How to calculate LOD and LOQ by different ways
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- เผยแพร่เมื่อ 11 มิ.ย. 2019
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How to calculate LOD and LOQ by different ways?
• 6-How to calculate LOD...
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Thank you. May Allah give barakah in your knowledge.
Thank you so much
This video is great, it helped me a lot. Thanks for that!
You earned my subscription even for just the first explanation of the difference between LoD and LoQ, very nice and clear declaration!
It’s my pleasure brother thank you so much for your concern
Salam Mr. KhabeerThank you very much, very well explained, keep it upGod bless you
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Nicely explained, ca u give more examples for related substance impurities LOD LOQ calcutaion. like in one drug product three impurities present, then how to calculate
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Thank you for making this video. Is Limit of quantification and quantitation is the same? What is MDL (Method detection limit), surrogate standards, internal standards, and mixed standards? Can you please address these questions?
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Hello. What do you think about ICH recommendation about calculating LOD and LOQ from the equation of regression based on fortified samples?
Thank you so much for your comment, can you please give me example about your query?
@@AccreditedLaboratory there is a sheet database.ich.org/sites/default/files/Q2_R1__Guideline.pdf and there is some docs www.cysonline.org/article.asp?issn=2229-5186;year=2011;volume=2;issue=1;spage=21;epage=25;aulast=Shrivastava and some examples: www.uttv.ee/naita?id=24440
@@AccreditedLaboratory SO, the main idea is that if I want to calculate LOD or LOQ for METHOD (its important) I should make fortified samples on 5 different concentrations (lowest as possible) with some replications. And then I should make an anlytical procedure with that samples. After that I will obtain results like dependency of signal from concentration (recovery should be over 70% for all samples). Then we calculating regression function and defining the linear range. The error of our regression function will be the complex error of the method. And then we calculating LOD like 3.3 times error of regression equasion devided on slope of function. LOQ - the same but 10 times. The physical sence of it that our LOD and LOQ ever would be grater than any error of method in linear (or close to that) range with recovery over 70% as it should be.
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Could you please explain the effect of standard curve on high quality control and low quality control
Do you mean if the calibration curve is not accepted or slope of the curve not within the acceptance limit
hello, maybe you can help me with the name of some book or paper where they use the third method (use of degrees of freedom) .. it would be of great help
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Well explained sir Can we use this method in raman spectra as Iam facing a problem in LOD and LOQ in raman?
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This can be applied for the method itself with any instrument
Sir.. Is it correct to have limit of detection value higher than the experimentally done minimum concentration in Stern volmer plot?? I am getting like that. How can I correct that?
Can you please clarify the question
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If you are injecting 2 ppb conc. for both LOD and LOQ then how you determine which one is LOD and LOQ?
Can you please care to assist to rectify this confusion?
Thank you.
That’s why I said to select LOQ and from that you will calculate standard deviation that will be used to calculate LOD from 3*S
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What is the guidline or referance that say that lod equal blank+3SD
and that Sd calculated from recovery of expected LoQ
In all guidelines you can find how to calculate LOD AND LOQ separated but you should understand how to apply in the real validation because in each you should select the lowest concentration. So, better to select the lowest conc can be quantified accurately by your instrument and method which is LOQ, then to get the S from that and apply it to LOD equation
Can you please recommend me a good training provider for HPLC, I want to have deep HPLC training in Germany!
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Keep it up
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I think that recovery range is from 98%-102%,
How did you get this limit (70-120%)?
This range depend on the guideline used or as listed in the method or depend on the matrix. This range in Eurachem guidlines and in most of food residue analysis methods
I think you made a mistake at 20:42 the first x is the indivual result and the second x is de average. Other than this the video is very informative thank you.
Thank you so much, I will check that and go back to you inshaalah
@@AccreditedLaboratory habibi no problem 🙂
Shadi Shd just give me the time exactly if you please
@@AccreditedLaboratory 6:07
Yes you are right
ايه المقصود بال maximum residual limit?
Rejection limit. If you get a result which is higher than this limit. So, sample will be rejected
Can you tell the exact reference for lod calculation using replicates
In all guidelines but I said in the beginning I use EURACHEM
In the third approach, how L.O.Q is 0.13, and you said firstly it is 0.2ppb?
Yes it’s 0.2 not 0.13
@@AccreditedLaboratory what is the limits of LOD N LOQ
You did a mistake at the 7 minute in the video...
LOD=mean blank+3Sd of the blank(that's the right )
You said LOD=blank +3SD of the 6 replicate of the lowest concentration detected (that's wrong)
Thank you for your interest. In validation you need to calculate standard deviation for all replicates then you will calculate LOD using this SD by this equation. So it should be replicated if blank sample spiked with known concentration not only blank or how you will get SD
What do you mean by 3SD? you mean multiple 3 with SD? or add 3 different SD? Its confusing
Multiple
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