How To: Western Blot

In this method video, Nico takes us into the lab to teach us how to perform a Western blot in order to visualize proteins using antibodies.
View this video (and more like it) on LabXchange: www.labxchange.org/library/it...

[@lumpyspaceprincess6335]

I love these videos

[@md.1276]

just looking at it, seems like lots of improvement can be done here. at least shake or invert the blocking buffer few more times so it can mix well, or you might have instant binding of some antibodies and result in uneven imaging signal.

[@Heleous281]

Why tbst vs pbs wash? Absolutely love these lab technique videos!

[@MichaelSmith-ij2ut]

Oh no! Your sleeve was dunking the buffer