Isolation of Chloroplast - Amrita University

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Chloroplasts are organelles 5-10 um in size in plants which perform the function of photosynthesis. These structures are mainly located in the palisade parenchyma of the mesophyll cells in the leaf. Chloroplasts belong to a family of specialized organelle called the plastid. The stem, leaves and unripened fruit in all the plants contain chloroplast. But leaves are major part of plants performing the process of photosynthesis.
The isolation of chloroplasts is typically carried out in dim light to minimize the photo-destruction of chlorophyll. Once the cells have been lysed, the separation of the various
components can start. A wide variety of techniques are available, including centrifugation, dialysis, salting out, chromatography, and electrophoresis.
Typically, a combination of approaches is used that removes some of the
unwanted material at each step.

[@Player1IsYourDad]

Are the chloroplast still "alive"? If yes, they still can do photosynthesis right?

[@clarerendle2605]

🇬🇧+🇺🇸=help

[@Alexander-bd2hk]

right