The contribution from electroosomosis is much greater than the contribution from electrophoresis (mainly because of the excess positive charge on the diffusive layer that drags the entire buffer solution (positive, negative and neutral ions) towards the cathode. In other words, this electroosmosis is originated mainly because of the nature of the negative charges on the inner wall of the capillary, which then acts accordingly to the applied voltage. For example, if no high voltage is applied across the capillary, only a double layer will form which is still rich with excess positive charge in the diffusive part to compensate the excess negative charge.
You can't have the electrophoretic mobility without also having the electroosmotic flow. As soon as you apply the electric field to the capillary, you get both.
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It was Very helpful, Thank you
Best video I watched about this topic
U must be scientist now mam?
Your video is really great!!! Well done
Cleared a lot of doubts! Thank you!
Nice video, thanks!
thank you so much
great video ty _()_
I will chating some important
Sir when separation is based on difference in electrophoretic mobility then what is the need of electroosmotic flow?
The contribution from electroosomosis is much greater than the contribution from electrophoresis (mainly because of the excess positive charge on the diffusive layer that drags the entire buffer solution (positive, negative and neutral ions) towards the cathode. In other words, this electroosmosis is originated mainly because of the nature of the negative charges on the inner wall of the capillary, which then acts accordingly to the applied voltage. For example, if no high voltage is applied across the capillary, only a double layer will form which is still rich with excess positive charge in the diffusive part to compensate the excess negative charge.
You can't have the electrophoretic mobility without also having the electroosmotic flow. As soon as you apply the electric field to the capillary, you get both.
wsup bro, you ever considered making vids not restricted to material seen in 3rd year labs? alright nice talk