How I analyze RNA Seq data using HISAT2 , SAMTOOLS , STRINGTIE BALLGOWN & R

[@edpalen5295]

keep up the great work coach. I hope you hit the 100K subscribers pretty soon. What you are teaching is pure knowledge.

[@graphiomics]

Undoubtedly, much more effort you have given for this awesome and brilliant work. Thanks and salute coach.

[@haosun6431]

Thanks a lot! The tutorials means a lot to us as a beginner.

[@bioinformaticscoach]

You're very welcome. You should also watch the tutorial on deseq2.

[@sukeshinikhandagale7271]

thank you so much, you have no idea to what extent you have helped. so gateful for this video

[@vardansaroyan7634]

Great job!!! Thanks!!!

[@grace-426]

why there are dots at some places instead of gene names?

[@Agriacadamycp]

during indexing it showing killed ? how to resolve it

[@humerainayat2858]

I am encountering this error conda: command not found

[@burakkahveci4123]

This is a wonderful work. Thanks your efforts :)

[@hassanishag7211]

Excellent work. But the data failed to be downloaded even with wget. Any thoughts?

[@bioinformaticscoach]

You can raise an issue at my github repo. Send the error message as well.

[@hassanishag7211]

@@bioinformaticscoach DONE, Please check and help. Thank you.

[@bioinformaticscoach]

You can raise an issue on the github page.
Alternatively, you can use curl to dowload.

[@rajneeshdadwal]

Hey, i am starting to learn genomic and transcriptomic data can you please suggest me the pc requirements for a budget pc for the same.

[@sandrajose7452]

Thank you so much for this wonderful video!!

[@subway831]

How did you get gene and transcript abundance? I see that you ran a script.

[@dhananjaimp9036]

Your tutorials are very helpful. Could you please do a tutorial on RNAseq analysis using trinity?

[@niveditabhadra]

Excellent tutorial. Thank you!

[@pelumimagret7447]

You said the recommended PC is Linux . Can I also use my Windows laptop ?

[@MM-fj7ym]

Hi, I have a problem. When I will run hisat2, I found
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
LANGUAGE = (unset),
LC_ALL = (unset),
LC_CTYPE = "UTF-8",
LANG = "en_US.UTF-8"
are supported and installed on your system.
perl: warning: Falling back to a fallback locale ("en_US.UTF-8")
How to fix it?

[@sanjaisrao484]

Ballgown or DEseq2 which is the best sir?

[@AtakanUnlu]

Great video. I can't find the extract_splice_sites.py file. Could you help me, please?

[@mutazmohammed9621]

I'm facing the same problem, and if you figure it out, please help; thanks in advance

[@prunsoopful]

Wonderful!! thank you!!

[@santosh6148]

Great! Thanks for your efforts :)

[@joydipbarua3647]

Thanks for these nice tutorials.

[@vazquezbecerramarioignacio1180]

Hi, good afternon, I have a problem with the script sort_mapped_read.sh It says [W::hts_set_opt] Cannot change block size for this format
samtools sort: failed to read header from "mapped/ERR188044.sam" Thank you