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I need a secure generator box for boondocking. This should be a simple solution, we'll see after the first field test.

Inoculation of 1.5% nutrient agar @ 2% gelling strength with a multispore solution using a disposable cell spreader. 2 plates germinated while the others remained blank. When using a SAB or laminar flow, aseptic technique is extremely important, this is an example of that reality. Still air and laminar flow are advancements in the application of aseptic techniques that add layers of assurance, ...

The following is a combination of features adapted from Baba Yaga's manure water Tek, Mateja's coir/BRF recipe, PF Tek, V.L's EF-Tek, and in-vitro bottle Tek. Manure tea will be used to hydrate the coir/verm/brf recipe to avoid the requirement for grain spawn and to take advantage of all ingredient's ability to be steam-sterilized at atmospheric pressure in the same way we would process PF-Tek ...

The lid holes are actually 1/4"* Testing the load penetration capabilities of the 23-quart Presto pressure canner by inserting type 4 steam indicator strips into grain (un-cooked/un-hydrated) jars to ascertain both penetration and cycle function. One cubic foot of water will become 1646 cubic feet of steam when evaporated at zero psi gauge pressure and a temperature of 212°F. One cubic foot of ...

Don't make me spawn my foot in your ass.

Still air is the name of the game in a still still-air-box (SAB). A still internal atmosphere allows for microscopic contaminant particles to rest on the floor of the SAB, turbulence generated by movements within the "box" will disrupt the settled particles, potentially resulting in contamination. Never modify a SAB with gloves, blowers, or fans, filtered or otherwise. Do not fill your SAB with...

2" zinc machine screws, washers, and nuts. pretty straightforward. When loading quart or liter jars for mushroom cultivation, you will need to adjust the leg length to accommodate 10 jars in the 23q Presto. Leg length should be 1 1/4".

Plate transfers can be used to make plates intended to be used as inoculum, or for separtion from contamination (cleaning). One mater plate can produce many others for culture expansion.

Always use a proven, pure plate culture to inoculate liquid culture. Spores carry potential contaminant organisms which should be avoided at all costs. Simply place a myceliated wedge of agar into the vessel, place on a shelf at room temperature to colonize.

Always make sure to pull the mushroom fruitbody apart, don't cut it or you'll risk transferring surface contamination to the interior mushroom flesh. This process is the same for all cultivated species. With a sterilized scalpel, remove a small chunk of internal tissue and place it in the center of a petri dish, then wrap the plate and place it on a shelf at room temperature to colonize.

Autoclaves require weekly validation in a laboratory setting to ensure proper function and quality control. As home cultivators or business owners, we don't require this level of scrutiny, however, it may be important to verify your sterilizer initially when operating an atmospheric sterilizer for commercial production. When validating larger units, simply place a biological indicator ampoule i...

Agar is quite simple when dealing with non-selective media. Standard 2% gelling and nutrient strength are all that are required to produce healthy fungal colonies. Amending with peptones, yeasts, etc, is not necessary but if that's your thing, to each their own; for most of us this will be all you'll ever need: for a sleeve of 25 @ 2% concentration: 600ml distilled h20 12g agar 12g Dry malt ext...

Boiling produces the most reliable results when hydrating large cereal grains for spawn production. Boil until the starchy core has turned from white to translucent, then dry long enough for the surface to roll freely, and free of moisture. this process is the same for common large cereal grains used for spawn production: Wheat, oats, and rye berries. load jars with approximately 3 cups, then s...

Agar to grain transfer in a still air box (SAB)

How to sterilize mushroom spawn bags in a 23q Presto

How to inoculate and seal a 5lb sawdust block/spawn bag for home mushroom cultivation.

That time I verified the Instant Pot for use as an "autoclave".

Liquid culture (LC) montage

How to build a humidification system for a mushroom grow tent.

Cultivated outdoor wild Poplar mushrooms

How to grow mushrooms at home: Shoebox Tek

How to wrap Petri dishes with Parafilm

How to make filtered jar lids for mushroom grain spawn

How to use a spore swab for home mushroom cultivation.

How to pour and store blank Petri dishes for home mushroom cultivation.

How to fold and load spawn bags for home mushroom cultivation.

How to fold and load mushroom sawdust production blocks.

Growing mushrooms from spore print | How to use a cell spreader to inoculate agar.

A quick flash prep for millet grain spawn.