ESTIMATION OF HEMOGLOBIN - SAHLI'S ACID-HEMATIN METHOD | HAEMATOLOGY LAB | PHYSIOLOGY

Estimation of Hemoglobin
- Sahli's Acid-Hematin Method
- Haematology Practical
- Physiology and Pathology
- Sahli's hemoglobinometer Instruments
- Procedure
- Viva questions and Answers
For available soft copy (pdf) files visit digital store:
drhardikmistry.myinstamojo.com
Follow on Instagram:
/ voirmed365
PHYSIOLOGY PRACTICALS LIST WITH VIDEO HYPERLINK (free download)
drhardikmistry.myinstamojo.co...
#hematology #physiology #hemoglobin

[@drhardikmistry]

HEMOGLOBIN THEORY LECTURE
th-cam.com/video/E-T_nqz2Ggk/w-d-xo.html

[@rabbit5802]

Thank you.

[@firojabanumansuri9743]

Sir , the best thing about your video is the questions / answers which are usually asked in viva .💚.....thank u sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@panda-pw6eg]

Yes sir

[@prity4521]

Tomorrow is my practical exam thanks for this video I am searching this kind of video in which I understood everything
Thanks once again sir 😊

[@drhardikmistry]

Best luck! Thanks. Please keep sharing.

[@syednashra.__]

Ughhh.. Finally found the video that i wanted.. Here procedure given is so easy to understand and write down on practical notebook... Tysm

[@swagatadas2557]

Sir your teaching process is so amazing. I want to be like you. You are my inspiration of medical field. Much love for you ❤❤.

[@sanem_khan]

Thank you so much. It was so helpful Ans very easy!!! Tmrw is my practical and you literally saved my time!!

[@drhardikmistry]

Thanks for your positive words. Please keep sharing.

[@rummufirdous9705]

Thank you so much sir I have my sem practical exam in few days and due to covid did not have chance to perform every practical practically this vedio made my understand visually and made my concept clear .Require more such vedios sir

[@drhardikmistry]

Best luck 👍🏻
Please keep sharing.

[@ritumattu4428]

Thanku so much sir. Great job. Having gud knowledge.. Great 👍

[@rukhsana2018]

FEMALES- 12-15gm/dL,
MALES- 14-17 gm/dL,
INFANTS- 16-18gm/dL,
NEWBORN- 20-24gm/dL

[@nirbhaykumarsingh]

I am first year vetenary student
Sir, Your video is very helpful for my LAB.

[@drhardikmistry]

Thanks. Please keep sharing.

[@medicKarim]

Sir congratulations to you for making this type of work well explained. I'm a Bangladeshi MBBS student. Love your dedication. 🎉 🎁

[@drhardikmistry]

Thanks. Please keep sharing.

[@deepaksingh-ut7lh]

Vividly explained ❤

[@31nikitakhairnar83]

Thanku sir🙏 ...I am frist year D. Farmacy student ..sir the best explained about ur this video is Q& Ans ..which are useful asked in viva 😇

[@drhardikmistry]

Thanks for your positive words. Please keep sharing.

[@rujulbhavsar8745]

Thank you very much sir....once I had missed my clg hematology practical but after watching your video, I could understand the whole practical. Thank you very much for your efforts...

[@drhardikmistry]

Thanks. Please keep sharing.

[@rujulbhavsar8745]

@@drhardikmistry sure sir...🤗🤗

[@suvethaduraisamy3821]

@@drhardikmistry which type of hb can be estimated by sahli method sir?

[@yesharaj5611]

Thank you so much sir . You covered everything and questions answers too. Thank you so much again.

[@drhardikmistry]

Thanks. Please keep sharing.

[@bshashidhar354]

I absent the this experiment in college .now I understand this by seeing video . thanks sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@arindam9446]

Very good explanation
Thanks 😘

[@rajasekar4239]

We have very useful video sir and again thank you so much sir

[@geekymedico.8099]

Thank u for making practical vedios sir...
It's very helpful...🙏🙏🙂🙂🙂

[@drhardikmistry]

Thanks. Please keep sharing.

[@riarathore274]

Thankyou
Nice explanation

[@candy13x]

The video is very informative...
Most importantly it contains questions that maybe asked in viva
Thankyou so much sir ✨

[@drhardikmistry]

Thanks. Please keep sharing.

[@alonymachor8163]

wow!
u did a great job.
thank you for the work done

[@drhardikmistry]

HEMOGLOBIN THEORY LECTURE
th-cam.com/video/E-T_nqz2Ggk/w-d-xo.html

[@nishitachaudhary9025]

Well done hardik thanks for providing us good material for achieving grt knowledge regarding practicals which are not doing by colleges ... thank you ❤️

[@drhardikmistry]

Thanks. Please keep sharing.

[@rajasekar4239]

You do nice explanation

[@prathikshanandhini1559]

Thank you for this video!!! really helpful

[@drhardikmistry]

Thanks. Please keep sharing.

[@user-ze6gs5zo3u]

Thank you sir 👌

[@pritikushwaha5487]

Thank u very much sir

[@springsofsoul3560]

Excellent sir.
thank you so much!

[@drhardikmistry]

Thanks. Please keep sharing.

[@akankshapriya2070]

Best video💕

[@shivanishivani4705]

well explained 👏

[@rajasekar4239]

Thank you sir

[@architadutta7344]

Thank you so much for this!

[@drhardikmistry]

Thanks. Please keep sharing.

[@pragyabharti5447]

Very very nicely explained sir..best vdo ever..thanku sir 😀😀😀😀👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻🔥🔥🔥🔥🔥✨✨✨

[@drhardikmistry]

Thanks. Please keep sharing.

[@arashdeepkour3361]

Awsmmm thank u 💯

[@user-hs1du1wo3e]

Excellent demonstration thanx alot. Pro.Dr.SABAH AZIZ J. AL-OBOSI. IRAQ.BAGHDAD

[@drhardikmistry]

Thank you sir. It's a big complement for me. Please share with your groups and related circles.

[@prathameshgole2145]

Very descriptive video. Thank you sir

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@mukaramkhan6918]

Thank you sir ❤

[@rebeccahmar1688]

It's very special

[@mdrummanuzzaman]

Best
Covering all aspects

[@drhardikmistry]

Thanks. Please keep sharing.

[@snehab6927]

Finally understood the practical

[@drhardikmistry]

Thanks. Please keep sharing.

[@aneeqahmad9100]

Can we use any other acid other than HCL for the estimation of hb? Why?

[@nightrider-qf5uw]

Thanks such an nice explanation ❤️❤️❤️👍🙏

[@drhardikmistry]

Thanks. Please keep sharing.

[@jismiabraham558]

Excellent sir..🙏🙏🙏🙏Thanks alot

[@drhardikmistry]

Thanks.

[@bravehearts9663]

Its really helpful.. thanks alot

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@sumayatahira7819]

Best..our beloved teacher

[@drhardikmistry]

Thanks. Please keep sharing.

[@uniquewhatsappstatus7527]

thank you sir.
detailed explanation....

[@drhardikmistry]

Thanks. Please keep sharing.

[@Sakshi_9588]

Thanks sir 🤗

[@bhargavisanapala8031]

Please upload a video on jugular venous pressure examination

[@30_gayatrigadariya35]

Thank you ! 😊👍🏻🙏🏻

[@drhardikmistry]

Thanks for being here. Share with your groups.

[@AB27editz]

Thanku Sir .Really helpful .❤️

[@drhardikmistry]

Thanks. Please keep sharing.

[@Abijith..]

Thankyou sir
Very well defined

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@mazharmhd1416]

Thank u sir. Very nice presentation

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@indianfashion2010]

Thanku sir it's too much helpfully for me

[@drhardikmistry]

Thanks. Please keep sharing.

[@aminadilawar2969]

Sir this is the best vedio. Everything isdescrib

[@drhardikmistry]

Thanks. Please keep sharing.

[@ganiyatmusa3225]

Thanks so much sir your lecture is easy to get

[@drhardikmistry]

Thanks. Please keep sharing.

[@Anjali-xe5uc]

​@@drhardikmistrysir when I bow off the blood in tube it didn't go down
I tried several times ...
Is it because of clotting?

[@vineethavini7990]

Helpfully video I like it 👍🏻

[@drhardikmistry]

Thanks. Please keep sharing.

[@user-ze6gs5zo3u]

It really helpful 🙏

[@drhardikmistry]

Thanks. Please keep sharing.

[@shinykj8536]

Thank you sir for the detailed explanation

[@drhardikmistry]

Thanks. Please keep sharing.

[@merinapanyang3150]

Best video ever

[@drhardikmistry]

Thanks. Please keep sharing.

[@athena777_]

You are amazing ✨✨✨.

[@drhardikmistry]

Thanks. Please keep sharing.

[@aryakanudawala4847]

Thank You Sir!!

[@drhardikmistry]

Thanks. Please keep sharing.

[@vijaypratapchoudhary763]

Very well explained 👏 👌

[@drhardikmistry]

Thanks. Please keep sharing.

[@prangyapritilipsapatra2593]

Sir plz uploading all methods.. Very nicely prepared this🤩

[@drhardikmistry]

For full hematology playlist Please visit: th-cam.com/play/PLcVgXSU9gf3vXvZnT6w9Iw3p7kKCL5Ymj.html
Thanks. Please keep sharing.

[@HannahsHaven84]

Sir excellent class ❤

[@drhardikmistry]

Thanks. Please keep sharing.

[@dishapatel7342]

Great explanation 🙂

[@drhardikmistry]

Thanks. Please keep sharing.

[@nayirarao5503]

Tqq very much sir this is veeeeery useful video

[@drhardikmistry]

Thanks. Please keep sharing.

[@supriyabhagat3161]

Sir while checking the color of the solution in comparator box should we keep the stirrer inside the solution or outside?

[@drhardikmistry]

Stirer should be above the solution but must be present inside the hb tube. Thanks. Please keep sharing.

[@mariem1522]

thank you!!

[@drhardikmistry]

Thanks. Please keep sharing.

[@hetvishah6641]

So much thank you💕

[@drhardikmistry]

Thanks. Please keep sharing.

[@bshashidhar354]

Super sir

[@akhilam626]

Very clearly explained

[@drhardikmistry]

Thanks. Please keep sharing.

[@karthikeyavanimireddy3693]

Thank you so much sir 😌💖 💖

[@drhardikmistry]

Thanks. Please keep sharing.

[@Vd_prabhat]

Thanku sirji💞

[@drhardikmistry]

Thanks. Please keep sharing.

[@Cherishable00]

Excellent

[@drhardikmistry]

Thanks for being here. Share videos with your groups.

[@aestheticqueen461]

Thank you so much

[@drhardikmistry]

Thanks. Please keep sharing.

[@mscreation3486]

Thank you sir.........

[@drhardikmistry]

Thanks. Please keep sharing.

[@manishajoyce4039]

Thank you sir👍

[@drhardikmistry]

Thanks. Please keep sharing.

[@shrutipathak2096]

Thanks alot sir😊🙏

[@drhardikmistry]

Thanks. Please keep sharing.

[@its_my_way4578]

Thank you sir😍😍

[@drhardikmistry]

Thanks. Please keep sharing.

[@AhmedKhaled-ou3dq]

Very good, doctor

[@drhardikmistry]

Thanks. Please keep sharing.

[@nibedita7445]

So helpful sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@hazel8537]

After putting hcl in the tube, should we mix the sucking blood sir

[@ramyaadevi6036]

Well explained

[@drhardikmistry]

Thanks. Please keep sharing.

[@bookyaravi6289]

Good explanation

[@drhardikmistry]

Thanks for being here. Please share these videos with your groups.

[@aalokshah6207]

Sir it’s colorimeteric comparision or calorimetric?

[@jadumonigogoi403]

What if you take N/10hcl upto 10 mark instead of 20 ?

[@pritamjitoinam8639]

Is the result obtain by sahli's method accurate? Or does the result obtain has some variation?

[@drhardikmistry]

No, it shows aproximate values. Thanks. Please keep sharing.

[@arpanbora8423]

Sir...this normal values are in accordance with which book??

[@aayshasaifi1115]

Osmm vdo sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@tharanginipeetlatharangini7821]

Tq so much sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@rukhsana2018]

Some more VIVA QNS OF Hb RELATED TO BOTH THEORY and PRACTICAL POINT OF VIEW--
I STUDIED THIS SO JUST THOUGHT OF SHARING WITH YOU GUYS.
1. fxns of HB? TRANSPORT OF OXYGEN AND CO2 , BUFFERING ACTION, ALSO STORES IRON
2. Hb is tetramer or dimer or monomer?
3. Hb contains how many heme groups?
4. 1 Hb can bind how many molecules of oxygen?
5. Adult Hb contains 2 alpha and 2 beta chains.
6. Components of HB-- 4 heme groups and 1 globin protein.
7. Why the oxygen dossoc curve is sigmoidal? -- explain about how binding of oxygen takes place with Hb by theory of cooperativity.
8. some hemoglobinopathies names you should know.-- methHbemia, sickle cell anemia , and thalassemia
9. what is heme? It is an iron containing pigment
10. where is Hb present? RBC OF THE BLOOD
11. hemoglobinuria? presence of hb in urine.
12. STR OF HB? each hb molecule consists of 4 heme groups and one globin protein molecule-- one heme molecule consists of one protoporphyrin ring and an iron molecule and the globin consists of four polypeptide chains (2pairs).
13. SYN OF HB? you should have a basic knowledge -- hb syn starts in PROERYTHROBLASTS. HEME is syn in cytosol and mitochondria and globin syn in ribosomes. (full pathway you must know for theory exams)
14. how protoporphyrin fromed? 4 pyrrole rings joined together to form one protoporphyrin rings.
15. TYPES OF HB IS MUST. TYPES OF HB IS BECZ OF? it is beacuse of differnet globin chains. (the heme group remains the same) .
16. ADULT HB (HbA)- 2alpha and 2 beta chains
17. FETAL HB (HbF) - 2 alpha and 2 gamma chains.
18. what is oxygen dissoc curve show? it shows % stauration of hb with partial pressure of oxygen.
19. what is p50? it is the partial pressure of oxygen at which 50% of oxygen binding sites are saturated.
20. SICKLE CELL ANEMIA-- point mutation, autosomal recessive, at 6th position of the beta globin chain of the Hb. glu changes to valine -- which results is sickle shaped rbcs-- which reduces the life span of the rbcs-- and thus causes destruction of rbcs and hence -- hemolytic anemia.
21- METHhemoglobinemia-- ferrous iron converted into ferric iron-- methhb cant bind oxygen-- results in chocolate cyanosis-- that is brownish discoloration of the skin and the muscous membranes.
22- Thalassemia - autosomal recessive, the defect is in the production of the globin chains-- either the alpha or the beta chains are not syn-- if alpha not syn then known as alpha thalassemia and if beta not syn then known as beta thalassemia.

[@Asquare369]

Awesome

[@drhardikmistry]

Thanks. Please keep sharing.

[@kirubadevi2582]

Sir why the stirrer should not be placed opposite side inverted

[@AbhishekNayak-qf5td]

Can you please tell the dimensions of thin stirrer rod

[@ishikahahaishika]

Thankyouuu

[@drhardikmistry]

Thanks. Please keep sharing.

[@surbhiv2024]

Vry nice sir 😊

[@drhardikmistry]

Thanks. Please keep sharing.

[@SkSahil_pharmacist]

thanks sir

[@drhardikmistry]

Share to your groups.

[@uyare2413]

Tnk u sir.... 😊😊

[@drhardikmistry]

Thanks. Please keep sharing.

[@mayurgarg7626]

Sir it's humble request pls 🙏 upload video on ESR

[@poojakurmi1305]

Thanq sir.

[@drhardikmistry]

Share to your groups. Thanks.

[@poojakurmi1305]

+DrHardik Mistry ok sir

[@smilygrl2545]

Kown kown merejaise practical exam ke pehle din ye vdo dekh raha he