I worked as a chemist and as an IT professional with these Beckman counters. We collected the information into data buffers that were like a USB stick in the 1990's. We kept the data on the 9-pin dot matrix paper for backup, but we didn't have to enter them manually in a spreadsheet later. We read the buffer. I'm sure we could come up with a device to pull the serial communication from the counter into a device that would be connected to a nework you could read.
amazing. Just the week I've been trying to learn about how to do liquid scintillation counting on that exact machine - Beckman LS6500. I can't find any videos on how to operate this ancient machine but this was helpful! My only question - how do you recommend I transfer my samples into the scintillation vials? My samples will be coming out of a 96-well plate so ~ 300 uL. Do I just squirt the 300 uL onto the filter paper and then stick that in the tubes containing the scintillation cocktail? Thank you in advance! No one in my lab has done this stuff so I am looking everywhere for guidance. Thanks!
Hi. Happy I could help. That will depend on your sample type. For my assays, I have to wash the filter paper with phosphoric acid to remove free ATP, then acetone to dry. Here's a post with more: bit.ly/kinaseassays Good luck!
Hello , I have a question about dilutions in scintillation liquid. I am measuring H3-tritum release from enzymes in cancer cells and wanted to know what are good sample dilutions for 20mL glass tubes? I bought this liquid just from watching your video but I don’t know how to use it mixing my sample 😅
Hi - I've never worked with H3, or with liquid dilutions for scintillation counting. Sorry! I'm guessing you may need to determine the dilution factors empirically based on the signal you detect. Good luck!
The HP I'm trying to be loves the info and how you explained the concepts :) the RCT I currently am can't focus on anything except that you're touching your print-offs with gloved hands D: (lol, obviously these weren't the gloves you JUST handled radioactive samples with RIGHT?!?) All joking aside, thanks for putting this video out on the internet ❤️
yeah 6500 series got hit with the 2020 bug... it was designed with fore-thought for 2000... but in the simplist way possible.. just changing the roll-over to 2020 instead of 2000... since no one would think it would stlll be used that long. I know I am still surpirsed we are still servicing them.
Hi! Question, would you say your research is more biology or chemistry based? (Or even split or another subject). Also, what do you think is most important for being a successful research scientist? ( other than experience). Thank you!
My research incorporates both, which is what's great about biochemistry! But I don't do synthesis or any "classical" chemistry type things. Instead I make proteins do the work! And I think that it's important to have a passion for it and have good mentors.
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I worked as a chemist and as an IT professional with these Beckman counters. We collected the information into data buffers that were like a USB stick in the 1990's. We kept the data on the 9-pin dot matrix paper for backup, but we didn't have to enter them manually in a spreadsheet later. We read the buffer. I'm sure we could come up with a device to pull the serial communication from the counter into a device that would be connected to a nework you could read.
Was writing my thesis and this video came in clutch! Thank you so much and I appreciate all your videos. They are so awesome!
Thanks so much! So happy I could help. Best of luck with the thesis!
Wow thanks for the informative video. Explained so well. Really helped me understand.
Thanks so much! So glad I could help
amazing. Just the week I've been trying to learn about how to do liquid scintillation counting on that exact machine - Beckman LS6500. I can't find any videos on how to operate this ancient machine but this was helpful! My only question - how do you recommend I transfer my samples into the scintillation vials? My samples will be coming out of a 96-well plate so ~ 300 uL. Do I just squirt the 300 uL onto the filter paper and then stick that in the tubes containing the scintillation cocktail?
Thank you in advance! No one in my lab has done this stuff so I am looking everywhere for guidance. Thanks!
Hi. Happy I could help. That will depend on your sample type. For my assays, I have to wash the filter paper with phosphoric acid to remove free ATP, then acetone to dry. Here's a post with more: bit.ly/kinaseassays
Good luck!
Great explanation, well done 👍
Thank you!
Hello , I have a question about dilutions in scintillation liquid. I am measuring H3-tritum release from enzymes in cancer cells and wanted to know what are good sample dilutions for 20mL glass tubes? I bought this liquid just from watching your video but I don’t know how to use it mixing my sample 😅
Hi - I've never worked with H3, or with liquid dilutions for scintillation counting. Sorry! I'm guessing you may need to determine the dilution factors empirically based on the signal you detect. Good luck!
The HP I'm trying to be loves the info and how you explained the concepts :) the RCT I currently am can't focus on anything except that you're touching your print-offs with gloved hands D: (lol, obviously these weren't the gloves you JUST handled radioactive samples with RIGHT?!?)
All joking aside, thanks for putting this video out on the internet ❤️
not sure what those acronyms are but nothing hot there!! and thank you
yeah 6500 series got hit with the 2020 bug... it was designed with fore-thought for 2000... but in the simplist way possible.. just changing the roll-over to 2020 instead of 2000... since no one would think it would stlll be used that long. I know I am still surpirsed we are still servicing them.
thanks for the insider knowledge. that's really cool that you service them
Hi! Question, would you say your research is more biology or chemistry based? (Or even split or another subject). Also, what do you think is most important for being a successful research scientist? ( other than experience). Thank you!
Love your videos btw!
My research incorporates both, which is what's great about biochemistry! But I don't do synthesis or any "classical" chemistry type things. Instead I make proteins do the work! And I think that it's important to have a passion for it and have good mentors.
thank you!
What are some good quench standards
I am not sure what you mean by "quench standards" sorry!
Hi! What does quench have to do with this?
To stop the reaction and get timepoints