New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
@@hamiltoncannagar636 If that's the first vaccine I have access to, I would still take it - as I don't have any history of "significant" allergic reactions.
@@immortaltyger1569 I'll take whatever I can get my hands on, tested long term or not!! I'm so afraid of the .03% death rate of rona i'd literally take a new vaccine delivery mechanism right to my DNA. I would roll those dice. Even if it was showing adverse reactions on the first rounds. I'm that afraid
0:55 "CT value was low". I think Vincent probably means his CT value was _high_, indicating that a large number of cycles was required to get to the positive threshold, and hence the amount of virus indicated by the test was low.
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
@@victor_venema This is a quote from the governing body of vaccines in UK. If you want to report that you are so far in your echo chamber it frightens me... This is quite literally a quote from the UK, and a link. Facts are facts, people with severe or serious or significant allergies should NOT be vaccinated with the new Pfizer... at least until more studies are done. Please take the time to read what I share before getting defensive. This is science not politics. Use datapoints.
@@victor_venema You know nothing about me Victor, that couldn't be further from the truth. Anyone interested, the last claim was about the tendency of vaccine recipients getting Bells palsy. I asserted there is a correlation between the Pfizer vax. In a 20,000 person study (20k vaccinated 20k placebo) 4 of the vaccinated group developed Bells (one thankfully got over it) 0 of the placebo group got bells. I falsy claimed that is what snopes said, I misread snopes and for that Victor assumes my lives goals and motivations :x more will die from lockdowns. 3rd world starvation, Harvard study over 100m dead if lockdowns do not end. what predictions of covid show 100m dying?
@@hamiltoncannagar636 I believe here in the UK we're over the worst of it and yet the restrictions are becoming more strict. On one hand the government is imposing these restrictions, on the other hand they're telling everyone they're not working. Hospital admissions and deaths from respiratory infections are at the average level for this time of the year but everywhere is going up a tier everytime they review it. The government and a lot of governments around the world are acting in their personal interests now, here Labour aren't any better they wanted even more restrictions. I wonder after all the vulnerable have been vaccinated what the next excuse to take your rights away will be?
Wouldn't it be great if we had cheap rapid at-home tests for both for those of us who missed the window? having both negative antigen and antibody tests precludes many of us from care.
New info on the posibility of using ORF 8 and ORF 3 for antigen test and also detection of antibodies agaist them on the raise. Hope some one makes them soon
I say it again, where did you get the viral protein from since it has NOT been fully Isolated and subsequently Purified? In other words, what are you EXACTLY testing for Mr Racaniello?
22-1-2021 11:29am Dear Professor , is it possible that due to the limit of detection level of the serological test used was unable to pick up the antibodies ? It is hard to have comparative testing on covid-19 antibodies . Another question : what is the probability of a mucosal spray vaccine for covid-19 ? And an oral vaccine to cover the gut covid-19 levels ? I am not sure about urinary vaccines or ones that target the intestines . Would the Pfizer vaccine (intramuscular injection ) be able to induce and produce mucosal antibodies ? Once the covid-19 manages to lodge itself in the lungs - the reality is that covid-19 may never be eradicated from the body even with vaccinations ? Thank you .
Doc you only know how the test works conceptually. Think about how the test is made and attempt to “conceptually” reverse engineer it. All the antibodies are completely unspecific.
I have high hopes with the new administration making these self administered tests widely available will be a high priority. If everyone at risk just tested twice a week for 2 weeks, then nearly all infectious people would be caught. They isolate for a week until not infectious and dead ends the Ro chain reaction. Also all those who were close to the infectious person could test daily for 14 days before meeting anyone and those infectious people would be caught before they spread it to anyone. Infections would drop 90% every 2 weeks and soon most regions would no longer be at risk.
The problem with testing daily x 14 days is that the current home tests for nasal swabbing costs about $12 per kit (Binax now) AND shipping can take a week before it gets to your home due to supply & demand.
Joyful Stand i got Abbott test kits at Walmart off the shelf. $20 for 2 tests. Results in 15 minutes. If the government made them freely available, we could end this pandemic quickly.
So. All testing is a complete waste of time. All through this talk the one thing that was clear is that there is no certainty in any of these tests. They are all pretty much useless.
the other sad thing is that u needed to state the % of false negatives , because u know anti-science types and anti-maskers will use this to say that ALL the stats and #s are too high ,when in actuality we all know that there are thousands of people who had covid that didn't get tested . I know of at least 5. they didn't get tested because they were afraid of how it would affect their health insurance (another pitiful amerikan failure )
It is unfortunate our authorities have not made developing this type of test a real priority. Mass at-home testing could go far toward reducing the spread of infection and making our schools and businesses able to return to more normal operations.
It's Dr. Mina from Harvard that has been championing the idea that as a "public health" instrument (not a "medical" instrument), these rapid paper antigen tests should be approved. If the FDA were a person, I'd say they needed to be charged with manslaughter for holding back this technology from use. Thousands of people have died needlessly because they didn't/don't allow tests that pick-up high viral load (Ct measures), instead, choosing to compare it to PCR tests directly (sensitivity, specificity) without taking into account that the PCR tests take time...time when the person is spreading the disease.
@@sengsational Have you seen press conf.12-4 Houston: th-cam.com/video/4V3yxrJwJQs/w-d-xo.html ICU MDs present evidence for IVERMECTIN to prevent& treat C. Frontline Covid Critical Care Alliance. I take to prevent w/o side effects. Great safety since 80's.
I was told the problem with the antibody test is that it’s not very sensitive and if you had a CoVid infection more than a month ago it won’t work. It seems like it’s in the interest of the panic crowd to have an overly sensitive PCR test and a low sensitivity anti - body test.
I personally know 6 people who tested positive. All had these things in common -100% asymptomatic -no sickness during 2020 -no residents of their households became ill during home-isolation. In fact I know of no one who've had any form of influenza during 2020, including myself. I'm from Denmark and we actually count total number of deaths per year, and have done so for a long time. 2020 has been a year of very low mortality, compared to previous years, there is no sign of a dangerous pandemic. Yet we're locking down yet again because of these bogus numbers given by pcr tests. In 2021 and ongoing the consequences will start to show, we've stopped screening and diagnosing cancer and a broad range of other diseases and conditions, and people will inevitably start dying from easily treatable diseases, so it should be expected that the death rates starts climbing. Also now when a majority of the population suddenly decides to take a syringe which is not a vaccine, this is mRNA gene-therapy (cov-vacination) So that should also cause a huge amount of unexpected death and illness. This will all be attributed to covid19 virus, which will cause even more forced vaccination, forced isolation, and a true pandemic unfolds.
You may have had low mortality because of the lockdown, Denmark certainly seems to have had less than neighboring Sweden which had no lockdown. My own feeling is that the lives saved during the first wave will be lost during this winter. And there could be other reasons for Sweden's high mortality, after all Malmo took about the same hit as Copenhagen, suggests to me that it's geography, not politics that's driving this thing.
@@colinmacdonald5732 you seem to miss my point entirely, the mortality rate in Denmark as a whole was lower during a pandemic than it was in a time with no pandemic. The obvious conclusion is that there isn't a pandemic. But as they say, the only way to stop this 'pandemic' is to turn off your TV, since this is the only place where the pandemic exists.
@@Lasse3 Hope you're right! On the other hand I sorta want the pandemic to be real, otherwise we're most stupid generation in history, we crashed the economy for nothing.
What is the cost of this test? Can this be used for home test since it only takes 10 mins tor read and no lab?whould this be a better system because one could reset yourself all the time?
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
So you confirmed the PCR was wrong, by doing an antibody test? Clearly 1 test was wrong, but you cannot possibly know which one. You actually stated at the start, you were sent the antibody test, by a company DEVELOPING the kit.
People may soon be required to submit to weekly PCR tests, to keep their jobs. Researchers claim that AGnp's (silver nanoparticles) inhibit DNA replication. Question: Can a nasal spray solution of ionic silver and AGnp, administered before a nasal swab sample is taken, defeat a deliberately inflated CT-PCR test?
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
Where can you buy at home antibody tests annonomously? I have been looking into this for months. I can't find a place that does not require your personal information. I just want an annonomous antibody test? But there isn't one available that's odd I think... Kind of makes it seem like this whole covid thing is all about collecting people's info and sending results to govt when you can't just buy one annonomously.
Since symptoms of an infection are caused by your body fighting that infection; wouldn't "asymptomatic infection" mean you are going to die because an infection is progressing without a response to keep it in check? Is there a mechanism for a viral infection to suddenly disappear without your body fighting it off?
Unless something very recently changed, these lateral flow tests (although seemingly “point-of-care” or DYI) are actually to supposed be done and interpreted by CLIA licensed labs under the FDA EUA’s so that those labs can collect validation data and report back to the FDA. We were very excited about using them early on in the pandemic as a surrogate for detecting exposure/ immunity when PCR testing wasn’t as readily available for everyone, but their real-world reliability proved to be all over the map depending on the manufacturer. Some claiming a whole blood drop like you used was not adequate and had to be a serum sample for accuracy. Also, they don’t specifically test for neutralizing antibodies and have cross-reactivity with non-SARS-coV-2 coronaviruses. Maybe Dr Griffin and others can help clarify some of these points? Always appreciate your intelligent discussions!!
@@loveforsberg530 I think because pregnancy tests were not released in a hurry in response to a deadly pandemic. They are simple but went through usual regulatory measures. There were hundreds of companies outside of the US releasing Covid serology tests without any vetting except by the company themselves... then FDA stepped in appropriately. It was like the Wild West.
If you use the tests as a *public health* tool, it doesn't matter much if the results are "perfect". The spit or snot test that's being championed by Dr. Mina will keep the worst offenders (spreaders) out of circulation. That's all we want out of those tests. Don't confuse a "medical" test with a "public health" test. Two different things. Even a cave man can understand that, but unfortunately, the FDA has the IQ of less than a cave man.
Your CT was 55? At that level you’ll be picking up anything , the mucosa could have had any number of residuals from other viruses even a few common cold would set it off . That’s the problem when you start testing at that level with a pcr and non symptomatic it’s a shot in the dark or if you like throwing a dart blindfolded you could hit anything .
Just a peagogical note it would have helped me to "see" the process with a table or tables with the names. It is tricky to hold the anti anti whaterer names in your head if you are not already acustomed to them. Great video on the mechanism though.
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
Mille grazie, Don Dottore Professore Vincenzo! Thanks for yet another clear and edifying presentation. How many of these excellent videos before I can tack a little virology badge onto my French degree?
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
Very nice presentation, sir. I was praying that you would discover antibodies in your blood sample. Hopefully you will soon be vaccinated and will develop immunity to disease. Thank you for the informative solo podcast. Well done.
Considerations: 1)Some people doesn´t produce antibodies at all (2-10%) 2)Those tests are not sensitive enough 3) it may take up to 3 weeks to produce antibodies... Have you done a posterior test? Excelent explanation of lateral flow tests! Thanks for sharing your knowledge! I watch almost everything you do... :D
It was a good description as to how it works. This query is sort of related to another comment below. What would the result mean if the test was too far into the future after a ‘real’ infection? If it is ‘negative’, does it just mean that one no longer has the relative antibodies? Is there a reasonable timeframe from a noticeable infection of some sort for it to produce a meaningful result? Is it true that it can’t demonstrate whether one is immune, or not to anything - except within a narrow period of time?
Thanks for doing this, Vincent. But I think a lot of us (including your cohosts, based on what they have said in previous episodes) were hoping you would do Ian Lipkin as a positive control! Still, thank you for creating this great educational resource for people to understand what is going on.
sounds way better then the nasual swap things they often do. it also looks that due to it being blood, and it circulating the whole body, that the place where the infection currently might be, is irrelevant to the result. fo you perhaps know ehats the rate of false results?
how Many innocent rabbits have been killed . Humans are an awful species. animals arent here for humans to use and abuse , I guess u all dont know that yet
seems simple enough, yet the confusion is never investigated.. New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet+
Why would you think it would vary by test? Do you mean to say, "How long might we expect IgG antibodies to last?" Perhaps I don't understand your question.
@@Idontwannahandl Antibodies are either present or they are not. As we know, they appear to wane after several months. Why do you ask if this test might only detect them for a limited period of time?
I'm curious about test sensitivity. I assume that the test can detect antibodies in the early post-infection phase when they are highest but I'm wondering about later time points (1-2 years post infection, for example). I know that detecting antibodies has been a challenge with SarsCov2, even in people who had confirmed infections.
Very good question! He did four total tests, 2 at the testing place for the PCR test, which had conflicting results, and the antibody tests both showed he was not infected. I think it's all about probability, not absolute certainty. It would be interesting to see the math behind the probability of infection based on all those test results but the accuracy of the particular tests may not be well defined, especially the antibody test which was still in development.
Professor, that is what I call informative and precise information that ordinary men or women can understand from a virologist and other scientist like Dr. Sucharit Bhakdi from his vlog ARE WE BEING THE TOLD THE TRUTH ABOUT COVID-19.
Studies have shown that the sensitivity of the rapid tests are higher when using plasma/serum. Draw 100 ul blood from your finger palp and let it sit for about 2 hours. Draw 20 ul of the serum and repeat the test. Whole blood resulted in a negative test for me, repeating the test with serum showed a positive IgG result. Confirmed now with ELISA.
One of the things I found fascinating, and perhaps it's happening in other less-studied viruses: quite a few people have gotten IgG *before* IgM for SARS-CoV-2. There was a fairly early paper from China studying factors that led to re-hospitalization, and that was one common quirk. It blew me away because I'd always heard IgG comes after IgM, but, as the old joke goes, “The immune system is very complicated ….”
How much would they cost..??having reliable serum covid 19 testing would save a lot of money versus nasal swab plus more accuracy .thats why theyre not priority..cash registers have to sing.
Thank God there are people like you that believe in real valid and reliable science. I have been sending links of your TWiV to all my colleagues and friends trying to minimize the affects that are driven by fear and misinformation. Likewise to Daniel Griffin. I will sending some $ soon. Dr M J Wine
That's basically like what you have to do for a blood glucose test except you also have to add the buffer. What I am curious about is whether people who were infected might make antibodies to other surface proteins and not to the spike. Maybe a person would always make antibodies to all the surface proteins but since there are several, I do wonder if it would even be possible for someone to make antibodies to one or more than aren't the spike protein.
Is it possible that the PCR had picked up sars cov-2 but that your mucosal and t cell response was adequate enough so that you didn't need antibody response that would be detected?
Not the blood test, but the nose test....I'm not sure this is legit, but about $10 each in a box of 25: store.genprice.com/sars-cov-2/panbio-covid-19-ag-rapid-test-device/
Hi Vincent. Thank you for this video and explanation. Just correct the scheme because What you show in this assay as the control conjugates is not right. The control line should react with the SAME conjugates as the test line, otherwise this is not a real control.
Is there a test that I could take at home to test for measles antibodies or mumps or Epstein Barr perhaps.. or tetanus? Or do I have to go to a pathologist to get blood drawn?
Thank you so much for bringing the science to those of us who otherwise would have to depend on the news for information on sars CO v 2. I have learned so much from watching twiv and your other podcasts. I have shared this knowledge with so many oeoe people who Are misinformed via news and a multitude of sources of misinformation
Thnx for the video... even though I am a scientist.. it can be hard to explain this to family members who, rightfully, feel completely lost when it comes to understanding immunoresponse, etc. It might be worth taking the time to make second video and peg your audience to elementary or junior high school level (no, seriously). I suspect most adults will find that more accessible AND it will become a resource for education. Finally, and as I am sure you are tired of doing so by now, its worth the time to reiterate that a negative test on either PCR or Ab tests is just a snapshot in time and not an indication of one's infection status after the test nor an indication of one being IMMUNE to SARS-CoV-2. I wonder how many ppl think that a positive Ab (IgM or IgG) indicates an enduring immunity (when we know that story is still poorly understood at the moment)
Hansen et all perhaps ZDoggMD have that covered rather well; Dare thee not discuss *[MONKEY]* & how these are portrayed in Dorothy Wizard of Oz prior to all this; Question is sero positive correctly *Ab++* or just a single "+" *Ab+* I have some fringe work-if you are interested you can post on some of my YT videos then for what you say I have seen accomplished work from under 18 which is very very informed at this level here
I'm asking my PCP to order this test on Monday. Insurance pays 100%. Not FDA approved, only EUA..so should not inform any decisions re:health. Mostly just to satisfy my curiosity.
Great explanation for how this type of test works. Thanks!
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
@@hamiltoncannagar636 If that's the first vaccine I have access to, I would still take it - as I don't have any history of "significant" allergic reactions.
@@immortaltyger1569 I'll take whatever I can get my hands on, tested long term or not!! I'm so afraid of the .03% death rate of rona i'd literally take a new vaccine delivery mechanism right to my DNA. I would roll those dice.
Even if it was showing adverse reactions on the first rounds.
I'm that afraid
0:55 "CT value was low". I think Vincent probably means his CT value was _high_, indicating that a large number of cycles was required to get to the positive threshold, and hence the amount of virus indicated by the test was low.
Exactly, as he explains in the linked video.
yeah that threw me but then I see this as the top comment - got scared for a second that I had the PCR test all wrong
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
Are you looking for a gift for your significant other and can't decide between jewellry and something useful? Give them laterlal flow assays!
(Not spam, just my bad attempt at humor with all the gold particles).
@@loveforsberg530 Santa says, "Ho Ho Ho for lateral flow."
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
An antibody test is the most useful gift anyone could give me at the moment and the gold is an added bonus. Had the PCR more than 2 weeks too late.
He Went To Rabbit!
I'll let you know what Quest bills insurance next week. Retail cost is like $120. I'm imagining they bill insurance $2000 lol
💔💔💔💔💔panda Meixiang had been abused for years in washington zoo💔💔💔💔💔
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
@@victor_venema This is a quote from the governing body of vaccines in UK. If you want to report that you are so far in your echo chamber it frightens me... This is quite literally a quote from the UK, and a link.
Facts are facts, people with severe or serious or significant allergies should NOT be vaccinated with the new Pfizer... at least until more studies are done. Please take the time to read what I share before getting defensive. This is science not politics. Use datapoints.
@@victor_venema You know nothing about me Victor, that couldn't be further from the truth. Anyone interested, the last claim was about the tendency of vaccine recipients getting Bells palsy. I asserted there is a correlation between the Pfizer vax. In a 20,000 person study (20k vaccinated 20k placebo) 4 of the vaccinated group developed Bells (one thankfully got over it) 0 of the placebo group got bells.
I falsy claimed that is what snopes said, I misread snopes and for that Victor assumes my lives goals and motivations :x
more will die from lockdowns. 3rd world starvation, Harvard study over 100m dead if lockdowns do not end.
what predictions of covid show 100m dying?
@@hamiltoncannagar636 I believe here in the UK we're over the worst of it and yet the restrictions are becoming more strict. On one hand the government is imposing these restrictions, on the other hand they're telling everyone they're not working. Hospital admissions and deaths from respiratory infections are at the average level for this time of the year but everywhere is going up a tier everytime they review it. The government and a lot of governments around the world are acting in their personal interests now, here Labour aren't any better they wanted even more restrictions. I wonder after all the vulnerable have been vaccinated what the next excuse to take your rights away will be?
Thanks for teaching us. I’m waiting for my igG results.
Bro if c Result is positive right ?
Very interesting and worthwhile video.
Wow! Thank you so much, Vincent. I'm glad you're negative.
Why? That makes no sense at all. If he has a positive antibody test it means he 's immune and will not need the vaccine.
Wouldn't it be great if we had cheap rapid at-home tests for both for those of us who missed the window? having both negative antigen and antibody tests precludes many of us from care.
Thanks. I wonder about the specificity of lateral flow for COVID
Can you do a video on the Delta variant?
New info on the posibility of using ORF 8 and ORF 3 for antigen test and also detection of antibodies agaist them on the raise. Hope some one makes them soon
It's December 2021 and these at home antibody tests are still unobtanium.
I say it again, where did you get the viral protein from since it has NOT been fully Isolated and subsequently Purified?
In other words, what are you EXACTLY testing for Mr Racaniello?
thank you Professor greetings from Venezuela. Love your colored keyboard y the back
22-1-2021 11:29am Dear Professor , is it possible that due to the limit of detection level of the serological test used was unable to pick up the antibodies ? It is hard to have comparative testing on covid-19 antibodies . Another question : what is the probability of a mucosal spray vaccine for covid-19 ? And an oral vaccine to cover the gut covid-19 levels ? I am not sure about urinary vaccines or ones that target the intestines . Would the Pfizer vaccine (intramuscular injection ) be able to induce and produce mucosal antibodies ? Once the covid-19 manages to lodge itself in the lungs - the reality is that covid-19 may never be eradicated from the body even with vaccinations ? Thank you .
Doc you only know how the test works conceptually. Think about how the test is made and attempt to “conceptually” reverse engineer it. All the antibodies are completely unspecific.
So. PCR tests personally described to me by knowledgeable person.
100% false positives!
are those alcohol pads? weren't you supposed to clean your finger with that first? *eyeball emojie*
You mean your CT was high?
I have high hopes with the new administration making these self administered tests widely available will be a high priority. If everyone at risk just tested twice a week for 2 weeks, then nearly all infectious people would be caught. They isolate for a week until not infectious and dead ends the Ro chain reaction. Also all those who were close to the infectious person could test daily for 14 days before meeting anyone and those infectious people would be caught before they spread it to anyone. Infections would drop 90% every 2 weeks and soon most regions would no longer be at risk.
The problem with testing daily x 14 days is that the current home tests for nasal swabbing costs about $12 per kit (Binax now) AND shipping can take a week before it gets to your home due to supply & demand.
Joyful Stand i got Abbott test kits at Walmart off the shelf. $20 for 2 tests. Results in 15 minutes. If the government made them freely available, we could end this pandemic quickly.
False positive? How is it possible?
So. All testing is a complete waste of time. All through this talk the one thing that was clear is that there is no certainty in any of these tests. They are all pretty much useless.
the other sad thing is that u needed to state the % of false negatives , because u know anti-science types and anti-maskers will use this to say that ALL the stats and #s are too high ,when in actuality we all know that there are thousands of people who had covid that didn't get tested . I know of at least 5. they didn't get tested because they were afraid of how it would affect their health insurance (another pitiful amerikan failure )
Be careful 🥰
sorry, dr, but I have to borrow some places from your comment area: 💔💔💔💔💔panda Meixiang had been abused for years
in washington zoo💔💔💔💔💔
If you're sorry, then stop doing it. Then you can stop being sorry and let your conscience rest easier.
It is unfortunate our authorities have not made developing this type of test a real priority. Mass at-home testing could go far toward reducing the spread of infection and making our schools and businesses able to return to more normal operations.
I believe they have been developed but are not yet approved. I'm thinking of Dr. Mena from Harvard who has appeared on TWIV and Medcram several times.
It's Dr. Mina from Harvard that has been championing the idea that as a "public health" instrument (not a "medical" instrument), these rapid paper antigen tests should be approved. If the FDA were a person, I'd say they needed to be charged with manslaughter for holding back this technology from use. Thousands of people have died needlessly because they didn't/don't allow tests that pick-up high viral load (Ct measures), instead, choosing to compare it to PCR tests directly (sensitivity, specificity) without taking into account that the PCR tests take time...time when the person is spreading the disease.
@@sengsational Have you seen press conf.12-4 Houston: th-cam.com/video/4V3yxrJwJQs/w-d-xo.html ICU MDs present evidence for IVERMECTIN to prevent& treat C. Frontline Covid Critical Care Alliance. I take to prevent w/o side effects. Great safety since 80's.
I was told the problem with the antibody test is that it’s not very sensitive and if you had a CoVid infection more than a month ago it won’t work. It seems like it’s in the interest of the panic crowd to have an overly sensitive PCR test and a low sensitivity anti - body test.
A great explanation of how lateral flow assays work in general and this test in particular. Thank you, Vincent!
Damning to the PCR test. How many false positives are out there?
I personally know 6 people who tested positive.
All had these things in common
-100% asymptomatic
-no sickness during 2020
-no residents of their households became ill during home-isolation.
In fact I know of no one who've had any form of influenza during 2020, including myself.
I'm from Denmark and we actually count total number of deaths per year, and have done so for a long time.
2020 has been a year of very low mortality, compared to previous years, there is no sign of a dangerous pandemic. Yet we're locking down yet again because of these bogus numbers given by pcr tests.
In 2021 and ongoing the consequences will start to show, we've stopped screening and diagnosing cancer and a broad range of other diseases and conditions, and people will inevitably start dying from easily treatable diseases, so it should be expected that the death rates starts climbing.
Also now when a majority of the population suddenly decides to take a syringe which is not a vaccine, this is mRNA gene-therapy (cov-vacination)
So that should also cause a huge amount of unexpected death and illness. This will all be attributed to covid19 virus, which will cause even more forced vaccination, forced isolation, and a true pandemic unfolds.
@@Lasse3 I hear you.
You may have had low mortality because of the lockdown, Denmark certainly seems to have had less than neighboring Sweden which had no lockdown. My own feeling is that the lives saved during the first wave will be lost during this winter. And there could be other reasons for Sweden's high mortality, after all Malmo took about the same hit as Copenhagen, suggests to me that it's geography, not politics that's driving this thing.
@@colinmacdonald5732 you seem to miss my point entirely, the mortality rate in Denmark as a whole was lower during a pandemic than it was in a time with no pandemic.
The obvious conclusion is that there isn't a pandemic. But as they say, the only way to stop this 'pandemic' is to turn off your TV, since this is the only place where the pandemic exists.
@@Lasse3 Hope you're right! On the other hand I sorta want the pandemic to be real, otherwise we're most stupid generation in history, we crashed the economy for nothing.
A positive PCR test is actually good news. That’s the route to natural immunity.
What is the cost of this test? Can this be used for home test since it only takes 10 mins tor read and no lab?whould this be a better system because one could reset yourself all the time?
I would do the test twice every time.
$ 24 for two tests . Kroger sells them for that
I'll say it again. Vincent is a national treasure.
Viruses are not contagious.
@@Stefan-gh7xr I'm not willing to play. Have a nice holiday.
This is the best simple to understand guide to how the basics of the anti-body timeline I have seen.
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
So you confirmed the PCR was wrong, by doing an antibody test?
Clearly 1 test was wrong, but you cannot possibly know which one.
You actually stated at the start, you were sent the antibody test, by a company DEVELOPING the kit.
People may soon be required to submit to weekly PCR tests, to keep their jobs. Researchers claim that AGnp's (silver nanoparticles) inhibit DNA replication. Question: Can a nasal spray solution of ionic silver and AGnp, administered before a nasal swab sample is taken, defeat a deliberately inflated CT-PCR test?
This was a wonderful teaching video, I'll be sending it along to friends and family confused about the whole idea of testing.
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
Can all covid antibody tests show positive if you’re just positive for having had the common cold? That’s what they said on 60 Minutes.
Where can you buy at home antibody tests annonomously?
I have been looking into this for months. I can't find a place that does not require your personal information.
I just want an annonomous antibody test? But there isn't one available that's odd I think... Kind of makes it seem like this whole covid thing is all about collecting people's info and sending results to govt when you can't just buy one annonomously.
Since symptoms of an infection are caused by your body fighting that infection; wouldn't "asymptomatic infection" mean you are going to die because an infection is progressing without a response to keep it in check? Is there a mechanism for a viral infection to suddenly disappear without your body fighting it off?
Unless something very recently changed, these lateral flow tests (although seemingly “point-of-care” or DYI) are actually to supposed be done and interpreted by CLIA licensed labs under the FDA EUA’s so that those labs can collect validation data and report back to the FDA. We were very excited about using them early on in the pandemic as a surrogate for detecting exposure/ immunity when PCR testing wasn’t as readily available for everyone, but their real-world reliability proved to be all over the map depending on the manufacturer. Some claiming a whole blood drop like you used was not adequate and had to be a serum sample for accuracy. Also, they don’t specifically test for neutralizing antibodies and have cross-reactivity with non-SARS-coV-2 coronaviruses. Maybe Dr Griffin and others can help clarify some of these points? Always appreciate your intelligent discussions!!
Pregnancy tests are routinely done at home with no issues. It is highly regulated, but there is no reason it should be.
@@loveforsberg530 I think because pregnancy tests were not released in a hurry in response to a deadly pandemic. They are simple but went through usual regulatory measures. There were hundreds of companies outside of the US releasing Covid serology tests without any vetting except by the company themselves... then FDA stepped in appropriately. It was like the Wild West.
It could mean you’re a very sick rabbit.
The question "did the rabbit die?" used to have an important meaning
Thank you for the clear explanation Dr. R.
The sooner the home rapid antigen and antibody tests are available the better.
What's the true positive & true negative? Isn't it about time to move past EUA?
@stephanie h, yes, that was what we were saying 7 months ago. USA - not exactly fast off the start.
If you use the tests as a *public health* tool, it doesn't matter much if the results are "perfect". The spit or snot test that's being championed by Dr. Mina will keep the worst offenders (spreaders) out of circulation. That's all we want out of those tests. Don't confuse a "medical" test with a "public health" test. Two different things. Even a cave man can understand that, but unfortunately, the FDA has the IQ of less than a cave man.
Your CT was 55? At that level you’ll be picking up anything , the mucosa could have had any number of residuals from other viruses even a few common cold would set it off . That’s the problem when you start testing at that level with a pcr and non symptomatic it’s a shot in the dark or if you like throwing a dart blindfolded you could hit anything .
Just a peagogical note it would have helped me to "see" the process with a table or tables with the names. It is tricky to hold the anti anti whaterer names in your head if you are not already acustomed to them. Great video on the mechanism though.
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
Mille grazie, Don Dottore Professore Vincenzo! Thanks for yet another clear and edifying presentation. How many of these excellent videos before I can tack a little virology badge onto my French degree?
Thanks, Earth's ViroProf,
you are a touchstone for us👍 a true teaching talent....
(My bio/chem bkgrd was 40+ years ago🤣)
New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet
Asymptomatic means you're not sick. That is not an infection.
Thank you !
As a layman, I have gained a much better knowledge and understanding of Covid-19 by listening to Mr. Racaniello's podcasts.
This is complementary: th-cam.com/video/ROfUYTcagVE/w-d-xo.html
The Best Earth's Virology Professor! (in my humble opinion)
Very nice presentation, sir. I was praying that you would discover antibodies in your blood sample. Hopefully you will soon be vaccinated and will develop immunity to disease. Thank you for the informative solo podcast. Well done.
Considerations: 1)Some people doesn´t produce antibodies at all (2-10%) 2)Those tests are not sensitive enough 3) it may take up to 3 weeks to produce antibodies... Have you done a posterior test? Excelent explanation of lateral flow tests!
Thanks for sharing your knowledge! I watch almost everything you do... :D
It was a good description as to how it works. This query is sort of related to another comment below. What would the result mean if the test was too far into the future after a ‘real’ infection? If it is ‘negative’, does it just mean that one no longer has the relative antibodies? Is there a reasonable timeframe from a noticeable infection of some sort for it to produce a meaningful result? Is it true that it can’t demonstrate whether one is immune, or not to anything - except within a narrow period of time?
I've wondered if we will be taking antibody tests after getting the vaccine since we don't know how long we'll be immune?
Thanks for doing this, Vincent. But I think a lot of us (including your cohosts, based on what they have said in previous episodes) were hoping you would do Ian Lipkin as a positive control! Still, thank you for creating this great educational resource for people to understand what is going on.
Thank you, that was very informative!
sounds way better then the nasual swap things they often do. it also looks that due to it being blood, and it circulating the whole body, that the place where the infection currently might be, is irrelevant to the result.
fo you perhaps know ehats the rate of false results?
how Many innocent rabbits have been killed . Humans are an awful species. animals arent here for humans to use and abuse , I guess u all dont know that yet
Thanks Vincent. Very well explained
Do a test on a person who should have the antibodies
seems simple enough, yet the confusion is never investigated.. New discovery: The governing body of vaccines in the UK has urged people who have a history of "significant" allergic reactions not to have the Pfizer/BioNtech COVID-19 vaccine. www.euronews.com/2020/12/09/pfizer-vaccine-uk-body-tells-people-with-history-of-allergic-reactions-not-to-get-jab-yet+
Yes, these tests are easy to do as long as you are fine and not feverish. Stay safe and healthy !
How long might we expect the IgG antibodies to be detectable by a test like this one?
Why would you think it would vary by test? Do you mean to say, "How long might we expect IgG antibodies to last?" Perhaps I don't understand your question.
@@david.juillet yup. Think you don't understand lol
💔💔💔💔💔panda Meixiang had been abused for years in washington zoo💔💔💔💔💔
@@Idontwannahandl Antibodies are either present or they are not. As we know, they appear to wane after several months. Why do you ask if this test might only detect them for a limited period of time?
I'm curious about test sensitivity. I assume that the test can detect antibodies in the early post-infection phase when they are highest but I'm wondering about later time points (1-2 years post infection, for example). I know that detecting antibodies has been a challenge with SarsCov2, even in people who had confirmed infections.
Hope ur rapid antibody test kit is not made in China .
False positive. How do you know. Just because rapid came back negative? How do you prove ?
Very good question! He did four total tests, 2 at the testing place for the PCR test, which had conflicting results, and the antibody tests both showed he was not infected. I think it's all about probability, not absolute certainty. It would be interesting to see the math behind the probability of infection based on all those test results but the accuracy of the particular tests may not be well defined, especially the antibody test which was still in development.
Professor, that is what I call informative and precise information that ordinary men or women can understand from a virologist and other scientist like Dr. Sucharit Bhakdi from his vlog ARE WE BEING THE TOLD THE TRUTH ABOUT COVID-19.
Hey doc. All the tests are useless. Have a good one.
Studies have shown that the sensitivity of the rapid tests are higher when using plasma/serum. Draw 100 ul blood from your finger palp and let it sit for about 2 hours. Draw 20 ul of the serum and repeat the test. Whole blood resulted in a negative test for me, repeating the test with serum showed a positive IgG result. Confirmed now with ELISA.
Very good info. Thanks for sharing
One of the things I found fascinating, and perhaps it's happening in other less-studied viruses: quite a few people have gotten IgG *before* IgM for SARS-CoV-2. There was a fairly early paper from China studying factors that led to re-hospitalization, and that was one common quirk. It blew me away because I'd always heard IgG comes after IgM, but, as the old joke goes, “The immune system is very complicated ….”
How much would they cost..??having reliable serum covid 19 testing would save a lot of money versus nasal swab plus more accuracy .thats why theyre not priority..cash registers have to sing.
Thank God there are people like you that believe in real valid and reliable science. I have been sending links of your TWiV to all my colleagues and friends trying to minimize the affects that are driven by fear and misinformation. Likewise to Daniel Griffin. I will sending some $ soon. Dr M J Wine
That's basically like what you have to do for a blood glucose test except you also have to add the buffer. What I am curious about is whether people who were infected might make antibodies to other surface proteins and not to the spike. Maybe a person would always make antibodies to all the surface proteins but since there are several, I do wonder if it would even be possible for someone to make antibodies to one or more than aren't the spike protein.
Is it possible that the PCR had picked up sars cov-2 but that your mucosal and t cell response was adequate enough so that you didn't need antibody response that would be detected?
Study after study are showing that these tests are at best 50% effective. Sorry guys, this technology is still 6+ months away.
i've seen on other videos that they dropped the blood on the square well and the buffer on the oval well, so which one is correct?
Where in the USA did you/can we get these antibody tests?
Listen to the video this isn’t approved yet so you can’t get it for the public. Like every thing in this pandemic sit at home and wait. :(
Not the blood test, but the nose test....I'm not sure this is legit, but about $10 each in a box of 25: store.genprice.com/sars-cov-2/panbio-covid-19-ag-rapid-test-device/
Hi Vincent. Thank you for this video and explanation. Just correct the scheme because What you show in this assay as the control conjugates is not right. The control line should react with the SAME conjugates as the test line, otherwise this is not a real control.
What about that some people do not generate antibodies vs SARS-CoV-2 anyway?
Vincent, you should have stated how long it takes for the assay to finish the test. Was it minutes, hours, days...what?
Is there a test that I could take at home to test for measles antibodies or mumps or Epstein Barr perhaps.. or tetanus? Or do I have to go to a pathologist to get blood drawn?
The fast feet oddly spot because unit ontogenetically confess round a ugliest transmission. selfish, curious odometer
This company you mention has an antibody covid test available for individuals to do at home where is that available or what is the company name
How does the gold work?
Thank you so much for bringing the science to those of us who otherwise would have to depend on the news for information on sars CO v 2. I have learned so much from watching twiv and your other podcasts. I have shared this knowledge with so many oeoe people who Are misinformed via news and a multitude of sources of misinformation
It means you should wait for JNJ. One shot deal. Best technology.
Feeling pretty certain you precede an available criticism of population incidence statistics vs bats and sci fi labs professor
Is it possible to have the virus and fight it off prior to an antibody response?
Thnx for the video... even though I am a scientist.. it can be hard to explain this to family members who, rightfully, feel completely lost when it comes to understanding immunoresponse, etc. It might be worth taking the time to make second video and peg your audience to elementary or junior high school level (no, seriously). I suspect most adults will find that more accessible AND it will become a resource for education. Finally, and as I am sure you are tired of doing so by now, its worth the time to reiterate that a negative test on either PCR or Ab tests is just a snapshot in time and not an indication of one's infection status after the test nor an indication of one being IMMUNE to SARS-CoV-2. I wonder how many ppl think that a positive Ab (IgM or IgG) indicates an enduring immunity (when we know that story is still poorly understood at the moment)
Hansen et all perhaps ZDoggMD have that covered rather well; Dare thee not discuss
*[MONKEY]* & how these are portrayed in Dorothy Wizard of Oz prior to all this;
Question is sero positive correctly *Ab++* or just a single "+" *Ab+*
I have some fringe work-if you are interested you can post on some of my YT videos then for what you say I have seen accomplished work from under 18 which is very very informed at this level here
Nice Professor
Very interesting. Thanks. I understand that some people who get COVID don't have an anti-body response. Perhaps you could talk about how that works.
It's called they have another virus. Pcr can detect a virus. It can't tell you what that virus is. It definitely can not detect disease.
Thank you for a very valuable video. I 'll continue learning from you
Rocking the Ronald Jenkees intro/outro?
how long after having contracted covid do the antibodies last?
What if you put rabbit blood on the assay?
Very well explained 👍 Thanks
I have been getting something from Quest it is supposed a test of some sort for covid, it cost 120$
I'm asking my PCP to order this test on Monday. Insurance pays 100%. Not FDA approved, only EUA..so should not inform any decisions re:health. Mostly just to satisfy my curiosity.
Not much harder than a BGC test
IgM or IgG antibodies are very common in the general population and not specific to Covid, so a positive test would not prove Covid exposure/immunity.
Gracias, thank you great information.
Heard the lateral flow test was more faulty than PCR test.
@ Jahill the lateral assay flow test for nasal swabbing states it is 90% effective.
Nice work thanks for sharing