Isoelectric Focusing: A Simple Way to Enhance Your Protein Separation

#112 - Isoelectric focusing (IEF) is a powerful technique distinct from the more familiar SDS-PAGE, [1 ( bitesizebio.co... ),2] ( bitesizebio.co... ) tailored for separating proteins or peptides based on their isoelectric points (pI). [3] ( bitesizebio.co... )
This method capitalizes on the migration of charged molecules through a stable pH gradient until they reach a zone where their net charge is zero, halting their movement.
Essential to setting up an IEF experiment are the IPG strips, equipped with a pH-responsive gel, and the sample, often mixed with carrier ampholytes for efficient migration.
IEF's utility spans various applications, from enhancing 2D-PAGE protein separations to analyzing post-translational modifications and preparing samples for mass spectrometry. [4] ( bitesizebio.co... )
Discover more about how this technique can advance your research and streamline your experimental workflows.
Resources:
1. How SDS-PAGE Works. Available at: bitesizebio.co... ( bitesizebio.co... )
2. Isoelectric Focusing: A Simple Way to Enhance Your Protein Separation. Available at: bitesizebio.co... ( bitesizebio.co... )
3. The Definitive Guide to pH, pKa, and pI. Available at: bitesizebio.co... ( bitesizebio.co... )
4. Detecting Post-Translational Modifications. Available at: bitesizebio.co... ( bitesizebio.co... )
Mentors at Your Benchside
Episode 112
August 20, 2024
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