🔬 089 - How can you collect pond water for microscopy? | Microscopy

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  • เผยแพร่เมื่อ 23 ธ.ค. 2024

ความคิดเห็น • 65

  • @joshua_cr4948
    @joshua_cr4948 ปีที่แล้ว +5

    Right after getting a microscope I discovered your helpful channel and now I'm binging your backlog of videos. Thank you!!

  • @atharva-upclosingnature2423
    @atharva-upclosingnature2423 7 หลายเดือนก่อน +4

    I remember this day... 5 years ago... I was watching this video on 1st May 2019 early morning in my balcony and feeling the aroma of summer breeze... I was in middle school back then... happy and grateful as I look back how far I have come... microscopy is still my favorite hobby ... I have been watching Oliver's channel since it's very beginning... Jan-Feb 2018 I remember...😌 I have refreshed my memories now...❤

  • @jackywhat11
    @jackywhat11 5 ปีที่แล้ว +8

    this was so helpful, i have an environmental microbiology class this semester and one assignment is to collect pond water but i wasn't sure how to do it properly. Thanks for the tips!

  • @suzannepassero6621
    @suzannepassero6621 5 ปีที่แล้ว +3

    Excellent for high school biology students to view before collecting pond water samples for microorganism study under the microscope. Thank you!

  • @andredegraaf1643
    @andredegraaf1643 ปีที่แล้ว +1

    Thank you for the advice. I have been trying to collect a pond specimen for my daughter and we have come up with only some algae but no moving microbes. I will try again. Strange that when I was a kid, I had no problem collecting water with very interesting microscopic life. I even managed to get a hydra in the mix which I spotted with my naked eye.

  • @typicalarmadillo3238
    @typicalarmadillo3238 5 ปีที่แล้ว +7

    This is my favorite channel ever! I want to be a microbiologist Myself

  • @ChrisReyCap
    @ChrisReyCap 5 ปีที่แล้ว +4

    From reddit! I'm trying to get into microscopy so I'll stick around. Subbed!

  • @fernandoflores3161
    @fernandoflores3161 5 ปีที่แล้ว +2

    Thank you a lot for the tips! I will try them for my class at the university

  • @Mark-ks9jj
    @Mark-ks9jj 2 ปีที่แล้ว +1

    Great easy to follow information as usual

  • @kokamoe7082
    @kokamoe7082 5 ปีที่แล้ว +4

    Thanks man, you're the best

  • @autazm4782
    @autazm4782 5 ปีที่แล้ว +2

    Thanks, very useful

  • @AliHSyed
    @AliHSyed 5 ปีที่แล้ว +2

    Thanks for the hard work !

  • @MattyExplore
    @MattyExplore 5 ปีที่แล้ว +2

    Fantastic video, thank you very much

  • @ThisMicrophoneSoundsCheap
    @ThisMicrophoneSoundsCheap 5 ปีที่แล้ว +2

    I wonder what's the concentration of microbes in a typical "holy water font" (aspersorium) found in places of worship. I've seen examples with alarmingly high concentrations of algae. Those are touched by lots of people daily, which doesn't seem hygienic, especially in Summer, when the ambient temperature tops over 30 degrees Celsius. I'd be very interested in an experiment involving various techniques of making enrichment cultures from samples of holy water. Have you tried adding a pinch of sugar?

    • @Microbehunter
      @Microbehunter  5 ปีที่แล้ว +4

      While it is possible to make enrichment cultures of all sorts of water samples, the culture that you produce this way is not really characteristic of the original microorganisms that live there. Some will grow, others won't and it ultimately you will have a culture that has nothing to do with the original sample. WHat microbiologists do in this case is to take a water sample and plate it out on agar petridishes. This avoids the problems above as each individual bacterium will grow into its own colony. So there is no possibility of a few fast growing bacteria to take over the whole culture (and suppress the others). I remember that studies observing this water has been done and not surprisingly a lot of bacteria have been found. As many aspesoria are in churches which have a lower temperature, this might reduce bacterial growth but still....

    • @ThisMicrophoneSoundsCheap
      @ThisMicrophoneSoundsCheap 5 ปีที่แล้ว +2

      @@Microbehunter Thanks for a great answer. I hope to see a video where you experiment with different techniques using identical water samples (ideally from 2-3 sources). I imagine that a video where you'd do such an experiment on holy water samples has a very high potential of going viral, but you probably shouldn't provide precise information about their origin. I hope this isn't too controversial.

  • @OhMeOhMyCanItBe
    @OhMeOhMyCanItBe ปีที่แล้ว

    I have a question about the first way to make an enrichment culture: what kind of artificial fertilizer would you recommend? Thank you.

    • @Microbehunter
      @Microbehunter  ปีที่แล้ว

      For ciliates: add a crushed cereal grain. or algae a few drops of liquid flower fertilizer. th-cam.com/video/xq_qw9SkfuQ/w-d-xo.html

  • @Pervostolnik
    @Pervostolnik หลายเดือนก่อน

    Thanks!

  • @eqlzr2
    @eqlzr2 5 ปีที่แล้ว +1

    Hi Oliver. Yesterday I visited the Carrizo Plains National Monument on the San Andreas Fault in southern California. Soda Lake is locatedthere, and 99% of the lake is dry now because it's summertime. However, at one end there is a very small pond of water remaining, so I took a sample for microscopy. I thought maybe I would see some new/unusual microorganisms. Well, I was quite surprised. While there were not many microorganisms, I discovered myriad microscopic crystals, like tiny diamonds, suspended in the water. When the lake is dry, it is almost completely white on the surface due to the salts that have concentrated there over eons of time. It turns out the lake has been mined on and off for centuries for these salts, including thenardite (sodium sulfate) and halite (salt). Do you have any experience or comments on finding and seeing microscopic crystals in water samples? I wonder, for example, if all big crystals start as microscopic versions of their larger selves? Many thx.

  • @vijaygangurde6092
    @vijaygangurde6092 5 ปีที่แล้ว +1

    Your channel is somewhat like a boon for us. Keep it up.
    I request you to please make a video on plasmolysis( its in one of your magazine)

  • @ernestoabarca274
    @ernestoabarca274 4 ปีที่แล้ว

    What about creating and maintainig a microorganism zoo glass/jar?
    On my life, I had several times a glass for months with water/soil/died plants/sth rot to have it. But didn't get to know how to balance or to keep it really alive.
    Some way to have few glasses of: milk (bacteria source) + plants (algae) + dirt ?... To be able to rebalance the microzoo mix glass so it doesn't dies.

  • @saumilsunilshah5663
    @saumilsunilshah5663 5 ปีที่แล้ว +1

    5:38
    What are those
    I have caght them

    • @Microbehunter
      @Microbehunter  5 ปีที่แล้ว +2

      Rotifers. en.wikipedia.org/wiki/Rotifer

    • @saumilsunilshah5663
      @saumilsunilshah5663 5 ปีที่แล้ว

      @@Microbehunter thx
      I dont have a pond near me
      Have you tried foldsvope

  • @michaelfishstein6356
    @michaelfishstein6356 5 ปีที่แล้ว +1

    Any suggestions for finding Nematodes in soil?

  • @jose.a.velazquez-mendez00659
    @jose.a.velazquez-mendez00659 2 ปีที่แล้ว

    Hello ! I want You to know that i enjoyed watching your video.
    I am getting ready to purchase a microscope, to try to get familiar observing aquarium water microbiology, as well as water from a tiny creek, which sometimes dries out in the dry seasons, in Orsnge County, Ca.
    So, your advice is applicable to my situation. Thanks, again.

  • @asianwithahoodie
    @asianwithahoodie 3 ปีที่แล้ว

    does scooping the sediment directly from the bottom work too?

    • @Microbehunter
      @Microbehunter  3 ปีที่แล้ว

      yes. Any solid material from the fountain/pond will contain a biofilm with microorganisms.

  • @paulzoch3279
    @paulzoch3279 5 ปีที่แล้ว

    Where can I find the Enrichment Culture/ artificial fertilizer? Thanks

    • @Microbehunter
      @Microbehunter  5 ปีที่แล้ว

      Artificial fertilizer can be found in any shop selling gardening material. But do not over-use the fertilizer, some algae do not like too much.

  • @ANGamersTechAndInfo
    @ANGamersTechAndInfo 2 ปีที่แล้ว

    What can I see with my foldscope..😢😢😢😢I can't find anything and I even don't know how magnification it has please help.....🙏🙏🙏🙏

  • @zagaberoo
    @zagaberoo 5 ปีที่แล้ว

    Very helpful; I'll definitely give these a try!

  • @tmgee6574
    @tmgee6574 2 ปีที่แล้ว

    I live in Canada in the Rocky Mountains. Does anyone know if I will be able to collect microbes in early April before the algae blooms? EDIT: Just watched to the end. I'm going to try to culture some pondwater. Still, any advice would be great!

  • @sandravermes4219
    @sandravermes4219 ปีที่แล้ว

    What power are you viewing these organisms at?

    • @Microbehunter
      @Microbehunter  ปีที่แล้ว

      Water organisms can be observed best at a magnification from 100-max 400x. The higher the magnification the more difficult it is to follow them.

  • @lanasadiella2543
    @lanasadiella2543 5 ปีที่แล้ว +10

    I love your channel even though its completely irrelevant to me. Thank you for the videos!

    • @foolishgamer
      @foolishgamer 5 ปีที่แล้ว +1

      why is it irrelevant?

  • @holdyourhorses9842
    @holdyourhorses9842 2 ปีที่แล้ว

    If someone were very thirsty and desperate for water and drank some stream water, would it be dangerous to consume?

    • @Microbehunter
      @Microbehunter  2 ปีที่แล้ว

      This depends entirely on how clean the water is.

  • @rahulbindhu
    @rahulbindhu 5 ปีที่แล้ว +3

    I study engineering but i love microbiology

    • @Eltodofull
      @Eltodofull 5 ปีที่แล้ว +2

      I study bioengineering.

  • @reigninblood123
    @reigninblood123 8 หลายเดือนก่อน

    Could you piss in the jar as an artificial fertilizer?

  • @Guinea.Pig-Gaming
    @Guinea.Pig-Gaming 5 ปีที่แล้ว

    Is a creek a good idea, or should it be a river?

    • @Microbehunter
      @Microbehunter  5 ปีที่แล้ว

      Both works as long as the rocks etc are covered with algae, cyanobacteria etc. You might have to find a place where the water flow is not too fast. When old leaves (etc) collect and decompose then there will also be microbial growth there.

    • @Guinea.Pig-Gaming
      @Guinea.Pig-Gaming 5 ปีที่แล้ว

      @@Microbehunter Thanks! I live pretty much in a desert, but if I can find a river around my area, I'll be sure to try and collect some water and algae.

  • @valor36az
    @valor36az 5 ปีที่แล้ว

    great advice, minor correction @ 3:19 organism is called cyclops

  • @ronnykhalil
    @ronnykhalil 10 หลายเดือนก่อน

    🙏

  • @saumilsunilshah5663
    @saumilsunilshah5663 5 ปีที่แล้ว

    Try with foldscope

  • @AquaponicsRevolution
    @AquaponicsRevolution 3 ปีที่แล้ว +3

    Hello microbehunter,
    I have been watching your microbehunting videos and I have to say I am impressed.
    I also have a channel about aquaponics called "Aquaponics Revolution" and would love to interview you on my channel to share your experience on microbehunting.
    Please let me know if such collaboration seems interesting from your side.
    Anyway keep up the good work :)
    Passionately,
    Jonathan

  • @markusjungwirth2826
    @markusjungwirth2826 5 ปีที่แล้ว

    Toller Kanal mit eindrucksvollen Bildern.
    Sind sie Deutscher? Der Landschaft zu urteilen ja.
    Liebe Grüße der Markus.J

  • @Sans-fl4pe
    @Sans-fl4pe 5 ปีที่แล้ว

    1st everything

  • @andreacamilaa
    @andreacamilaa 4 ปีที่แล้ว +3

    Love your channel! I’ve been watching for years and I finally got a microscope!