White Blood Cells hunt Bacteria under the microscope
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- เผยแพร่เมื่อ 9 เม.ย. 2021
- Phagocytes are White Blood Cells (Leukocytes) that engulf bacteria that have found their way into the body. They are part of cellular immunity. In this video, we can see them move about.
If you want to see them yourself, here are a few tips:
1. They move slowly, so be patient.
2. Close the condenser all the way to get a good contrast and depth of field.
3. Use a small drop of blood only. This way the cells are spread over a larger area and they can be seen more easily.
4. Wait until the blood stopped moving under the microscope slide.
Technical Details:
Contrasting system used: Differential Interference Contrast (DIC)
Magnification: with the exception of the overview clips of the Red Blood Cells at the beginning, all clips were filmed using a 100x oil immersion objective. The total magnification on screen differs, because I cropped the videos.
Camera: Canon EOS 70D, 4K video original footage and then converted to 1080p for TH-cam.
Scale bar: was not included due to different magnifications. The diameter of one Red Blood Cell is 8 microns (8 micrometers). I used mostly 60x and 100x oil objectives. Can also be seen with 40x objectives. [Video 189]
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I want to publicly thank my white bloodcells for working for my health 24/7.
I'm proud of u bros and sisses
yes well done chaps and gals
you deserve a medal
@@LukeFlegg they can't wear that, they'll most likely try to swallow the medal
@@itskittyme true.
@@itskittyme if it's part of you then it's DNA wouldn't be male and female, it'd be only one
@@LukeFlegg😂😂😂😂
Amazing! It's like our bloodstream has its own Roomba that goes around and vacuums up the things that don't belong there! Bacterium!
Lmaoooo
@@Idontgothatway that is how the bloodstream works. I've studied anatomy and physiology- have you? The Roomba is just a fun way to imagine it💝
@@laurabarber6697 I’m laughing because that’s a perfect example….
The sudden plot build mid narration 🤣
@@Idontgothatway Except this Roomba is actually alive
Your sacrifice in the form of your own sweat and blood in the name of the science is appreciated, Oliver :)
It's fascinating to see the white blood cell at 5:18 appear to eat and destroy a diplococcus bacteria! It also appears that another diplococcus bacteria gets stuck to the back of that white blood cell and hitches a ride as the WBC travels around the blood.
Thanks buddy for the time stamp. I also saw it.. It's very fascinating.
i think the bacteria which hitches the ride is a gas bubble, they merged at the same timestamp
@elkstereidolon3523 what
The things moving are bacteria eating debris thus cleaning, not white blood cells. This guy explained by reading bullcrap from wiki
Thank you Mr. Timestamp
Absolutely cool! Thanks MH. What a teacher you are. I'm only a 'greenie' in microscopy, but already fell for anything amoebe-like
I'm trying to grasp blood contents and how they operate for my class. Thank you, I'm a visual person and this really helped!
Congratulations! Your channel is wonderful: your competence and didactics are excellent, your content is very instructive and, in addition, your optical equipment is fantastic. Thanks!
The lamellipodia and filopodia of the neutrophils are incredibly well imaged at the end of the video. Magnificent!!
Thanks for sacrificing some white and red blood cells for our edification.
Your videos never fail to amaze me :)
Fascinating!!!! 😛 Getting to know “You” in a different way! 😝💕
Fascinating! I've learned so much from your channel, thank you so much for taking the time to make all these videos. If my biology teacher had been as enthusiastic and passionate as you are I would have taken it at GCSE, way back in the 80's when I was at school! Much love from the UK ❤
Do you like Doctor Who?
@@gunnerdavidson7287 Yes 😉
Great video, sir!
Excellent video! Thanks a lot!
Nice good job that's awesome I had no idea.keep up the good work 👍
Oliver,
I did not know that you had DIC setup for your microscope. Did you acquire these components
used (?) …..or did you use a microscope at a local University ?
What DIC objectives do you own (brand & magnification) ?
Did you configure your Olympus CH40 for differential interference ?
The live images you obtain with 60x and 100x are incredible !
- - - - - - -
Thanks for all of your amazing, HIGH quality, hard, work.
Thank you so much, great video. What program are you using for video editing to get more magnification? It would be great if you could do a video on this. Thanks.
Were some of these videos in phase contrast? I would love it if you always put the magnification and whether it was phase contrast at the top of the video. This looks great, though. Thanks for sharing.
Thanks youtube for the suggestion.
Thanks uploader for making the video, extremely informative
Awesome video man
¡Muy interesante! This microscope footage of white blood cells is absolutely mesmerizing! It's incredible to see these tiny cells in action, moving around and actively seeking out and engulfing bacteria. Kudos to the creators of this video for making such an informative and engaging piece of content! 🔬🦠🤯
So glad I found this! Phaggy looks like a plainclothes cop scouting out the crowd, looking for the trouble makers.
Beautiful footage of Neutrophils in action! They are fascinating to watch
Great footage
Beautiful vid. thank you!
I liked the format of this video! Did you use differential interference microscopy? What can I do to see phagocytes in a brightlight microscope? Stain of Wright maybe?
This is going to come helpful for my A and P class
Pls show us how to isolate a singular RBC & puncture it so we can see the contents that spill out. Thank you so much for what you do-- amazing channel!!👍👍👍
Fascinating!
Thanks for posting this! I see some tail or tentical structures on the white blood cells, presumably to sense bacteria?
You should add on the description the. brand/model that you are using. That would be really helpful ❤
Would love to see the different immune cells-- leukocytes, neutophils, t cells etc. Thank you!
I can't help thinking about cells at work
Super! I wish we had thoses videos in my histology classes
Whats a good entry level microscope to start capturing and recording live video with good magnification???
Some seriously excellent footage here, nice one.
Coolest thing I’ve seen all year! And the year is just ending
Nice video bro 👏👏
Great video
Amazing!
Very nice video. Is the reason white blood cell counts in auto immune diseases are reduced because as they attack on all cells (not just bacteria) they die off? or is there another reason? Thanks for the vid. - brief but to the point!
This is beautiful thank you.
It's like White Blood Cells from Cells at Work playing Metal Gear and crawling through the vents when they want to squeeze in between cells.
Very cool thank you
Fascinating. The beauty of our systems.
Have you ever seen blood that is mostly yellow under the microscope? My blood is not like any that I've seen. It's more yellows, greens, and purples than reds. I don't remember the last time I got sick. I'm not anemic, cuts seal almost instantly unless it's to the bone or an artery.
hello, nice video - question - on the beggining of video - what magnification is used? 100x? 1000x? 2000x? Thank You.
You are awesome buddy❤️❤️💜
Que lindo! Muito obrigada pelo vídeo
Wow fabulous vid
THANK YOUUU SOOOOOOOOOOOOO MUCH!!!!!!!!!!
Awesome!
2:20 that’s so cool that it’s literally alive like a little animal. Emergence is crazy man, all this little creatures make up us
Thank you so much for your excellent videos. My son is 7 and I just bought a biological microscope. We are very excited to see our own red blood cells.
Blood cells:Why were all outside let us back to body!
dude how cool its to see opsonization..
what do you do as a professional?
Why did that one cell have little spikes on it? Phaggy didn't seem concerned.
thanks to open the world of microscopy to me
Did you ever try to apply differents sound frequency ?
The way the phagocytes move through, over, and under red blood cells brings to mind someone pushing through a crowd going "Excuse me! Pardon me! Comin' through!"
I took Microbiology with the Lab. It was my favorite class.
Fascinating ❤
Fantastic!!!!!!
Nobody noticed that the first sample images has separate red blood cells, and the second, when he explains that a white blood cell is moving, the red ones are aligned together in the same position forming groups. For an unadvertised eye this is nothing, but when you know, then you understand that the second sample has the effect of the exposition to EMF´s (electro magnetic fields) which depolarized them, lacking the magnetic strength to repel each other and keep their phisical frontiers in order to work, and avoiding the clusters, precursors of blood clots. Please give this comment a thumbs up due to the importance of this info. The people must know this.
The stacking up of the red blood cells is called Rouleaux formation and happens everytime when the blood starts to dry up, and when there is sufficient blood beneath the cover glass. In the sample where the blood cells do not stack up, I used very little blood so that the blood cells are limited in their movement beneath the cover glass. This is why they are in parallel to the microscope slide and visible from the top. They are not able to stack up because they can not turn 90 degrees due to the limited space. This has nothing to do with EMF and it was also the same blood sample, just prepared differently.
That was interesting thank you.
Poor lil guy is fighting a war for us and doesn't know he's outside the body :(
0:26 what’s that spiky res blood cell that isn’t moving ? Is it a dead one that’s cell wall broke?
what are the tiny little dots moving around quickly? bacteria?
absolutely fascinating
What are the tiny little fast moving wigglers ?
Amazing
Interesting, I'm keen on repeating this. Dies it have to be my blood or can it also be from one of those people in the basement?
Incredible video!!! The definition allow to see the nucleus and small organelles in the citoplasm. I imagine a neutrophile with a 2 nucleus. I suposse you are using the DIC microscope. I repeat the experiment at home with a normal micro in bright field using the program Topview that comes with a chinese camera and the video from timelapse is beautiful, worth it to try. You feel the cell is alive even if not so detailed. Thanks for you channel.
Yes it is DIC.
Hey Oliver, I am new to microscopy do you think the swift 380b is better than the omax 82es. I’m in Canada. The omax is cheaper but is it worth it?
As long as they have a condenser with a diaphragm, they are the same.
That’s really so amazing
Wow. Amazing.
Fascinating 😊
I love those little guys! 🏥
So cool 😎
@Microbehunter Why do some of the red blood cells look like they are spiny?
Due to loss of water (they dry up)
Great video. What is the cell just on the right of the white cell at 7:10, with the spikes?
Whoaa, you're right the shape os different than all the others.
What type of microscope to see that
Thank you so fascinating the processes that keep humans alive. God is in the details. Watching from Sydney.
“Bacteria can’t escape these predators” Y.Pestis:
pls do another video with of WBC with the blue dye as it is easy to spot them
Amazing definition! Am wondering what those other tiny dot like things are that are moving independently between the voids around the red cells?
Yes me too.
@@adagioforstrings007 gas bubbles?
Yes I was also wondering as I recently saw that similar movement when viewing my own blood but I could see it clearer and looked more parasitic but I still have no idea. Any help would be interesting and welcome 👍🏻✌🏻
What are those stationary "icebergs"? Are those platelets?
How did you take such clear pictures at 1000x magnification? Is it brightfield? Phase contrast?
Also, it would be nice if in your videos you could write these informations in the video itself such as:
1000x mag
20x time speed
Brightfield
Perhaps adding also a scale to be able to roughly measure what we are seeing.
It is Differential interference contrast. Almost all videos were made using a 100x oil immersion objective, but putting a magnification next to it is almost meaningless, because it differs from clip to clip because I zoomed in (I cropped) therefore changing the total magnification on screen. I will add this information in the description.
@@Microbehunter Thank you! The video looks great!
nice sample :·)
did you put heparin on the slide to keep from clotting?
No, nothing added.
Nice! I have yet to catch white blood cells in action. BTW, you will get much more blood per stab if you use the lancet at a corner of your finger
can I see these white blood cells with my new swift stellar pro t? I dont know what I'm doing wrong or do you need a different objective or different microscope. please answer I really want to see this!
You can see them, but need to close down the condenser for maximum contrast. They move slowly and can be overlooked. If there is too much blood on the slide, then the red blood cells might cover them up.
Can you place a few bacteria there and let us see how the WBC eats them?
love this
What actually happens or what do those cells when they seem to be boiling or being fried, bubbling and foaming?
probably the organelles and lysosomes moving in the cytoplasm
Very cool to see these white blood cells are still doing their work after leaving the body. How long do they typically remain alive after leaving the body?
Do they get energy from what they scavenge or did it burn energy to break it down 🤔
So what are those small fast moving "things" inside lecocytes?
@red dunkey You cant look at electrons from microscope.
I also see much smaller looking cells zooming around the red blood cells
I can only praise and say "Hallelujah"