Fit an AlphaFold database model to a cryoEM map

We show how to start building an atomic model into a cryoEM map using an atomic structure from the AlphaFold database. We fit the atomic model into the map and adjust the position of one of its domains. The map is EMDB 30495 and is of a human dimer originally thought to be an mechano-sensitive ion channel, although the Nature publication speculates it is instead involved in lipid metabolism.

[@Ap31659]

Thnak you so much for this informative tutorial.

[@user-gt9mj9os7r]

Thank you so much for this nice tutorial

[@mamado6625]

Nice tutorial!

[@murpholinox]

Very nice!

[@michaeljcharette]

How would you then add the 2nd copy of the alphafold protein in the cryoEM dimer? Simply repeat the steps, but for the other monomer?

[@ucsfchimerax8387]

Yes repeat the steps to fit the second monomer in the cryoEM map. Or you could instead run a prediction of the dimer by pasting both sequences into the ChimeraX AlphaFold panel separated by a comma. The prediction can take about 30 minutes.

[@michaeljcharette]

@@ucsfchimerax8387Thank you, just seeing this now!