@bioscience6853]

My pleasure✨

[@naveenshanmugam9848]

Thank you brother you cleared all my doubts and now i am ready to take the seminar 🥰🥰🥰 keep posting more videos ✌️✌️✌️

[@bioscience6853]

Sure

[@sunkisatheesh1464]

Super explanation sir. Thanq.

[@bioscience6853]

My pleasure

[@belysultana8177]

Useful vedio. That helps to understan the topic clearly

[@bioscience6853]

My pleasure

[@akramrahi3529]

Thank u sir I watch the full video that very clearly I appreciate u sir

[@bioscience6853]

Thank you

[@bandarimahi23]

TQ soo much for your clear explanation ❤ sir

[@AliKhan-ns2dp]

Good but your explanation method is very fast so sorry did not understand on single word

[@madihaali7173]

Amazing explanation

[@vaishnavitomar6001]

Bhai thoda sa aaram se bolo itni jldi kha Jana hai kuch samajh bhi nhi aarha baki Nice explanation 👍

[@shazialone7545]

Sir where is retroviral gene transfer method topic?

[@deepikasripally8839]

Thank you ☺️

[@act_pvt]

Thank you so much

[@bioscience6853]

My pleasure

[@teenasharma7323]

Aap thoda dhere dherre samjhaya karo well done 👍

[@bioscience6853]

Thanks

[@MoviesNepal23]

sir please kindly makes the video about mitochondria and chloroplast gene transformer

[@akramrahi3529]

Next topic transgenic anamils please sir tell me

[@bioscience6853]

Sure

[@abdum6977]

Very nice and simplest explanation 👍👍

[@bioscience6853]

Thanks

[@gayatrihaldar5570]

Thnku so much

[@bioscience6853]

My pleasure

[@hirendabhi7675]

WHICH FACTOR RESPONSIBLE FOR THE NEXT GENERATION WILL TRANSFER INTO GENE TARGETED MICE OR NORMAL MICE.

[@PradeepSharma-ii6jr]

When foreign DNA is transferred to the host cell, how does the foreign DNA bind to the nuclear DNA of the host cell?

[@galmaankhan8338]

I'm a little bit confused. In embryonic stem cell mediated gene transfer method, if the the target genes are incorporated into the ESCs and then added to blastocyst and finally when it is implanted into the host, why do the host has to undergo fertilization? Because after fertilization, the blastocyst will be formed.

[@enjoywithindrajeet891]

Sir aap video rcording kis chij se karate hai bataye please

[@manikgrover9107]

sir plz help me ...about retroviral transfection plz send me link of that vedio of urs

[@saroshsaroshali9654]

Sir explain in hind..and also explain other methods of transformation

[@person.2563]

You make it clear and easy for me🤝
Thank u🤍🤍🤍

[@bioscience6853]

My pleasure

[@syedsafeenanisar7290]

U are awesome bro. Thnks

[@bioscience6853]

☺☺

[@tanmoydas6861]

Can this process used human?

[@TheMinha]

Fan from Kashmir

[@ssjewellers4755]

Thank u sir good lecture

[@bioscience6853]

My pleasure✨

[@Krishna9292p]

sir hindi me explain Karo 😢
nots mast bhanaya hai ❤

[@nothingggg656]

Sirr which vector is used for microinjection plzzz reply

[@shivmunisigh9997]

Thank you sir

[@bioscience6853]

❤☺❤

[@Risky_Rambles]

Thnks

[@bioscience6853]

My pleasure

[@anshikaapatkare]

Sir can we introduce more than one target gene in the ES cells

[@ekhushimanzoor6186]

Nice

[@bioscience6853]

Thanks

[@omerabdullah8556]

kal exma hai isliye yaha aaa k dekhte🤣

[@areebamubeenashraf9571]

In microinjection technique, can we use sperm cell as a host.??After which we fertilize egg cell and the sperm which have foreign DNA. So kindly give me ans...

[@timothy8048]

Thanks yrr

[@kirtisingh46]

Sir ak video hinde me bhi bta de

[@gudipatisridivya7283]

sir please help me with retroviral gene transfer

[@bioscience6853]

Sure

[@Sane_gupta]

Go to 5:07 for embryonic stem cells

[@dimplethakur6447]

Sir what is difference between electroporation and microinjection

[@ritwikbasak4960]

Electrical voltage is used in electroporation for creating pores around cell membrane. And in microinjection you can see it in the video

[@mittapelliramanna1401]

Sir thelugulo cheppandi

[@swapnabehera3451]

Ur words are very speed.
Little difficult to understand 😐

[@saroshsaroshali9654]

Urdu mein ya hindi mein

[@saroshsaroshali9654]

Urdu mein explain krein sir

[@bioscience6853]

I don't know urdu

[@m.s.choudhary4115]

Sir please explain in hindi

[@bioscience6853]

If I explain hindi... South indians can't understand... Hope u understand 🙂

[@m.s.choudhary4115]

@@bioscience6853 but sir
You can make this video in hindi or not ?
We are so many students of rajasthan have subject biotechnology and we are getting right teacher
I feel you are a best teacher so please help us
We will be so much thankful of you

[@mrwrong2893]

You are going to....

[@SonaliRajput2020]

PK

[@AmruMagdy]

Amazing explanation