Total Protein Normalization - Advanced Tutorial

For more information, bio-rad.com/LearnTPN
Learn how to normalize fluorescence western blot signal to total protein using Image Lab.
In this video, we take an in-depth look at how to normalize your multiplex fluorescence western blots to total protein signal. We cover manual adjustment of lanes and bands, correcting for blot background, and how to export the data you need to publish these results.
Want to learn more about the science behind how stain-free technology enables total protein normalization?
Visit us on the web to learn more:
www.bio-rad.com/en-us/applicat...
We Are Bio-Rad.
Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.
Connect with Bio-Rad Online:
Website: www.bio-rad.com/
LinkedIn: / 1613226
Facebook: / biorad
Twitter: / bioradlifesci
Instagram: @BioRadLabs
Snapchat: @BioRadLabs

[@luisp.9710]

Hi. How can I measure the expression level of 3 proteins in the same blot?. I mean, After transfer, I first get the total protein image and once I´ve got the image in the software can I cut the membrane in 3 pieces to incubate each piece of membrane with a primary Ab, my secondary Ab, and then I either at the same time or separately get the image of three proteins (by chemiluminescence) and do the same as this tutorial?. or. it is impossible to cut the membrane and each protein has to be evaluated one by one?.

[@auroraprs9133]

you better to do an immunoblot meaning that your secondary antibodies are conjugated to fluorescence molecules all antibodies are added at the same time too.