This is great but you don't address how the cells know which strand to repair and your gap (nick) that needs to be closed by DNA ligase is in the wrong place - it should be the 3' side of the newly added nucleotides, not the 5' side (so closer to where PCNA was).
@@hussainbiology I think I understand. During DNA replication the parental strand is methylated. It is then copied. It is assumed that the parental strand is correct. With the mismatch repair proteins any mistake in the daughter strand is repaired. 🧬 ✅ Shabir, Another solid video! Thank you so so much. I don't know if you have the energy to do a deep dive into just one of those proteins and show the active site and how any mineral co-factors are added during their construction. The molecular 🔬stuff is very interesting. 👏👏👏👏👏
@@michaeleisenberg7867 Methylation to recognise parent strand is done in prokaryotes.. In eukaryotes presence of lagging strand (the okazaki fragments ) is used to recognise the new synthesised or daughter strand.
@@anshikamishra1292 Thank you. I would like to know how the metal cation gets placed in the active site of the various polymerase enzymes? Who puts it in there?
I like it the repair mismatch animation please upload the series of DNA repair I hope you give us great knowledge....thanks shabir hussain..
This is great but you don't address how the cells know which strand to repair and your gap (nick) that needs to be closed by DNA ligase is in the wrong place - it should be the 3' side of the newly added nucleotides, not the 5' side (so closer to where PCNA was).
What happens if it's the opposite strand that has the incorrect nucleotide?
Parental strand will never become one if not repaired at first place..
All daughter strands will become parental once repaired after Replication
@@hussainbiology I think I understand. During DNA replication the parental strand is methylated. It is then copied. It is assumed that the parental strand is correct. With the mismatch repair proteins any mistake in the daughter strand is repaired.
🧬 ✅
Shabir, Another solid video! Thank you so so much. I don't know if you have the energy to do a deep dive into just one of those proteins and show the active site and how any mineral co-factors are added during their construction. The molecular 🔬stuff is very interesting.
👏👏👏👏👏
@@michaeleisenberg7867 surely i will do
@@michaeleisenberg7867 Methylation to recognise parent strand is done in prokaryotes.. In eukaryotes presence of lagging strand (the okazaki fragments ) is used to recognise the new synthesised or daughter strand.
@@anshikamishra1292 Thank you. I would like to know how the metal cation gets placed in the active site of the various polymerase enzymes? Who puts it in there?
Mahakal bless you my son keep doing jai shree ram