Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on Instagram as @animatedbiologywitharpan. Install the app to follow my photos and videos. instagram.com/invites/contact/?i=1p41h314q3fv8&
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Hope i got that right. So we can use the colony for the pcr as it is without purification, because the impurities from the rest of the bacteria exist only in minute amounts compared to our repeatedly duplicated construct?
Ya sometimes having a crude extract is good enough.....colony pcr is very robust and inexpensive method because you don't really have to purify all the dna from a colony
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Question regarding cases where colonies are small, such as when growing in low temp conditions. Can you dilute the colony in water or broth, then use some for PCR and some for overnight culturing?
I normally do this by picking the colony, wash the tip in 50uL of water, then boil it 3 min, and centrifuge it. Then I use the remaining water in the PCR reaction. Can you do this without the boiling/centrifuge step?
Individual transformants can either be lysed in water with a short heating step or added directly to the PCR reaction and lysed during the initial heating step ( when the temperature reach 95 degree celcius).
If you find my channel useful please support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Dynamic instability occurs when the microtubule assembles and disassembles at one end only, while treadmilling occurs when one end polymerizes while the other end disassembles. ... This is due to the fact that within a living cell, many microtubules are tightly anchored at one end of the filament. Moreover treadmilling phenomenon is rare in cells
Great illustration and precise 3 min video! Very helpful thank you from Seattle, Washington!
Glad to hear that my content helped you. Please share my channel link with your friends and help me to reach big audience. Lots of love from India.
You are like a god you really save my day when i dont understand my lectures. Thank you so much ! ❤️
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Thankyou soo much for this wonderful video
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on Instagram as @animatedbiologywitharpan. Install the app to follow my photos and videos. instagram.com/invites/contact/?i=1p41h314q3fv8&
Great explanation, thanks!
Please share my channel link with your friends and help me to reach big audience
Thankyou.. it'll be useful for my seminar
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
@@animatedbiologywitharpan sure 💙
Thanks for your content
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Thank you so much for your explanation.
I can’t see any colony in my plate after transformation. Can you please give me any suggestions? 🌹🌹
You can take a detailed consultation @$5 for possible trouble shooting
Nice video
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Can you pls make a video on agro agrobacterium freeze thaw method transformation
Hope i got that right. So we can use the colony for the pcr as it is without purification, because the impurities from the rest of the bacteria exist only in minute amounts compared to our repeatedly duplicated construct?
Ya sometimes having a crude extract is good enough.....colony pcr is very robust and inexpensive method because you don't really have to purify all the dna from a colony
@@animatedbiologywitharpan Alright, thanks a lot! ^^
so, the difference between colony pcr and normal pcr is you don't need to isolated the dna from our target right?
Yes you are right.
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Do You recomend doing this, in the case of cloning a fragment for a real time pcr? Thx
Yes it can be done .
Question regarding cases where colonies are small, such as when growing in low temp conditions. Can you dilute the colony in water or broth, then use some for PCR and some for overnight culturing?
Yes it's possible
I normally do this by picking the colony, wash the tip in 50uL of water, then boil it 3 min, and centrifuge it. Then I use the remaining water in the PCR reaction. Can you do this without the boiling/centrifuge step?
Individual transformants can either be lysed in water with a short heating step or added directly to the PCR reaction and lysed during the initial heating step ( when the temperature reach 95 degree celcius).
If you find my channel useful please support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
How did you make master mix?
You mix everything in a tube
@@animatedbiologywitharpan Thanks!!
Trademilling or steady state kya alag alag process hota hai? In dono process mai kya difference hota hai
Dynamic instability occurs when the microtubule assembles and disassembles at one end only, while treadmilling occurs when one end polymerizes while the other end disassembles. ... This is due to the fact that within a living cell, many microtubules are tightly anchored at one end of the filament. Moreover treadmilling phenomenon is rare in cells
@@animatedbiologywitharpan thanku so so much for this information, no one explained like this❤️❤️❤️❤️❤️❤️
@@cartikey4687 share with your friends and help me to reach out to big audience.
What primers are used in this experiment?
Primers will depend on your plasmid construct and depend on which region do u want to amplify
at 1:55 , do i have to use template dna or not ? please i need an answer :'(
The template DNA would come from bacterial colony hence no need of purification of DNA from these colonies.
@@animatedbiologywitharpan so i dont need to use dna while preparing mastermix after selecting bacteria colonies, right?
@@animatedbiologywitharpan i get confused :')