To make a direct correlation between the drug conc and the the effect: more drug --> more %cytotox, but there are better tools to calculate the IC50, because making it a linear regression is not correct.
Dear one... When we calculate the MTT assay for the cancer cell such as MCF-7 and MDA-MB-231, we want our drug to be cytotoxic to these cells. That time we can use% cytotoxicity. Contrary to this when you experiment is about neuroprotection % cell viability will be more accurate. Further 100% cell viability is a theoritical concept generally we consider control group where there is no treatment was used have 100% cell viability. It would be wise to do the cell count to exactly know the cell density. Then again it's just a quick method. We have other more accurate methods like FITC, where we can exactly see how much cell are alive and how much cells are undergone apoptosis. Please check out my latest research to know more about MTT and FITC.... doi.org/10.1111/cbdd.14458
This was so helpful. Thankyou sm✨
about the linear regression, why dont use the %viability value? why %cytotoxic? and how it can be 100-%viability?
To make a direct correlation between the drug conc and the the effect: more drug --> more %cytotox, but there are better tools to calculate the IC50, because making it a linear regression is not correct.
Dear one... When we calculate the MTT assay for the cancer cell such as MCF-7 and MDA-MB-231, we want our drug to be cytotoxic to these cells. That time we can use% cytotoxicity. Contrary to this when you experiment is about neuroprotection % cell viability will be more accurate. Further 100% cell viability is a theoritical concept generally we consider control group where there is no treatment was used have 100% cell viability. It would be wise to do the cell count to exactly know the cell density. Then again it's just a quick method. We have other more accurate methods like FITC, where we can exactly see how much cell are alive and how much cells are undergone apoptosis. Please check out my latest research to know more about MTT and FITC.... doi.org/10.1111/cbdd.14458