How it works: Solid Phase Reversible Immobilization (SPRI) technology

[@biancaleighhamman4234]

The process seems fairly simple, but how does the DNA bind to the beads? and how does the elution buffer separate the DNA from the beads again?

[@bappisarkar850]

The beads consist of a polystyrene core surrounded by a thin layer of magnetite, which makes it paramagnetic (i.e., the beads will only clump together under a magnetic field). On the surface, the bead is coated by carboxyl molecules, which provide the charge groups for DNA binding.The binding is reversible, in the elution step with the eluting buffer beads are separated from DNA.

[@oortclouddomicile]

How is it reversible though? Is the chemistry known or is it proprietary?

[@fy4w9p]

@@oortclouddomicile The surface of the superparamagnetic beads is actually made amphoteric during the manufacturing process (positively charged at low pH, negatively charged at high pH) The amphoteric surface allows them to reversibly bind DNA.

[@fy4w9p]

The surface of the beads is actually made amphoteric during the manufacturing process (positively charged at low pH, negatively charged at high pH). The amphoteric surface allows them to reversibly bind DNA.