ฝัง
- เผยแพร่เมื่อ 18 ก.ค. 2010
- ( www.abnova.com ) - Micropipettes are used to accurately measure and dispense small volumes of liquid. The capacity of a micropipette can range from less than 1μl to 1000μl (1ml). To ensure that obtained results using micropipette are reliable, it is necessary to calibrate micropipettes several times a year. This video shows a method for testing the accuracy of automatic micropipettes. More videos at Abnova www.abnova.com
- วิทยาศาสตร์และเทคโนโลยี
![คุณอาเรียโต๊ะข้างๆ พูดรัสเซียหวานใส่ซะหัวใจจะวาย ตอนที่ 2 [ซับไทย] 【Ani-One Thailand】](http://i.ytimg.com/vi/kbbfYakMY8k/mqdefault.jpg)
Isn't this video falsely titled? Shouldn't it be titled "testing the accuracy of micropipettes?"
It does not go into instructions on how to adjust the pipette should you get inaccurate results after testing for accuracy.
Exactly. Came here to recalibrate my lovely pipettes, was disappointed !
agreed
agreed
Where can this method of calculation literature be obtained,please answer immediately. Thank you.
cool! Thanks.
i think there is a mistake in calculation of accuracy No. 1 for the volumn 20 microlitre because if you calculate it it will equal 1 as following 20-19.8 =0.2 then 0.2 /20 =0.01 then 0.01 *100= 1.0 thats mean not like what you write 1.25 . when you review you will find the mistake exactly in the summation of the average because when you sum 19.8+19.6+19.7+19.9/4=19.75 not like what you write 19.8 , am i wright please answer me . thank you
Yes
saw that too. Useless.
The first column is wrong. For the 20ul the accyracuy is 1%., and that is provided that the average is correct. Whay are you doing over here?
GREAT
The water density equation is wrong, water is 1000kg/m^3
there some thing in your calculation sir
This is not a calibration video. At no point do you adjust the instrument for improved accuracy.
That can be done or not depending on the micropipette you are using. In the case of the olds transferpette, if you disaasmble it, you lose the calibration, so you have to fix a volume, meassure it as in the video, and then you can dissassemble the top to access a fixation that allows to change the dial without actually changing the aspirated volume, like using the clutch in a engine. Having the volume fixed and meassured (in water), you adjust the dial to the meassured volume and then validate that it's ok. That relies on the correct working of the screw that changes the volume by changing the depth that the plunger can travel. If you did what's described above, then change the volume, validate again and get a wrong measurement, the screw is what its bad and you'd have to change the pippete. Depends on what's wrong, somtimes you can buy some spare parts and fix what is wrong instead of changing the whole pipette. In the end depends on the brand and model, how mch error you can afford, and if yu have the money to buy another one or fix the current if possible.
I was unaware of the older models you are referring to. The pipettes we use all have to be sent back to the manufacturer if they no longer load their specified volume. This video is helpful in knowing *how* off the pipettes are, but not adjusting them. Unfortunately, that is up to the department's funding, and not my personal pique. *shrug*
yesss!!!
This video just tests the accuracy of the pipette. Have nothing to do with calibration. ffs ive wasted my precious 3 min