Hi Sina, Yes, you can incubate a primary conjugated antibody overnight at 4°C while it’s protected from light. You can learn more with our written protocol: www.biolegend.com/protocols/immunohistochemistry-protocol-for-frozen-sections/4288/. If you have additional questions, please contact tech@biolegend.com.
how many time you took for each step when u whashed in PBS? when you used the solution to apply the coverglass is it ok that that solution touch the tissue in the slide?
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Greetings from Bulgaria
Amazing video
Are there any method can alter fluorescence stainning method? Cause my lab doesn't have fluorescence microscope
do you have any good protocol for FFPE human sections AND/OR human cryosections? thx
If the primary is conjugated, is it still okay yo incubate the slides with conjugated primary over night?
Hi Sina,
Yes, you can incubate a primary conjugated antibody overnight at 4°C while it’s protected from light. You can learn more with our written protocol: www.biolegend.com/protocols/immunohistochemistry-protocol-for-frozen-sections/4288/. If you have additional questions, please contact tech@biolegend.com.
how many time you took for each step when u whashed in PBS?
when you used the solution to apply the coverglass is it ok that that solution touch the tissue in the slide?
5 minutes each wash
Thank you so much!