Lab Technician | BSC MLT | phlebotomy | Interview Questions and Answers | in Hindi | Part-2

Lab Technician | BSC MLT | phlebotomy | Interview Questions and Answers | in Hindi | Part-2
Part 1 : • Lab Technician | BSC M...
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Q26. Tourniquets should not be bound for more than ?
Ans. 1 min
Q27. How we prepare chocolate agar
Ans. By heating the blood slowly at 80°C
Q28. How do you prevent hemolysis of a blood sample?
Ans. To prevent hemolysis (which can interfere with many tests):
● Mix tubes with anticoagulant additives gently 5-10 times
● Avoid drawing blood from a hematoma
● Avoid drawing the plunger back too forcefully, if using a needle and syringe, or too small a needle, and avoid frothing of the sample
● Make sure the venipuncture site is dry
● Avoid a probing, traumatic venipuncture
● Avoid prolonged tourniquet application or fist clenching.
Q29. Ideal “room temperature”
Ans. 20 to 25 °C
Q30. Glycated hemoglobin sample is taken in which vial / Vacutainer
Ans. EDTA
Q31. What are the common reasons for canceling a lab test?
Ans. A test that has been ordered may be canceled due to problems unrelated to drawing the specimen, and these are the most common causes for cancellations:
● Duplicate test request
● Incorrect test ordered
● Test no longer needed
A test may be canceled due to a technical problem in the specimen collection process:
● Hemolysis of the specimen
● Clotted specimen
● Quantity of specimen not sufficient
● Collection of specimen in incorrect tube
● Contaminated specimen
● Identification of the specimen is suspect
Delay in transport - specimen too old
Q32. Vial/ Vacutainer used for ESR
Ans. Black (Citrate)
Q33. Why we do not used 100% alcohol
Ans. Because it denature protein
Q34. Difference b/ w plasma and serum
Ans. Plasma is a liquid component of anticoagulated blood whereas serum is a liquid component of coagulated blood.
Q35. Agar is extracted from ?
Ans. Seaweed
Q36. Rt pcr full form ?
Ans. Reverse transcription polymerase chain reaction
Q37. Corona RT pcr sample taken from?
Ans. Nasal & oral swab
Q38. How to prevent hematoma
Ans.
➢ Puncture only the uppermost wall of the vein
➢ Remove the tourniquet before removing the needle
➢ Use the major superficial veins
➢ Make sure the needle fully penetrates the uppermost wall of the vein. (Partial penetration may allow blood to leak into the soft tissue surrounding the vein by way of the needle bevel)
➢ Apply pressure to the venipuncture site
Q39. Difference between vein and artery
Ans. Artery carries oxygenated blood whereas vein carries deoxygenated blood.
Q40. Instrument used to cut tissue samples.
Ans. Microtome
Q41. Universal donor is?
Ans. O negative
Q42. Universal recipient is?
Ans. AB Positive
Q43. Name one Urine preservative ?
Ans. Boric acid, hydrochloric acid, acetic acid, and oxalic acid.
Q44. Which type of urine sample is required for urine culture and sensitivity?
Ans. Mid stream
Q45. Leishman stain procedure
Ans.
1. Prepare blood film.
2. Air dry the film.
3. Cover the film with Leishman's Stain and allow it to act for 1 minute.
4. Add double the volume of distilled water to the slide and mix.
5. Allow the diluted stain to act for 10-12 minutes.
6. Wash the film with distilled water or phosphate buffer of pH 7.0, drain and dry in air and examine
Q46. Natural anticoagulant
Ans. Heparin
Q47. Slide size
Ans. A standard microscope slide measures about 75 mm by 25 mm and is about 1 mm thick
Q48. Sample preservation temperature
Ans. 2-8 °C
Q49. PT sample taken in which vial?
Ans. Light blue
Q50. CRP full form
Ans. C-Reactive protein.
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