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The colors... Yikes

Can I use a SDS's picture from my smartphone camera to apply this method? Or I need to take a picture of SDS from specific machine?

Hi Rahul, I was wondering whether this method is working with bacteria cell suspension (I have gram positive bacteria), since if I am not wrong, we need to do lysis first and then DNA extraction? Do you have any lysis methode that can be couple with this DNA extraction methode? My worry whether there is some substance in the lysis methode that can hinder the extraction. I hope you can help me with my question. Thank you and best of luck.

Hi, Rahul, thank you for video. What do you think about in planta transformation of another Plant species?

I wish if the audio was better

thanks, you saved my major

Thank you so much; you have helped me a lot.

I'm running out of template DNA and I've discovered that it's degraded. This will be a perfect method for me to cut the target DNA band out of the gel, leaving behind the degraded product, and using the extracted DNA as PCR template after. Thanks!

thank u very much

Hi its really helpful but can you pls manage background noice

Hi! Thanks so much for sharing this and your general mission. I am hoping to try PCA extraction method for leeches, would this method here be applicable? Also, is there a lysis buffer recipe that you would recommend? Thank you!

Very helpful. thanks

Very helpful.

if i use Rf can I use the same equation and method?

Hi, As you mentioned , using the ratio phenol chloroform: sample is 1:10 is as good purity as 1:1. Do you have any articles showing that? Or it is just your own experience. I feel like 1:10 is too small and don’t know how purity of it compare to 1:1 for sequencing. What if I use ratio 1:2 or 1:5? I tried to use less chloroform but would like to be as good purity as possible. Thank you for a great video

Hi ... Its a flowless wonderful video.... But my practical was not gone like this ...as i seen in the video activation buffer was spread in the leaf ...but when i was performed it was stuck ...it was not spread .... why it happened...could you please reply

Does this works for PCR CDS product? I want to make restriction of a CDS into a plasmid.

Hello, your protocol was helpful for me, and I want to extract genome of mycoviruses from Botrytis cinerea fungus with PCI (phenol :chloroform: isoamyl alcohol ) method . can you help me? please give me your text of protocol.

Hi Rahul, im so thankful bout' your protocols. I found out your channel 27 minutes ago by the DNA purification by agarose gel, and then I came here to watch the purification. I'm going to do your protocols at school tomorrow, it'll be interesting to see how good can we perform. Your protocols are (at least teorethically) something that in LATAM we know as "3 B, Barato, Bueno, y Bonito", in english, Cheap, Good, and Pretty.

Can i do this step before DNA clean up? I want to send my sample for sequencing. And direct gel extract by using kit gave me like 10 ng/ul for PCR product.

Thank you so much thus the exact video I needed

May I know, what tool was used in the video??

Hello Sir, thank you so much for this informative video. I have a question though. Using MS-hyg selection, we are able to identify our transformants but how do we filter our homozygous tranformants from the heterozygous ones. Is the genetic ratio the only method available?

Hello, i did an extraction but i let the pellet dry at 37 °C for like 1 hour, so when i resuspended in TE buffer i couldnt dissolve it totally, i had clumps, can you help me?

for years I have seen ppl use "Analyse --> gel --> plot lane tool" method for quantification. I think I like this one better!

Hi Thanks for such a informational video. I was wondering do you maintain a specific OD (or you check OD of overnight grown culture) when you dissolve pellet into infiltration buffer?

Can you record a video how to insert this gRNA fragment within the plasmid between two location of a restriction enzymes? thanks

If I want to compare transgenic plants' growth with wild type, should I grow WT seeds at the same with non-antibiotic media or non-transformants can be retained as WT?

Plaese. I need the word file.. Can you send me?

How to design construct for gene knock in?

I can not print the protocol though :(

Amazing work, it was never easier to understand. Thank you so much

very nice video. Thank you very much!

Can we purify cDNA by this method after cDNA synthesis?

I was wondering what kind of filter tips you used? Can the filter material be the problem?

Thank you so much even tho this is 4 years old, It helped me with my biochemistry project. ❤🦭

The highsest peak i have after this method & nanodropping the results is at the 230 mark, is that still considered DNA? Or is it because of the presence of EtBr, Thank You

What is the name of the tool you have used for the cloning?

Hi! My class linked to your video for one of our labs in Lab Techniques in Plant Studies, and I really enjoyed it and learned quite a bit. I work in accessibility, and had a mild concern about the music in the background. I was wondering if you wouldn't mind posting a transcript somewhere, or I could provide you one? Someone who has low hearing might have trouble understanding the video.

At least tag them ! Which one is well watered?

Thanks for the amazing video, we have some old Freeze & Squeeze columns from Biorad. I wanted to know if I can use these columns for PCR clean up? Do you think the DNA will bind to the filter?

Is this protocol efficient enough for sequencing?

Hi Which software is best and why image j or gel analyzer for anlyzing gel i want quick response i m very worried can anyone help me plz in this difficult time.

Very good video 👍🏻

Pls can u tell me the name of the software u are using 🙏🙏🙏

Hello Dr. Patharkar, I have another question regarding LPA. I wonder if it interfere with following electroporation of ligated plasmid into electrocompetent E coli. Since the polycation is supposed to form stable complex with DNA. Also, seems that nobody has ever mentioned the average MW of LPA used for precipitation. I'm considering purchase the powder to make stock. Thank you so much.

I forgot to add Silwet before dipping. Is there any chance I will get transformants or should I start again?

Hi, Rahul. Long time no see. Thanks, great video.

Hey I have been doing this cloning but I am not getting any positive colonies. Can you please help me out

Nice video... I tried hygromycin selection and it worked perfectly 👍