ADDITIONAL VALIDATION APPROACHES The fusion of eGFP to a protein additionally allows researchers to observe the spatial and temporal localization of that protein inside live cells using fluorescence microscopy. eGFP (enhanced Green Fluorescent Protein) can also be used to track and compare protein-protein interactions in wild-type (WT) and mutant proteins through immunoprecipitation (IP) experiments. Immunoprecipitation allows the isolation of a protein of interest from a cell lysate, often along with its interacting partners. Proteins can also be tagged with small epitope tags such as FLAG, HA, and Myc during expression to facilitate purification, detection, and analysis.
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ADDITIONAL VALIDATION APPROACHES
The fusion of eGFP to a protein additionally allows researchers to observe the spatial and temporal localization of that protein inside live cells using fluorescence microscopy.
eGFP (enhanced Green Fluorescent Protein) can also be used to track and compare protein-protein interactions in wild-type (WT) and mutant proteins through immunoprecipitation (IP) experiments. Immunoprecipitation allows the isolation of a protein of interest from a cell lysate, often along with its interacting partners.
Proteins can also be tagged with small epitope tags such as FLAG, HA, and Myc during expression to facilitate purification, detection, and analysis.