Another genome sequencer - Roche 454 junior

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  • เผยแพร่เมื่อ 22 ก.ย. 2024
  • Later, smaller version of the Roche 454 genome sequencer, from 2009.
    Teardown of older model : • Roche 454 GS FLX+ DNA...

ความคิดเห็น • 87

  • @ovalwingnut
    @ovalwingnut 6 ปีที่แล้ว +1

    Xlint! Thank you for the ride-along :) I use to work with electronic surplus in the mid-80's (a million years ago). LOVED it! It was like Christmas every day. To imagine the cost to produce these devices and then they are sold for pennies by weight?.. heartbreak & joy all mixed into one. Cheers!

  • @NoName-bt3oy
    @NoName-bt3oy 7 ปีที่แล้ว +9

    Mike's teardowns are up there with the best, always are.
    Cheers Mike, ya legend!

  • @boonedockjourneyman7979
    @boonedockjourneyman7979 6 ปีที่แล้ว +2

    I don't believe anyone has done work of this quality. Simply amazing stuff. Thank you.

  • @christopherleubner6633
    @christopherleubner6633 4 หลายเดือนก่อน

    Best teardown channel. I used to fix up and modify stuff like this. The software used for biotech stuff is not the best and hard to find though. To get it to work with other florescent probes is usually as simple as changing the optical band pass filters. Was surprised it still had the HDD inside😮

  • @JackZimmermann
    @JackZimmermann 7 ปีที่แล้ว +4

    The fiber optic lens blew my mind! I first thought that you used some kind of greenscreen effect haha!

  • @rimooreg
    @rimooreg 4 ปีที่แล้ว

    Must say...you sir, score the COOLEST stuff I’ve ever seen. The mass spec was amazing. Thank you Mike!,

  • @itemushmush
    @itemushmush 7 ปีที่แล้ว

    One of the best disassemblies on YT! Interesting take down and analysis you've done

  • @LucasGarrow
    @LucasGarrow 7 ปีที่แล้ว +40

    26:18
    I'm pretty sure I'd buy just to have one of these.

    • @MrBowbalitic
      @MrBowbalitic 7 ปีที่แล้ว +9

      No kidding, that was probably the coolest part to me. Everything else was as awesome as always, but this was something I've never seen before.

    • @DatBlueHusky
      @DatBlueHusky 7 ปีที่แล้ว +2

      *starts searching on ebay*

    • @LucasGarrow
      @LucasGarrow 7 ปีที่แล้ว +2

      DAT 30GB
      *SEARCHING INTENSIFIES*

    • @tzimmermann
      @tzimmermann 7 ปีที่แล้ว +1

      I was thinking about DIYing one, but don't know where to start right now. This object is so cool!

    • @vincei4252
      @vincei4252 7 ปีที่แล้ว +3

      Agreed, the bonded fiber bundle is something I'd love to play with myself!

  • @jabelsjabels
    @jabelsjabels 7 ปีที่แล้ว +3

    I'm really terrified of what future art installation Mike has in mind with all this bioscience equipment...

  • @TheStevenWhiting
    @TheStevenWhiting 7 ปีที่แล้ว +2

    As good and interesting as always. Your videos are always good.

  • @danielaustin7643
    @danielaustin7643 5 ปีที่แล้ว +1

    i really like your scientific equipment teardowns i would love to see a teardown of an HPLC sytems all the liquids stuff is really interesting. a gas chromatograph would be interesting too

  • @Flapjackbatter
    @Flapjackbatter 7 ปีที่แล้ว +1

    Roche makes all the best stuff ; )

  • @compu85
    @compu85 7 ปีที่แล้ว

    If you know the MAC of a device, you can create a static ARP entry to a known IP. Then you can get into the device and change the IP.

  • @andljoy
    @andljoy 7 ปีที่แล้ว +4

    Finally a device with a web config page that just uses simple HTML and not some stupid javascrit crap , i am looking at you netgear.

    • @staglomagnifico5711
      @staglomagnifico5711 7 ปีที่แล้ว

      The status page updated every few seconds, so there's probably some stupid JavaScript crap involved.

    • @davidgiga1993
      @davidgiga1993 7 ปีที่แล้ว +4

      Nope, can be done with pure html using the meta tag.

  • @AtomicShrimp
    @AtomicShrimp 7 ปีที่แล้ว

    I always enjoy watching your teardowns. I am curious as to what you do with the reusable parts from these machines - such as stepper motors - do you resell them?

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว +3

      I have a huge pile of bits - not got around to ebaying - will be doing the Dunstable Downs electronics bootsale this year to offload as much as I can

  • @mikeissweet
    @mikeissweet 7 ปีที่แล้ว +4

    Great teardown, as usual! Any possibility of getting mass spectrometer part 2 in the future?

  • @DextersTechLab
    @DextersTechLab 7 ปีที่แล้ว +7

    Great video as usual Mike, it seems such a shame the ccd/cameras in these seem to have so limited use.

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว +2

      Yes - crazy high cost, minimal value.

    • @FennecTECH
      @FennecTECH 6 ปีที่แล้ว

      They are fun as hell to screw with if you can get them to connect directly to a PC

  • @spikester
    @spikester 7 ปีที่แล้ว +11

    Time to stop what I'm doing for a half hour.

  • @rkan2
    @rkan2 7 ปีที่แล้ว +4

    Connector @6:56 looks like a Mini-PCI (not Mini-PCI-e)

  • @MrDubje
    @MrDubje 7 ปีที่แล้ว

    12:05 Marlow Industries seems to specialize in peltier cooling, not to be confused with Watson-Marlow, which specializes in peristaltic pumps...

  • @movax20h
    @movax20h 4 ปีที่แล้ว

    Nice video. Also the design is much better than the previous one. The HDMI connector is probably for something else. The SoC on the board doesn't support HDMI really. I am also surprised that they used a fully blown mechanical SATA HDD, instead of few GB compact flash or SD card to boot from. Weird. Maybe they did that because the SoC do have SATA interface, so it was easy to just use that, instead of using PATA based CF.

  • @trustthewater
    @trustthewater 7 ปีที่แล้ว +2

    I'm sure someone else has mentioned it, but Wireshark is your friend. Start a capture and add the filter:
    eth.addr == --:--:--:--:--:--
    Mac address with the : between the pairs.
    I use it all the time for find odd bits of network equipment with an unknown IP.

  • @PlasmaHH
    @PlasmaHH 7 ปีที่แล้ว

    For checking ip addresses of unknown devices I recommend using the zmap tool, which is really fast, I have had similar issues with having to identify what address a router was set to and within minutes I got its IP

    • @KSSilenceAU
      @KSSilenceAU 7 ปีที่แล้ว

      I ended up "Sharking" (using wireshark to sniff) my network once to discover the IP address of a web managed switch that i forgot the IP address of (no console), and that wouldnt reset via the standard procedure, most of my stuff is cisco but this device isnt and yeah, surprises me they couldnt even put a console port on (even Linksys did on their SMB web managed switches).
      Found it by looking at some LLDP packets it was sending that obviously belonged to the device in question (HP Procurve Web managed switch), and one of the data fields in that LLDP packet was the devices management IP.

  • @blank7921
    @blank7921 7 ปีที่แล้ว +4

    I hate the Nonstandard use of standard connectors sometimes! At least it didn't blow up your monitor!

    • @dangrie158
      @dangrie158 7 ปีที่แล้ว

      Blank maybe it is hdmi but only supports a certain mode which is not supported by the display

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว +2

      Could be 0 tried 2 monitors, at least one of which I'd expect to show a a 'invald format' type error instead of just doing nothing.

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว +13

      Nonstandard use is fine as long as it's done in a way that doesn't cause smoke

  • @riiwind
    @riiwind 7 ปีที่แล้ว

    Bloody interesting stuff.

    • @dan_
      @dan_ 7 ปีที่แล้ว +2

      Patriotism is just as backwards and illogical as religion, if not more so.

    • @riiwind
      @riiwind 7 ปีที่แล้ว +1

      dan_tm time and a place you div

  • @ratshitpartners5757
    @ratshitpartners5757 6 ปีที่แล้ว

    exciting.

  • @christopherleubner6633
    @christopherleubner6633 4 หลายเดือนก่อน

    I would have kept the fiber array csmera module whole. Thise CCDs are incredibly sensitive but lower resolution. One way to get a better aspect ratio is to use a scanning mirror with it Did that with a very similar one out of a colony counter.

  • @xhighalert
    @xhighalert 7 ปีที่แล้ว

    Is there a possibility that there was image analysis/processing done? The other ARM chip is understandable for that but the FPGA is straight up stumping. Would love to see it in operation with a logic analyzer handy.

  • @agoodm
    @agoodm 7 ปีที่แล้ว +5

    Itd be interesting to see if data recovery was possible from that HDD - ie recover anything that might of been deleted. Willing to try if you send the drive to me.

    • @eigenvector7035
      @eigenvector7035 7 ปีที่แล้ว +2

      +1, photorec

    • @agoodm
      @agoodm 7 ปีที่แล้ว +1

      Programatór i like photo rec but it really is quite crude compared to the pro tools

  • @frtard
    @frtard 6 ปีที่แล้ว

    HOW DO YOU FIND SUCH AMAZING PIECES OF TECH FOR SO _LITTLE_??

  • @richardr3098
    @richardr3098 7 ปีที่แล้ว

    Thanks for doing the tare down, really cool. One slight criticism, when you're showing pcbs, could you sit them on a desk. The motion blur of the pcbs moving in your hands really irritates my eyes.

  • @glenslick2774
    @glenslick2774 7 ปีที่แล้ว

    Other than buying one of these just to tear it down and take a look, it there any reason to buy one of these now? Looks like almost all of the parts are so special purpose that it would be difficult to reuse them for anything else even if it was essentially free. I suppose at least the power supplies might be general purpose, but there are much cheaper and easier ways to get those.

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว +3

      The camera might be of use for astronomy, or perhaps microfocus x-ray use, but this one has a wierd aspect ratio, so may only be able to use part of the sensor.

  • @malinsg1
    @malinsg1 7 ปีที่แล้ว +1

    damn it.stuck in subway.cant watch.y u do dis mike?

  • @RedFathom
    @RedFathom 7 ปีที่แล้ว

    The other processor is probably for future upgrades using miniPci cards.

  • @sbreheny
    @sbreheny 7 ปีที่แล้ว

    Any idea why the 13.2V supply was measuring only 12V?

  • @blank7921
    @blank7921 7 ปีที่แล้ว

    Should try holding S1 while booting to see if that service hdmi activates?

  • @RupertHandford
    @RupertHandford 7 ปีที่แล้ว

    Would love the pumps and valves for a project if they are available?

  • @ellenmadden8343
    @ellenmadden8343 7 ปีที่แล้ว

    Part of me wonders if it dumps the data to the HDD while doing the test, then transfers it to the control PC. I can't see any other reason they would use a 250GB hard drive.

  • @SilentMono
    @SilentMono 7 ปีที่แล้ว +2

    Holy shit you've edited the buggery out of this to cut the time down I guess? Like when you're taking pc box at the back off, just editing out a second here and there.
    I enjoy watching it as a one take usually, but this is pretty good too :-)

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว +7

      I mostly edit to remove pauses, errors and boring stuff like unscrewing etc.

    • @SilentMono
      @SilentMono 7 ปีที่แล้ว

      Yeah, they tend not to bother me either way, I thoroughly enjoy watching your teardowns as they go into things properly and are a decent length, you even pushed me into the hardware side of things, I've bought a couple of RPIs and arduinos to hack on, one of which is going to become an SDR rig as I'm interested in POCSAG around hospitals etc

    • @andljoy
      @andljoy 7 ปีที่แล้ว +1

      I like me an un-screwing montage.

  • @gorak9000
    @gorak9000 7 ปีที่แล้ว +3

    Broken bond wire 6th from the left at 23:04

    • @gorak9000
      @gorak9000 7 ปีที่แล้ว +1

      and they're all broken a few seconds later anyway!

    • @TerryMcKean
      @TerryMcKean 7 ปีที่แล้ว

      Yup... good trouble-shooting job, gorak9000... too bad the repair tech that worked on that genome sequencer 20 years ago or whatever missed that broken wire... the lab could have gotten some more years of sequencing genomes and other kinds of gnomes with that set if that broken wire had been seen and hooked back up again. :-)

  • @nRADRUS
    @nRADRUS 7 ปีที่แล้ว

    why it become obsolete ?

  • @nexaentertainment2764
    @nexaentertainment2764 7 ปีที่แล้ว

    I've said it on other videos, but I've always wondered how safe it is to take these apart. In terms of chemicals and biohazards. Is there any serious risk? I mean you have no idea what chemicals or biowaste could be in there. I assume the labs would clean them out, but I've seen them on sale with ominous warnings like "It is the buyers responsibility to safely clean the unit" and other such ambiguous warnings. I've thought about buying some cheap ones just for the hell of it, but the warnings put me off of buying it.

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว

      I think stuff like this usually cleans itself after each run.

    • @classicmacintosh
      @classicmacintosh 7 ปีที่แล้ว

      @NexaEntertainment That, and the chemicals used only pose a threat if you were to get them in to an open wound. As long as you don't go licking it you will be fine

    • @nexaentertainment2764
      @nexaentertainment2764 7 ปีที่แล้ว

      Embarrassingly I hadn't thought of that. Would make sense. Although I suppose there are other units which are part of multi unit boxes/set ups that might not. Or broken units.

    • @nexaentertainment2764
      @nexaentertainment2764 7 ปีที่แล้ว +1

      Good to know. Admittedly I have no clue what kind of chemicals genome/dna sequencers use.

    • @jovangrbic97
      @jovangrbic97 5 ปีที่แล้ว +3

      @@nexaentertainment2764 mostly just buffered phosphate solutions and a few enzymes, they are trying to replicate the state within a living cell so the chemicals are basically the same as what would be in your body so quite harmless!

  • @teemoinvietnam1356
    @teemoinvietnam1356 7 ปีที่แล้ว

    Try to restore files from the HDD?

  • @avypath
    @avypath 6 ปีที่แล้ว

    Just looked up those LEMO connectors. Jesus Christ, what are those things made of? Why are they so expensive?!

    • @ovalwingnut
      @ovalwingnut 6 ปีที่แล้ว

      AN, I've never even heard of them.. Of course that's not saying much ;) Will have to check them out so I can add that to my resume.

  • @nraynaud
    @nraynaud 7 ปีที่แล้ว +1

    you can try to launch an IP address discovery with "arp -a"

  • @AureliusR
    @AureliusR 7 ปีที่แล้ว +1

    7:05 that is a SO-DIMM connector

    • @classicmacintosh
      @classicmacintosh 7 ปีที่แล้ว +1

      It's a Mini-PCI connector.
      Quite what they would be doing with a PCI bus on such a device however escapes me.

    • @tesla500
      @tesla500 7 ปีที่แล้ว +4

      Probably the case that the CPU had that bus so they brought it out for future upgrades

    • @mikeselectricstuff
      @mikeselectricstuff  7 ปีที่แล้ว +4

      Yeah - if you're doing a board that complex you REALLY don't want to have to add something like that later

    • @AureliusR
      @AureliusR 6 ปีที่แล้ว

      Do mini-pci connectors have the clips on the side like that? I have only ever seen them with no retention, and mounting holes in the board to screw the board down to.

  • @steveswan5714
    @steveswan5714 7 ปีที่แล้ว +1

    £150 worth of basically junk lol

    • @JBaughb
      @JBaughb 7 ปีที่แล้ว +3

      He could sell the linear slides and recycle the aluminum...maybe sell the stepper motors. He'll probably make back a good chunk of what he spent.

  • @solidamber
    @solidamber 7 ปีที่แล้ว

    please use the English term several and not the US "bunch of"

    • @MattOGormanSmith
      @MattOGormanSmith 7 ปีที่แล้ว +7

      I've got a lovely several coconuts.