In vitro determination of Km and Vmax of amylase by double reciprocal plot or LB plot

[@lakshminair3349]

People like you make life science and the basics so clear! Thank you!

[@yusrakhatib9272]

Thank you so much ma'am....It makes the practical much easier for me. Once again a thank for putting this much efforts.

[@nareshbarik5384]

Thank u ma'am 🙏

[@keertisharma6358]

As you told to take alpha amylase enzyme concentration 0.1%, but if we will perform these experiments with saliva sample, then how can we determine the concentration

[@angulim4856]

Please make a video on DPPH assay..what if the solution is more basic

[@microbiologypractical6401]

Thank you so much madam , easy to explain for me

[@pradeepupadhyay8184]

Great Vinita ji

[@Iqrasdiary27]

We don't have enough amount of purified enzyme...how much concentration we can use of purified enzyme

[@bethavamsi7874]

Very nice explaination. Thank you

[@sandhyachahal7636]

Excellent explanation

[@microbiologypractical6401]

Please ,make a vedio on study effect of enzyme concentration on amylase activity.

[@Neel.A.B]

How is slope 0.00096

[@pradeepupadhyay8184]

Great mam 👃👃👃👃👃👃👃

[@AmandeepKaur-xv7ds]

Thanku sooooo muchhhh

[@Iqrasdiary27]

I was check the effect of different ph of buffers on alpha amylase activity.. optimum ph was tris hcl buffer ph 9

[@Iqrasdiary27]

So will i use this buffer instead of phosphate buffer?

[@pradeepupadhyay8184]

Plz help me for nitrogenase activity measurement. And I will pay for that plz plz mam i m needy

[@JaiswalSunny3762-lg6iu]

Maam aaap ka no. Kya hai mai thore se doubt puchhna hai

[@bijoymondal6005]

Thanks mam