Could you help me Plzz... I'm student of organic chemistry gonna analyse my sample(hplc analysis) my sample is plant extract soluble in different organic solvent sach as dcm, ethyle acetate, hexane etc... Should I give my sample as it is in dissolved in different solvent or I have to ready it in some other way before analysis for hplc??
Must all of the components of the sample be polar for reverse phase HPLC? Especially, what do you do if your sample has polar and nonpolar components (i.e. that are not water soluble?)
Non-polar samples can also be analysed depending on the characteristics of the sample. I suggest u search reported methods of the compound and make the necessary adjustment.
Dear Dr. Aminu, this is a very clear instruction, thank you! Looking forward to see more instructional videos from you 🙏🏻
Hello Noafiu, thank you very much! I'm working as analist in a lab and this is so helpful.
Could you help me Plzz... I'm student of organic chemistry gonna analyse my sample(hplc analysis) my sample is plant extract soluble in different organic solvent sach as dcm, ethyle acetate, hexane etc... Should I give my sample as it is in dissolved in different solvent or I have to ready it in some other way before analysis for hplc??
It's quite informative. Thank you
Pls do not stop your work WELL DONE
Interesting guide to HPLC
# Very amazing Dr. Aminu!
Thank you Dr Aminu
very informative, thank you, Dr. Aminu.
Hello, what was the first Liquid you poured into the tube
Sir aachi video h
Great excellent
Thanks
Thank you for your help
Very usefull video, especially for those who start in HPLC (like me). Thanks!
Thanks
Very informative. Please what is the lyopolization time for aqueous extract. And how many minutes it takes for a successful injection. Thanks
Thanks Sir
Which type of septa used for hplc waters system
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Must all of the components of the sample be polar for reverse phase HPLC? Especially, what do you do if your sample has polar and nonpolar components (i.e. that are not water soluble?)
Non-polar samples can also be analysed depending on the characteristics of the sample. I suggest u search reported methods of the compound and make the necessary adjustment.
@@nafiuaminu2185 Thank you for the advice!
If sample is discoloured what we have to do, if chili powder analysis
No issue you can tested on different instrument
REALLY GOOD VIDEO,, I NEED HOW TO TEST MY SAMPLES USING LC
For top 10 HPLC question follow
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