FacebookLive: Finding Exon and Intron sequences

[@annlinjacob2182]

Thank you for doing this video. really helpful ! Please do such videos often

[@sguardian870]

Thank you. I have always outsourced primer/probe design because I didnt know how to find exons. This will help me so much. Thank you.

[@yusfiaurwatulwutsqa2615]

Thank you, this video is very useful for my thesis

[@OhEmGeeItsHolliee]

Thank you for the video! I imagine I'm too late with this question - but I was wondering if there's a way to get the transcription/pre-mRNA sequence directly from ensemble? TIA

[@sguardian870]

A t 2:29 if i want to design primers that span exons junctions how do i do this. Im confused. There are exons in yellow and then ESPN exons in red. And then just yellow in between. Would i need to pull together just the red letters or all letter in yellow.

[@EnsemblHelpdesk]

The yellow without the red text is exons of other genes. To design primers only for your gene of interest, you need to just look at the red sequence.

[@patelnikunjkumarprakashbha2107]

Thanks
It is very helpful ☺

[@malarmathi6670]

Thank you for this awesome video.

[@Ariinfrance]

wow! it really helped me. Thank you!

[@gdp2102]

It was useful video. Good work! please keep it up. Thank you

[@scienceforall4881]

Please, why in mRNA seq we're seeing nucleotides like DNA, have not U base?

[@kelebekaan2826]

Thanks for sharing. So, how can I get transcript structure of gene at Ensembl? Thanks again!

[@camilalara1545]

YOU ARE AN ANGEL

[@Garcia-elf]

wasn't there a way to view all the exons of the gene, not of a particular transcript?

[@renuvidyarthi7998]

I am unable to download the exon seq from this tool. No database is available for plants specially Oryza sativa,, help me in finding

[@laurasudupemedinilla759]

Thanks! It helped me a lot

[@anhnhan4868]

Thanks from Vietnam

[@sameerulsalam9745]

Nice explanation

[@zulhussnain7183]

What is the software or cite used

[@saniarauf6426]

How we download the entire gene sequence from 5' (ATG) to 3' (UAG).means start to stop codon?

[@EnsemblHelpdesk]

The Export data button on the genes pages provide you with options to download genomic, cDNA, CDS and protein sequences.

[@messatfamoussa9655]

Thank you for this awesome video... Please I need a help for find a sequence of a SNP plymorphism of TNF gene

[@EnsemblHelpdesk]

Please email details of your query to [e-mail] explaining exactly what it is you're struggling with.

[@shivbarik8738]

HOW CAN WE FIND A MUTATION IN A PARTICULAR EXON

[@EnsemblHelpdesk]

Ben shows you the configure menu for the exons view at around 7m40s. You can display variants using that option.