Implementation of a Multiplex Molecular Test for Anaplasma, Babesia and Ehrlichia

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There are tick-borne diseases beyond what causes Lyme disease. Different geographies have differing prevalence’s of tick-borne infections. In the northeastern USA, some of the other infectious agents are Anaplasma phagocytophilum, Babesia species and Ehrlichia species.
This talk will describe our experiences on the processes used to implement a multiplex molecular laboratory developed test (LDT) for the detection of A. phagocytophilum, Babesia spp. and Ehrlichia spp. in a clinical reference laboratory. We will discuss various testing options including assay types and ordering algorithms. Our experiences and lessons learned after implementation will be discussed, including the testing challenges we had related to COVID-19.
Learning objectives:
Describe the various methods available for detection of Anaplasma, Babesia and Ehrlichia
Understand the process of LDT validation
Discuss advantages and limitations of in-house testing