6.8 Biotechnology - AP Biology

In this video, I summarize some of the ways that humans use DNA to advance genetic engineering, making possible things like modified crops, DNA fingerprinting, and more. I explain gel electrophoresis, PCR, bacterial transformations, and DNA sequencing.
AP Biology Essential Knowledge - IST-1.P: Explain the use of genetic engineering techniques in analyzing or manipulating DNA.

[@idk-tl1mo]

thank you SO MUCH for these videos! truly a lifesaver (or a grade saver i should say)

[@poseknowsbio]

It’s my pleasure!

[@cheukyansang9223]

9:19 for the taq polymerase, do you inject it into the bacteria? or there’s this protein that’s in the bacteria? (Pcr happens in the bacteria for replication right?

[@jdiwkim]

hii- 7:46 youre telling that they match suspects dna with dna in crime scene; the thing that I didnt understand is arent the restriction enzymes gonna cut the same fragments in everyone- bc u said restriction enzymes cut DNA at limited number of specific locations, so the same restriction enzyme will produce the same lenght of dna fragments as I understand-so how the electrophoresis works?

[@poseknowsbio]

So DNA that has been treated with restriction enzymes is loaded into an agar gel inside what’s called an electrophoresis chamber. The agar gel has little tiny microscopic holes in it that DNA can pass through. An electric current is applied to the chamber so that it repels DNA, which is negatively charged, from one side and attracts it to the other. Smaller pieces of DNA are able to travel further through the microscopic holes than larger pieces of DNA. These large and small pieces of DNA form a type of pattern. You can tell which pieces of DNA are smaller and which are larger based on how far they travel through the gel. If two DNA samples are the same, they will show the same pattern in the gel if they are treated the same restriction enzymes. That’s how you can tell if two DNA samples match!

[@Mango-sp9om]

i love you so much

[@pieceaisa5046]

i love you man

[@CML3327]

Is it possible to not cover up the info in your slides with your face?

[@poseknowsbio]

I mean I try not to… 😅

[@Noamtiomkin]

@@poseknowsbioDon’t worry for the majority of people it makes it easier learning from a real person you can see! Thanks your videos are great!