Paper Chromatography = Separation of Amino Acids Mixture by Paper Chromatography Technique (ENGLISH)

This video is also available in HINDI. Separation & Identification of Amino Acids by Paper Chromatography| Rf value Calculation (HINDI) • Separation & Identific...
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.
Paper chromatography is one of the types of chromatography procedures which runs on a piece of specialized paper. It is a planar chromatography system wherein a cellulose filter paper acts as a stationary phase on which the separation of compounds occurs.
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[@mehtamohit4831]

There is the Correction in Retention Factor (Rf) that unit "cm" is shown in Answer. But Rf has no Unit.
Please Notice That Thing.

[@solutionpharmacy]

Thank you for the rectification...We accept that we have made mistake in it

[@abishekg2828]

Thanks bro

[@kumarvaibhav4937]

Thank you so much to explain this in very easy way.

[@janvibairwa4760]

Thank you for this ❤️

[@nathanitaxak3377]

ThankYou.

[@subhashkalagapudi8211]

Wat a voice........,tqqq so much

[@sambitdas5065]

It's really nice explanation 🙂

[@Simran-jd4wi]

Thank you ❤

[@kollibalakrishna314]

Thank u very much

[@jarulatha5757]

I like this mam very much interesting

[@harshitharajkumar6430]

Well explained maam

[@rashmikumaria848]

Very well explained mam..found it really helpful..!!!

[@solutionpharmacy]

Thank you

[@mahendrans0kannan485]

AmZing..tq

[@piyushsen7502]

Thnqu

[@manims4929]

Tq mam😊

[@akhilatm1561]

Tcq so much

[@tejamatta829]

very useful mam excellent explanation , can u explain total chromatography unit in instrumental analysis in semester -7. it is useful for easy way to understand

[@sangeethak.s1411]

This is real good video

[@solutionpharmacy]

Thank you so much

[@dipmalagurav6592]

Plz help me how to prepare chemical to used chromatography technique

[@jyothikollur6334]

Tqq so much sir it's really effective by looking into pratical then byharding the end colur

[@solutionpharmacy]

Thank you

[@durgeshmishra7094]

Ha jo 20 ko practical ho rha hai ignou ka

[@14pradnya1]

Nicely explained...

[@solutionpharmacy]

Thank you

[@DrSanjeevPandey]

Excellent

[@solutionpharmacy]

Dr. Sanjeev Pandey thank you

[@rajbanjare7902]

Ty for this vedeo

[@solutionpharmacy]

My pleasure

[@palwindersingh1398]

Thank you

[@solutionpharmacy]

My pleasure

[@harishs1762]

Mam for me sample is not moving for long distance what may be the error

[@moniruzzamanmonir4227]

Thanks mam from Bangladesh.

[@solutionpharmacy]

Thank you

[@RajkumarRajkumar-er7nk]

Mam go little bit slow pls.but explanation is good👍

[@sneharana9985]

Well in yt there is an option for playback speed for your comfort

[@shivamnimbalkarofficial6335]

Nice information 👍🙏

[@rajilakshmi5133]

Hi
Can u please clarify regarding How to prepare unknown solution and standard solutions

[@rohithpoojary1992]

Yes maam, I also have the same doubt, plzz can u explain that with another video

[@itsrd3523]

Nice Video

[@solutionpharmacy]

ITS RD Thank you

[@SanguinaryStrife]

What is the solution used in the beaker that saturates the filter paper? You spoke a little to fast, I didn't understand.

[@solutionpharmacy]

Same mobile solution

[@katherinecantwell5539]

So, in the video, was the unknown solution a combination of the two known amino acids? Is there a standard that I can use to determine what amino acid I have if I don't have pure samples to compare? Thank you!

[@abeersiddiqui5670]

unknown solution was a different amino acid and the standard to compare your tests with, their is a standard text or book with different rf values of amino acids in diffrent solvents

[@Pujakumari-qx2zb]

can you plz explain how can we separate amino acid from blood? plz plz plz plz

[@keyllaperez5139]

What are the amino acids in the samples? And what is the purpose of exposing the dipeptide samples to a 6N HCI solution ? Ty

[@bullseye3054]

sample a leucine and sample b is valine. she said but i just realised it after i i kept looking for it

[@bullseye3054]

i know i replied to late but it might help someone else idk

[@vairamuthus7160]

What is the solvent in capillary tube ?

[@bldmontemor]

@@vairamuthus7160 The thing in the capillary tube is the sample/amino acids.

[@ramdhanfadhil9687]

Why we need to dry the paper in oven after spray it with nyhidrin?

[@solutionpharmacy]

So that colour may develop

[@avnish5352]

Mam amino acid ka mixture lo and uske baad seperate krke dikhao.
Mixture ko kese seperate kare,

[@rajamanohar3192]

U dont take Samples in Beakers !

[@yamankumarsahu5757]

Made in hindi video please

[@solutionpharmacy]

yaman kumar sahu Iska hindi me bhi hai aur pura hannel hindi based hai

[@projjalsanyal4509]

This is so wrong, a chromatographic chamber should be used a beaker has dip which means evn when covered it won't br completely closed and thus won't be saturated properly

[@user-yk7mu1ri2m]

واو ماما

[@jannatulferdaus3045]

দ্রাবকে কি কি পদার্থের মিশ্রণ থাকে।

[@syedhafeez3711]

I have two questions
1. Preparation of mobile phase and
2. Sample preparation.
Mobile preparation is not clear , as I can see very few ml of m.p, does your m.p contains water? Or discarded it?
You have dissolved amino acids in distilled water instead of m.p.
Kindly clear this.
Thank you.

[@kranthiarravati6958]

Hlo tharun unava