Thanks a lot for this video! It's hard to find a good explanation to the topics NRPS and PKS on the internet. You did a very good job here :) But at 0:46 min I guess you meant "each *MODULE* typically has an A, PCP and C domain" right? Also: - Some sources call A the "Activation domain" and others call it the "Adenylation domain". What's right here? - You named the "TE domain" a transesterification. But at Wikipedia I read that TE stands for thiosesterase. This isn't the same, is it? - You are a quite fast speaker. For people like me, whose native language isn't English, it is very hard to follow you, so I had to click "pause" and scroll back like a hundred times ^^ But all in all you gave me a very good introduction to this topic. Thanks you :)
i am writing my thesis on polymyxins (apparently they're synthesized via this nonribosomal pathway)...Thank you for the video since it really helped me and if you know any not overcomplicated material on this let me know..thanks again
1:05 are you sure is transesterification? looks like amide bond formation. 1:30 are you sure is transesterification? looks like thioesterase activity Thank you for the video
Fair point, I'm using the word transesterification a little loosely. The amino acid starts as the bare acid and is then phosphorylated to an adenylate, which is not strictly speaking an ester in the sense that the carboxyl group is attached to phosphate (PO-) rather than an alcohol (CO-). Often chemists will still call that an ester of sorts. When the amino acid is then transferred to a thiol on the protein, the product is correctly called a thioester, and the term transesterfication is typically used to describe the formation of thioesters as well as regular esters. The next step where the amino acid is transferred to the amino end of the NRP would more accurately be called a transamidation, but it is not uncommon for chemists to call this reaction transesterification. During cleavage of the NRP from the enzyme, different things can happen depending on what type of termination domain is on the NRPS. Some encode thioesterase activity, meaning the attacking group is water and the free acid results. Others do transesterification in the proper sense. Others do transamidation. And there are a few other mechanisms.
This video is helping me pass my Fungal Secondary Metabolism Module thank you!!!
you are saving my degree! Thank you so much!
You're welcome, and I appreciate the comment.
Thanks a lot for this video! It's hard to find a good explanation to the topics NRPS and PKS on the internet. You did a very good job here :)
But at 0:46 min I guess you meant "each *MODULE* typically has an A, PCP and C domain" right?
Also:
- Some sources call A the "Activation domain" and others call it the "Adenylation domain". What's right here?
- You named the "TE domain" a transesterification. But at Wikipedia I read that TE stands for thiosesterase. This isn't the same, is it?
- You are a quite fast speaker. For people like me, whose native language isn't English, it is very hard to follow you, so I had to click "pause" and scroll back like a hundred times ^^
But all in all you gave me a very good introduction to this topic. Thanks you :)
Thank you, short, clear and precise.
I'm writing a paper about a PKS-NRPS hybrid, named Colibactin. Thank you for this usefull video!
God! you just saved my paper! thanks a lot and keep it going! God bless you! God bless you!
Informative and useful.........!!
Can you make more of these? of make them longer? =D thanks!!!
i am writing my thesis on polymyxins (apparently they're synthesized via this nonribosomal pathway)...Thank you for the video since it really helped me and if you know any not overcomplicated material on this let me know..thanks again
1:05 are you sure is transesterification? looks like amide bond formation.
1:30 are you sure is transesterification? looks like thioesterase activity
Thank you for the video
Fair point, I'm using the word transesterification a little loosely. The amino acid starts as the bare acid and is then phosphorylated to an adenylate, which is not strictly speaking an ester in the sense that the carboxyl group is attached to phosphate (PO-) rather than an alcohol (CO-). Often chemists will still call that an ester of sorts. When the amino acid is then transferred to a thiol on the protein, the product is correctly called a thioester, and the term transesterfication is typically used to describe the formation of thioesters as well as regular esters. The next step where the amino acid is transferred to the amino end of the NRP would more accurately be called a transamidation, but it is not uncommon for chemists to call this reaction transesterification. During cleavage of the NRP from the enzyme, different things can happen depending on what type of termination domain is on the NRPS. Some encode thioesterase activity, meaning the attacking group is water and the free acid results. Others do transesterification in the proper sense. Others do transamidation. And there are a few other mechanisms.