Before watching this lecture it was very confusing but now it becomes very easy........thankyou for this lecture .......you are the best.......teacher of biology I have seen ever.......
Thank u so much sir ye video bhut hi helpful hota hai mere liye jb bhi ncert pdhti hu or pura ek unit me nhi kisi usme kisi ek topic me problem aata hai to jldi se 10 min wala aapka ye video dkh kar samajh leti hu.
Sir Aaj ye topic class m kraya ..but Smjh nhi Aaya tha... After taking doubts also... But now it is clear... Seriously ye topic itna easy tha sir..thankuu so much 🤗😇
🤗if anyone dosn't know about chromogenic substrate....so Chromogenic substrate means usually a colourless chemical, that enzymes can convert into a deeply coloured chemical. We use them, to measure if an enzyme is present. Or how much is present. We do that by adding the substrate,then allowing the enzyme to react. Then, we measure how much of the coloured reaction product, was formed.we do that by using a colourimeter or spectrometer, to measure how deep or how strong, the colour is. Chromogenic substrate means usually a colourless chemical, that enzymes can convert into a deeply coloured chemical.We use them, to measure if an enzyme is present. Or how much is present.We do that by adding the substrate,then allowing the enzyme to react. Then, we measure how much of the coloured reaction product, was formed.we do that by using a colourimeter or spectrometer, to measure how deep or how strong, the colour is.
Tq soo much sir I have been struggling more than an hour to understand the example given in cloaning sites but u made me understand in 10 min Tq very much sir
Sir u really amazing...🙏🙏 just keep teaching like this...ek advice de toh sir aap please allen jaise institute jao...waha aapka pakage crore mai hoga...thank u sooo much sir aapke karan mera bio ka exam accha gya..
Sir ncert me diya hua h ki jo ampicillin containing medium me grow krenge or jo tetracycline me nhi krenge wo recombinants h or jo dono medium me grow krenge wo jo-recombinants h or hum recombinant ko select krenge kyu? Or transformants or recombinants me kya difference h? Plz sir solve my doubt
Look, Transformants k two types h : a) Recombinants b) Non - Recombinants Ampicillin medium se hum logo ne Non- Transformants ko remove kr diya... Ab hamare paas bache sirf Transformants (i.e., Recombinants and Non- Recombinants)... Ab haare kaam k sirf Recombinants waale h... Toh ab hume non recombinants ko remove krna h.. Ab apan log pahle ek plate m ampicillin lenge and doosri plate m tetracycline lenge aur dono ( recombinants as well as non- recombinants) ko pahle ampicillin medium m grow kraaenge .. Fir Tetracycline m grow kraaenge... Ab ampicillin m toh dono zinda rahenge.... But tetracycline m sirf non-recombinant( jo ki hamare use k nhi h) waale zinda rahenge ...... ""Ab apan log waapas se ampicillin waali plate se unko nikaal lenge jo tetracycline m zinda nhi rah paae the (i.e., recombinants)".... I hope that I made it very clear to you in simple desi language... 😅😅😅
Sir agr ham r DNA ko tet. Resistant gene pr grow kraenge to vo survive nhi kr paega or uski death ho jaegi . Aise to ham apne desired gene ko further clone hi ni kr paenge. To fir usko plating method se diff. Krne ka faida hi kya hua jb vo dead ho jaega to?? Plz sir ans fast.🙏
Thanking u sir........🙏🙏🙏. Had gone through many videos and didnt understand this part of cloning sites. But now its crystal clear because of your explanation
4:50 VECTORS help in selection of the recombinants and non-recombinants 🙄 ,but we use ELECTROPHORESIS for the selection ???Please! Do reply if u know ..🙃🌸
Okay.,, VECTORS have SELECTABLE MARKERS which help in selection of transformants and non-transformants . But ELECTROPHORESIS is used to separate the DNA fragments on the basis of their sizes. 😅😅
😅😅 they do not die 😂 the TRANSFORMANTS have the Antibiotic Resistance Genes so they grow in the medium while the non transformants do not. So they can be separated easily
RECOMBINANT DNA is the PLASMID DNA carrying the FOREIGN DNA(which we want to use ..). While the TRANSFORMANTS are the host cells after the recombinant is introduced in it. The cells which do not take up the recombinant are NON-TRANSFORMANTS. 🙃🌸🌸
Sir recombinants will die in amp antibiotic nd non rcmb survive....but we need recombinants for process as they died.so we will get no benfit...ans plz sir
Cloning site / Recognition site is the particular/specific base sequence which an enzyme recognises and act on(cut) .also called palindromic sites . Every RESTRICTION ENDONUCLEASE enzyme has its own specific pallindromic site . For exa, 5' GAATTC 3' 3' CTTAAG 5' Its a cloning/Recognition site .🙃🌸
Sir selectable markers and clonning site mein kya diff hai ? If we insert a piece of dna in tet resistance site of pbr322 then what will be the selectable marker ?
@@sangeetadevi5819 we are removing one of the antibiotic gene.... And adding another desired dna segment to rDNA.... Toh jis antibiotic resistant ko hataya... Ussi antibiotic me jb plasmid (rDNA) wala dala... Toh mar hi jaayega na... Qki uski jagha hi hamne naya sequence lagaya h
The plasmid dna in which the desired gene is inserted is known as recombinant dna. The bacterial cell that contains the recombinant dna is known as transformant.
Transformants contain the antibiotic resistance genes so they do grow in the medium containing that particular antibiotics(e.g, ampicilin ,tetracycline ..) while the non transformants do not grow .🙃🙄
sir apne ye jo topic padhaya hai ye CBSE ke syllabus me hai but humare yaha school me teachers ne is topic ko discuss hi ni kiya.sir hum log un par andhavishvas kar ke padh re hai lekin mai bad me sare topics apke channel par padhti hu
VECTOR and GENES are obviously different things . VECTOR DNA /CLONING VECTORS the DNA molecules which carry the foreign DNA segment & replicate inside the host cell. & CLONING GENE 🙄 ??
Mtlb asi genes jinke karan us particukar antibiotic ka.koi asar nahi hota..or isikara hamara bacteria jiske andr plasmid hota hai vo respestive antibiotic me survive kr jata hai
It means that these r a particular gene which are resistant towards antibiotics.eg - PBR322 contain two antibiotics resistant gene such as tetracycline and ampicillin.
bhai clones are morphologically as well as genetically similiar?????? at 12:48 u said transfprmats will grow and non transformants will not grow bhai how is possible because if switch off ampicillin resistance gene it w ill not grow in ampicillin medium and it is a transformant and not growing please tell this how?????please anyone clear my doubt
But if we are introducing alien dna in plasmid at tetracycline resistant gene and it is our transformant then what is the use of selecting non transformants and transformants bcoz transformant will get killed after introducing in tetracycline antibiotic (as its resistance gene has been inactivated). So then we will only be left with the non transformants. Whats the use?!!!!😵😵😐
![ปฏิเสธไม่ไหว (Crush on you) - LIPTA feat. No One Else [TEASER MV]](http://i.ytimg.com/vi/VC689EgdSIw/mqdefault.jpg)
Thnx sir...I was not able to understand NCERT words...but after listening to your lecture I'm able to understand..thnx it's very helpful
Same here dude... he is owsm😊
u have commented two years ago.Then what are u doing now are u selected in neet or else in an medical college
Before watching this lecture it was very confusing but now it becomes very easy........thankyou for this lecture .......you are the best.......teacher of biology I have seen ever.......
Thank u so much sir ye video bhut hi helpful hota hai mere liye jb bhi ncert pdhti hu or pura ek unit me nhi kisi usme kisi ek topic me problem aata hai to jldi se 10 min wala aapka ye video dkh kar samajh leti hu.
thanks sir ....kaise manage kar lete honge aap ki study and hamare liye videos ....really u r great vipin sir
Sir Aaj ye topic class m kraya ..but Smjh nhi Aaya tha... After taking doubts also... But now it is clear... Seriously ye topic itna easy tha sir..thankuu so much 🤗😇
🤗if anyone dosn't know about chromogenic substrate....so Chromogenic substrate means usually a colourless chemical, that enzymes can convert into a deeply coloured chemical.
We use them, to measure if an enzyme is present. Or how much is present.
We do that by adding the substrate,then allowing the enzyme to react. Then, we measure how much of the coloured reaction product, was formed.we do that by using a colourimeter or spectrometer, to measure how deep or how strong, the colour is.
Chromogenic substrate means usually a colourless chemical, that enzymes can convert into a deeply coloured chemical.We use them, to measure if an enzyme is present. Or how much is present.We do that by adding the substrate,then allowing the enzyme to react. Then, we measure how much of the coloured reaction product, was formed.we do that by using a colourimeter or spectrometer, to measure how deep or how strong, the colour is.
Thank u for jabarjsti ka gyan
Thnku
Can you solve my doubt?
But in ncert it is written that E coli doesnot carry resistance against the antibodies you named
Tq soo much sir
I have been struggling more than an hour to understand the example given in cloaning sites but u made me understand in 10 min
Tq very much sir
Sir u really amazing...🙏🙏 just keep teaching like this...ek advice de toh sir aap please allen jaise institute jao...waha aapka pakage crore mai hoga...thank u sooo much sir aapke karan mera bio ka exam accha gya..
Sir up kaise itne easily samjha dete hai.up really too good sir.u r the best👌👌👌👌👌
Thanks sir. Your teaching is awesome. U clear all concepts. Wonderful teaching 😊😊👌👌👌👌👍👍👍
Thanks a lot sir... I was really helpful in clearing all my doubts.
Finally found the video , for which I was looking from past 2 days to clear the concept , thank you so much. ❤️
God he made me fall in love with bio . I hope this relationship with bio lasts long .😂😂😁😁😁
Plz🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏....I request u Vipin sir...,that plz in the last give us time to take screenshots what u had written on board
Sir your videos are really very very simple and helpful..thank u sir
Everything is explained in its finest form thanks sir 👍🏻
Sir ncert me diya hua h ki jo ampicillin containing medium me grow krenge or jo tetracycline me nhi krenge wo recombinants h or jo dono medium me grow krenge wo jo-recombinants h or hum recombinant ko select krenge kyu? Or transformants or recombinants me kya difference h? Plz sir solve my doubt
Look, Transformants k two types h :
a) Recombinants
b) Non - Recombinants
Ampicillin medium se hum logo ne Non- Transformants ko remove kr diya...
Ab hamare paas bache sirf Transformants (i.e., Recombinants and Non- Recombinants)...
Ab haare kaam k sirf Recombinants waale h...
Toh ab hume non recombinants ko remove krna h..
Ab apan log pahle ek plate m ampicillin lenge and doosri plate m tetracycline lenge aur dono ( recombinants as well as non- recombinants) ko pahle ampicillin medium m grow kraaenge ..
Fir Tetracycline m grow kraaenge...
Ab ampicillin m toh dono zinda rahenge....
But tetracycline m sirf non-recombinant( jo ki hamare use k nhi h) waale zinda rahenge ......
""Ab apan log waapas se ampicillin waali plate se unko nikaal lenge jo tetracycline m zinda nhi rah paae the (i.e., recombinants)"....
I hope that I made it very clear to you in simple desi language... 😅😅😅
@@naveenvarshney5587 best explanation
@@naveenvarshney5587 ni aaya samjh
@@anushkatripathi774 dear sister, ab aapko koi nhi samjha skta....
Aap yaad krlo isse😂😂
Brilliant explaination....thanks a lot for clearance of my confusion ☺️
Thanks bro......
Really lt's soooo helpful to read ncert💖💖💖💖
@@123facts3 ha ye ladki sab jagah aa jati h
Haa Bhai meine bhi dekha hei🤣🤣🤣
Hey u
Sir agr ham r DNA ko tet. Resistant gene pr grow kraenge to vo survive nhi kr paega or uski death ho jaegi . Aise to ham apne desired gene ko further clone hi ni kr paenge. To fir usko plating method se diff. Krne ka faida hi kya hua jb vo dead ho jaega to??
Plz sir ans fast.🙏
Yah... Evn i have the same question...
Thanking u sir........🙏🙏🙏. Had gone through many videos and didnt understand this part of cloning sites. But now its crystal clear because of your explanation
sir agar altered wale mar hi jayege to benefit kya hua phir????
@Vijay.kumar Vk yup although i got the answer why so?
Antibiotic resistance Gene ko jab hum foreign Gene se replace karenge to hum bacteria ko antibiotic se Mar payenge.
The altered ones don't die..they just don't grow .
Amazing video 👌 Thanks sir . You cleared all my doubts 😃
4:50 VECTORS help in selection of the recombinants and non-recombinants 🙄 ,but we use ELECTROPHORESIS for the selection ???Please! Do reply if u know ..🙃🌸
Okay.,, VECTORS have SELECTABLE MARKERS which help in selection of transformants and non-transformants .
But ELECTROPHORESIS is used to separate the DNA fragments on the basis of their sizes.
😅😅
What is use if they die in tetracycline medium and we do not have anything useful product?
😅😅 they do not die 😂 the TRANSFORMANTS have the Antibiotic Resistance Genes so they grow in the medium while the non transformants do not. So they can be separated easily
SIR U R A GREAT TEACHER OF BIOLOGY OF RECENT TIME
Sir what is the difference between tranformants and recombinants?
RECOMBINANT DNA is the PLASMID DNA carrying the FOREIGN DNA(which we want to use ..).
While the TRANSFORMANTS are the host cells after the recombinant is introduced in it.
The cells which do not take up the recombinant are NON-TRANSFORMANTS. 🙃🌸🌸
@@sangeetadevi5819 thank you so much😊🌹
Thank you so much sir I really appreciate your hard work thank you for clearing my concept ❤️❤️❤️❤️❤️❤️
Selection marker ke baare me thoda example se smjhaye Sir
A SELECTABLE MARKERS is a gene that helps in identifying and eliminating the NON-TRANSFORMANTS . For example ,, the Antibiotic Resistance Genes .
Sir recombinants will die in amp antibiotic nd non rcmb survive....but we need recombinants for process as they died.so we will get no benfit...ans plz sir
Thank you so much bhaiya. All clear
Sir plzz one request for you,
Plzz can you explain cloning sites in simple language by giving some examples ...
Cloning site / Recognition site is the particular/specific base sequence which an enzyme recognises and act on(cut) .also called palindromic sites . Every RESTRICTION ENDONUCLEASE enzyme has its own specific pallindromic site . For exa,
5' GAATTC 3'
3' CTTAAG 5'
Its a cloning/Recognition site .🙃🌸
Gr8 sir
Very simple and easy! To understand
Thanks sir it's very helpful
Sir selectable markers and clonning site mein kya diff hai ? If we insert a piece of dna in tet resistance site of pbr322 then what will be the selectable marker ?
11:45 agr woh survive hi ni karega.... Toh transformation kaise karenge hum... Qki recombinant wala toh mar gaya na antibiotic daalne ke baad
mera b same doubt
Recombinant DNA = VECTOR(e.g, Plasmid) DNA + FOREIGN DNA & it is antibiotic resistant .
So how will it die ??🙄
@@sangeetadevi5819 we are removing one of the antibiotic gene.... And adding another desired dna segment to rDNA.... Toh jis antibiotic resistant ko hataya... Ussi antibiotic me jb plasmid (rDNA) wala dala... Toh mar hi jaayega na... Qki uski jagha hi hamne naya sequence lagaya h
It's not like that ..sis
@@sangeetadevi5819 😥😥that's why m confused
kitni piyari smile rehti hai sir apke face pr😇🙏🙏😁
Wow!!Thanks a lot ..love from kashmir💕
Sir please named the lectures in serial number
It is so helpful 😊 😊.. thanks a lot
ap mujhe ek baar confirmly bata dijiye ki Jo bhi topics ncert me hai wo to CBSE ke syllabus me he hi na
Yes😂
Sir difference in recombinant and transformant kya hai plz reply sir
The plasmid dna in which the desired gene is inserted is known as recombinant dna.
The bacterial cell that contains the recombinant dna is known as transformant.
M not able to increase The quality of the video ..😢 bhottt light sa dikhra h video me proper nhi dikh pa rha
Bhaiya app ki teaching bahut achi hi.keep it up👍
Very helpful thnxx alot sir
Sir you are wrong we should insert Gene which code beta galactosidase not direct enzyme👍
😊😊
before i see your video and after read ncert book then i ful understand sir thanks
Sir 12th ka chapter 1 2nd upload nhi kie h
how can u differentiate transformant and non transformantby selectable markers pls …...answer this!
Transformants contain the antibiotic resistance genes so they do grow in the medium containing that particular antibiotics(e.g, ampicilin ,tetracycline ..) while the non transformants do not grow .🙃🙄
Transformant hote kya h
Please made trick for colour of compounds ,melting points ,boiling points...bro
Osm explaination
sir apne ye jo topic padhaya hai ye CBSE ke syllabus me hai but humare yaha school me teachers ne is topic ko discuss hi ni kiya.sir hum log un par andhavishvas kar ke padh re hai lekin mai bad me sare topics apke channel par padhti hu
Its really very helpful
👌👌👌👌very well explained
sir tissue ka trick digiye na
Is there any difference between cloning vectors and cloning gene?
VECTOR and GENES are obviously different things .
VECTOR DNA /CLONING VECTORS the DNA molecules which carry the foreign DNA segment & replicate inside the host cell.
&
CLONING GENE 🙄 ??
Nice explain
Thank you so very much ... this video was a life saver !
👍👍👍👍👍superb😘😘
Thank you so much sir 😊😊😌
Sir terminology english use karye par thoda hindi me samjhae ye
Superb
You are greater
ur videos r so helpful bless u sir
Thank you so much sir....Doubt ab clear hua
What difference between ori and cloning site
What is transforments?
Is it recombinants
It is not recombinant it is the organism which is being transformed after receiving the recombinant DNA...
It is not recombinant it is the organism which is being transformed after receiving the recombinant DNA....
Thanks bhaiya 😘 😘😘 😘😘 😘
Super vipin sir😘😘😘😘😘😘😘
Sir can you increase your video quality ? ☺☺
Sir Bam H1 is altered is that means Sal 1 is also altered ?
U R AWESOME GREAT1SIR JI NEXT PLANT PHYSIOLOGY..
Osm sir
When you don't touch a chapter because of some sub topic. Just join here and clear your doubts.
Thanqqq sirr super class👌
Vipu bhai 😂😂😂😹😹👍 gajab
Sir trick is also very helpful
Thnks sir☺
Sir you are best
Best Yr best tq
Kindly...explain in english Completely
Sir beta galactocidase enzyme h
give clear explanation about transformants please!!
sir ye wala vedio aapka Nhi samjhme ara h...... sir simple language me batao "antibiotics resistance gene" ka Kya meaning h
U are good at explaining things perfectly😇
Mtlb asi genes jinke karan us particukar antibiotic ka.koi asar nahi hota..or isikara hamara bacteria jiske andr plasmid hota hai vo respestive antibiotic me survive kr jata hai
It means that these r a particular gene which are resistant towards antibiotics.eg - PBR322 contain two antibiotics resistant gene such as tetracycline and ampicillin.
please make video of chapter 9 sir
Thanku soo much
bhai clones are morphologically as well as genetically similiar??????
at 12:48 u said transfprmats will grow and non transformants will not grow bhai how is possible because if switch off ampicillin resistance gene it w ill not grow in ampicillin medium and it is a transformant and not growing please tell this how?????please anyone clear my doubt
sir I didn't get the 2nd point.selectable marker wala.
pls help me
neeti maske
th-cam.com/video/APs2lQEoXoc/w-d-xo.html
thaks a lottttttttttttttt bcz mko yh smj kbi ni aaya tha
thanks alot sir ji
Thank u sir thank u so much
Thanks a lot.
But if we are introducing alien dna in plasmid at tetracycline resistant gene and it is our transformant then what is the use of selecting non transformants and transformants bcoz transformant will get killed after introducing in tetracycline antibiotic (as its resistance gene has been inactivated). So then we will only be left with the non transformants. Whats the use?!!!!😵😵😐
I too have the same question 😝
Tysm sir ❤️
Super bhai😂😂😊
how gene are formation
Thank you bhaiya ..awesome way of teaching