One can also add peroxide to make it harder for mold spores to germinate (obviously only works with mycelium transfers or clones). Combining these would allow only your healthy mycelium to colonize the plate. Make sure to allow the agar to cool below 120 degrees farenheit so the peroxide doesn't disassociate. Also dont add too much. (I forget the right dilution ratio)
Actually Paul Stamets suggested to pour new agar over the bacteria-contaminated old agar but include gentamycin in that new agar. Then of course you have to transfer the mycelium once it colonizes the new agar , away from the bacteria but also from the antibiotics. That’s basically a combination of the methods mentioned in this vid. Unfortunately the Paul never mentioned any ratios of the antibiotics in the agar mix.
Hey did you delete some videos recently. I just watch your mushroom kit review, from shroom supply. I don’t see video anymore..I know it was kind of older but it’s gone can’t find it..
Hey willy how ya been doing lately? Glad to see ur videos again but what are these video episodes u are talkn about? Or is tht the Pateron thing u mentioned?
So if you only need 100mg for 1L of agar and your starting g418 concentration is 100mg/ml with 100ml/bottle you should be able to make 100,000L of AB agar for less than $50 worth of g418.
@katclaus7425 there's 1000 others that explain the same things, a hell of a lot faster and simpler... this dude makes videos for simple-minded 🤡s and 👶s
Literally never had this or any kind of build up on my dishes. I'm rather clean especially for not having a flow hood. I had the nastiest yellow build up looked it up and from what I've seen it's corona. Could be E coli maybe they look fairly similar but it looks like dehydrated piss that's been dried out and was fizzing while drying. So it's bubbly, and a kinda vibrant yellow that's hard to describe. All affected dishes got thrown out so no pics but I mean it caught me off guard a bit. I've never had problems so time for a flow hood I guess. I try to overdue agar anyways just incase I get bad transfers or my jars get nasties from the humidifier. I just redo them when I have to but I'm not doing agar again till I get a flow hood. Lysol spraying a tent used to work so well. Only thing I did different was use a beaker to pour the agar after sterilizing.
@@ironmarkgolf I'm not trying to knock your info but viruses are unable to grow on agar, they need to infect a host cell to replicate. You're probably looking at yeast or some kind of infectious bacteria, similar to when your mucus turns yellow when you're sick
@@ouch2925 idk all I know is when I looked up viruses on agar cause I had never seen this before and usually get 1 or 2 contam out of 30 which to me is really good but the point is I've seen several contams and never seen this yellow build up that looked like if you could burn dehydrated pee. It was bubbled up and very odd yellow
![[UNCUT] The Loyal Pin ปิ่นภักดิ์ EP.15 (1/4)](http://i.ytimg.com/vi/TLBjiHvkKtE/mqdefault.jpg)
5:37
You mean @13:38
Jump to 5:15..... you're welcome
One can also add peroxide to make it harder for mold spores to germinate (obviously only works with mycelium transfers or clones). Combining these would allow only your healthy mycelium to colonize the plate.
Make sure to allow the agar to cool below 120 degrees farenheit so the peroxide doesn't disassociate. Also dont add too much. (I forget the right dilution ratio)
So excited for the next web series episode! The first Episode was **chefs kiss** 🥰🥰🥰💙
What a great testimony. Now I gotta see it!
Actually Paul Stamets suggested to pour new agar over the bacteria-contaminated old agar but include gentamycin in that new agar. Then of course you have to transfer the mycelium once it colonizes the new agar , away from the bacteria but also from the antibiotics.
That’s basically a combination of the methods mentioned in this vid. Unfortunately the Paul never mentioned any ratios of the antibiotics in the agar mix.
I love the traveling series man! You should title it sporehunters! In reference to the old strain hunters series
Starts 5:11
thank you
This Man is here to save the world!! Thank You Willy!! Your invited to my wedding!
When pouring agar over top of healthy mycelium, what temperature do you want your agar to be so you don’t harm the mycelium
Hey did you delete some videos recently. I just watch your mushroom kit review, from shroom supply. I don’t see video anymore..I know it was kind of older but it’s gone can’t find it..
this is 30min video that would normally take 3 min to explain everything
What size Petri dishes do you use?
I've used antibiotic agar to grain with gr8 results
Hey willy how ya been doing lately? Glad to see ur videos again but what are these video episodes u are talkn about? Or is tht the Pateron thing u mentioned?
Thanks for the informative video. Just purchased some dope merch. Thanks fam!
Getamycin sulfate is also used as dogs eye drops
Back with a banger!
How about transfers from wild.
Wats up thanks for the info!
So if you only need 100mg for 1L of agar and your starting g418 concentration is 100mg/ml with 100ml/bottle you should be able to make 100,000L of AB agar for less than $50 worth of g418.
Chlorobutanol treated plates, you are welcome
bro i followed u like 5-6 years ago but haven't seen you in like 3 years, did u change channels? or did TH-cam just unsub me
garlic agar . ive used it for about a year now and i dont get contam. ever. mush luv!
How does one make garlic agar
Yes following
Were did you get the antibiotic?
Glad you back with a video man!
Keep them coming!
Great Content
Good job Willy
Thanks man
Can I put Neosporin in my agar?
Great info, thank you sir.
.can any one suggest any good website were I can get the kit deleverd to India and spores ...plz I need your help
They grow wild in India.
Hey brother. Can you please tell me where I can order a mask like that. I really want one.
I bought jars on Amazon and manure/coco coir that gave me heck. So discouraged.
Yooo, his Instagram is empty?
Is it ok in any way to colonize grain with antibiotic agar?
Please dont consume antibiotics without a reason
It's fine as a one off perhaps but not as a general practice.
OG Willy Myco 👽
Thanks for the great info on antibiotics and mycology. 👏👏👏
Can you show a follow up to slurry tek and methods on preventing contamination using it? The speed up is of interest I'm assuming to allot of people.
as always, skip the first five mintues of the video to get to the actual video. the first five minutes he always spends lengthily plugging himself.
The Info he provides is great and he should get paid for providing it. :)
@katclaus7425 there's 1000 others that explain the same things, a hell of a lot faster and simpler... this dude makes videos for simple-minded 🤡s and 👶s
That what you do when you run a business
19:54 what is the ideal temperature of the hot agar you pour over the set agar?
Please consider getting a lapel mic. I'd love to watch this channel, but I just can't understand you through that mask.
Skip the first 6 minutes
Anybody else having trouble with covid on agar lately?
If your town has an Asian market they most likely sell telephone brand agar for cheap... i pay less than a dollar for a 25 gram packet.
My plates are inch thick in the stuff mate
Literally never had this or any kind of build up on my dishes. I'm rather clean especially for not having a flow hood. I had the nastiest yellow build up looked it up and from what I've seen it's corona. Could be E coli maybe they look fairly similar but it looks like dehydrated piss that's been dried out and was fizzing while drying. So it's bubbly, and a kinda vibrant yellow that's hard to describe. All affected dishes got thrown out so no pics but I mean it caught me off guard a bit. I've never had problems so time for a flow hood I guess. I try to overdue agar anyways just incase I get bad transfers or my jars get nasties from the humidifier. I just redo them when I have to but I'm not doing agar again till I get a flow hood. Lysol spraying a tent used to work so well. Only thing I did different was use a beaker to pour the agar after sterilizing.
@@ironmarkgolf I'm not trying to knock your info but viruses are unable to grow on agar, they need to infect a host cell to replicate. You're probably looking at yeast or some kind of infectious bacteria, similar to when your mucus turns yellow when you're sick
@@ouch2925 idk all I know is when I looked up viruses on agar cause I had never seen this before and usually get 1 or 2 contam out of 30 which to me is really good but the point is I've seen several contams and never seen this yellow build up that looked like if you could burn dehydrated pee. It was bubbled up and very odd yellow
TTF MY Man!
Lol he said tri-ch.. it pronounced tri-k.
bruu you love talking hey holy shit