Agrobacterium Mediated Gene Transfer - Part 4 | DNA Cloning

[@BaaYo]

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[@gayatripise4666]

Aap jaise teacher ho to... To hum jaise student ko koe tension nh hota.... Thank you mam ❤

[@shilpadash2899]

You are best teacher mam

[@djrkrlr6003]

Nice teaching Mam,
Just saw your video 20 mins before exam now confident enough to answer any questions on this topic
Thank You Mam

[@shwetadas8201]

Loved your easy explanations. Just a suggestion: please try to incorporate all the information in one video so that it becomes handy and also all the material is found in one place. Thank you so much. Loved your way of teaching. My next go to channel after shomu's biology ❤️

[@Studentkaksh]

Love your videos mam,
You have depth knowledge and you are beautiful 😊

[@BaaYo]

Thank you

[@shanzaymalik864]

It`s so helpful lecture for me.....i never understood this much clearly before today.....Thankyou v. much ma`am

[@014-venkateswarsunani8]

Really ur teaching is so so amazing its brings to clear so many confusion from related topic upload more videos about gene cloning thanks alot mam

[@satyajitdas1370]

It's really been helpful to understand the concept

[@delightful4855]

Weldone di continue your journey, very helpful this type video

[@nundah33]

thank you very much and regard from Mauritius Very helpful to me.Do you have such kind of explanation on plant tissue culture

[@bidishakumari7095]

Wonderful explaination

[@sanariaz2809]

Such a wonderful lecture
Etna acha explain
You are the best 😘
Thank you Soo much ❤️
May Allah Pak bless you

[@sahildasgupta3006]

Thank you so much maam for this series

[@noorkhan6486]

Very informative 👌👌

[@simrannida1608]

Thanku soo much ma'am ❤

[@anubhabpattnayak334]

Thank you for your time and help

[@vrindamiglani1999]

Well explained ❤️

[@SMS.WAHEED]

Mam what about opine sequence yu not discuss the cut of this with axin and cytokine?

[@pushpamandal9948]

Please make a video on genomic library and cdna library

[@priyanshigupta918]

Thanks a lot maam keep up the good work 😊😊

[@toshikmuneshwar4921]

Ty mam very nice explanation
Mam bsc botany ke topic pr our videos banaye

[@ravendrakushwaha]

Very helpful 👍

[@vijayasrivastava1928]

Very Easily explained....👏 Thnk u🤗

[@bhabishyaadhikari7704]

THANKS for this session. I really need you to answer this - When recombinant Ti plasmid is formed after the insertion of gene of interest then by which process it gets inside the plant cell? please reply I am so confused. All other parts I understand completely.

[@nishasinghtomar7818]

Excelled!! God bless You.

[@takini4918]

Thank you ma'am🙏

[@Gaana_madhu]

very well explained thankyou so much

[@mehwishsulman2429]

She clear and understandable.

[@roshan71920]

please spell cytokinin instead of cytokine...i too earlier many times was confused....but well explained....

[@tamanaah-u4g]

Ma'am gene of interest thik se ligate hua to plasmid me ya nahi ye kaise pata chalega?

[@dip7976]

Tysm mam I can understand this topic easily

[@Sciencemind-z2y]

Kadak mam aap bhi or concept bhi 😂

[@mukeshacharya7994]

thank you Mam very nice explanations 😇🤗🙏

[@KIRNCAUR]

Thanks mam ..for this video...
Ur video is very helpful...👍

[@mukeshkorram3697]

Awesome lecture mem.. Pls continue

[@sandilyaraaj1657]

THANKYU MAM VERY NICE VIDEO AND 100% CLEAR

[@Mr.Abd0001]

Thanks

[@nisarahmed5346]

Thanks so much madam

[@rahularora7956]

Very very very helpful

[@cgv9918]

Thankyou very much mam..

[@AmritKaur-cz4yz]

Thank you mam❤❤

[@jatinrana7974]

Mam factors konse hote h isme Jo transfer ko affect krte h
Please btaiye

[@dessythomas7107]

What about the opine synthesis gene? Is that cleaved as well before adding a gene of interest?

[@arundhatianand2658]

Totally understandable..thank you

[@biotechnologist2190]

Mam hame Kaisa maloom Hoga k hamara Jo gene of interest ha wo transfer howa ha ya nahi ??

[@mukeshkorram3697]

Thanks for this helpfull video

[@mandirachoudhury4515]

Thank you so much for the helpful video💌

[@01amitkumarsingh30]

Valuable information
Thanku mam 😎

[@mohammedmateem7697]

Thnku so much ma'am 🤗🤗

[@BaaYo]

Thank you 😊

[@sukanya1413]

Thank you so much 💕🥰

[@innovativeagrisolutions2416]

Can you please tell me uss plasmid ko bacteria k through bhi transfer kr skte hai na?.......
Aapne btaaya auxin aur cytokinin genes ko remove kr dia. Opine gene ka kya hua......?... Wo hmaare recombinant plasmid me tha ya nai?

[@innovativeagrisolutions2416]

Agar artificially transfer kr rahe hai to hmee opine gene ki zaroorat nai hai....
BT agar bacteria k through kr rahe hai to kya hme opine gene ki zaroorat hai?

[@neerajacw]

Superb blog

[@spathshala1988]

Mam, wht about opine genes??

[@SMS.WAHEED]

Mam instead of use of multiple cloning site inserted can we insert direct our target gene sequence.?

[@bidishakumari7095]

I think in that way it will be unable to replicate

[@ravinderbisht487]

THANKS #MAM#

[@ArtfulAura601]

Mam crown gall disease na ho isliye hum ti plasmid istemaal ker rahein hain😦

[@pritykonwar120]

Can I know the direct method of gene transfer please

[@nehasainichauhan3964]

Thankyou mam

[@learnwithme5456]

Thanks mam😊

[@safeerch9346]

mam jab plant wound k bad phenolics release krty hain tu wo kesy Agrobacterium cell mn enter hoty hain,jb phenolics enter hun gye usky baad e vir A gene activate hota hy na,to phenolics enter kesy hoty hain?

[@BaaYo]

signalling se enter hote hai..jaise animation me show kiya gya hai

[@manisapradhan6390]

Thank u

[@BaaYo]

Thank you Manisa

[@shanzaymalik864]

I`m from Punjab, Pakistan....Ma`am I want some helpful lectures from you on more topics....How can I????

[@ankithanayak225]

Which gene of interest

[@akanshagupta4686]

Thankuu 😇

[@nanashendge9284]

Mam 2 number ke video ka sound link ho raha hai

[@immunoglobi]

I am not a science student but still watched full video twice.

[@jineshkumawat7877]

mam i want Autacoids , Hitamine video lecture plzz help

[@mahimatomer8408]

🙏🙏

[@shristiroy5901]

Why we use restriction enzyme??

[@bhabishyaadhikari7704]

It is used for the breakdown of genes

[@garimarai7394]

Plant me disease cause krne s bacteria ko kya benefit h wo esa kyu krti h ?

[@bidishakumari7095]

Ye to bacteria hi Jane uski marzi😂😂

[@mauli1882]

Mam opine ka kya work hota hai??

[@pratikshapandey8972]

Opine gene bhi transcribe hoga plants me jane ke bad but wo plant ke kisi kam ka nhi, wo bacteria ka C and N source hota hai

[@VijayshankerSingh]

You are a biology teacher then why you made video on mass spectroscopy?
BTW I am nuclear physics research scholar and I reached your channel by watching mass spectroscopy video.
I subscribed this channel because I always wanted to learn genetics.

[@ramsaran389]

Ma'am vir gene toh transfer hua nhi sirf T dna hi transfer hua toh vir gene ka expression kaise hoga

[@BaaYo]

Bacteria me hi hoga..

[@BaaYo]

Protein bacteria me hi banegi.. and kam kregi

[@lajpatbhardwaj8356]

m to mam aapka gene cloning vala board as its likh aaya exam me

[@alokkumarsrivastava2959]

Which unit this topic given

[@BaaYo]

Plant biotech...csir syllabus unit 7 i think

[@alokkumarsrivastava2959]

@@BaaYo thnkuu mam

[@Meetkalotra]

Ma'am 12th agriculture biology ke 2nd chapter notes 🥲
Pleaseseseseeesesesese

[@Mayakihara]

Why is your title in English if you weren’t going to speak one word of it?

[@suryakantgaikwad8273]

Thanks